2001
Systematic Determination of the Packaging Limit of Lentiviral Vectors
Kumar M, Keller B, Makalou N, Sutton R. Systematic Determination of the Packaging Limit of Lentiviral Vectors. Human Gene Therapy 2001, 12: 1893-1905. PMID: 11589831, DOI: 10.1089/104303401753153947.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsBlotting, SouthernCapsidCell LineDNAEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesGenetic VectorsHeLa CellsHIV-1HumansLentivirusMembrane GlycoproteinsModels, GeneticPlasmidsReverse Transcriptase Polymerase Chain ReactionRNA, ViralTransduction, GeneticTransfectionViral Envelope ProteinsConceptsPackaging limitLentiviral vectorsHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1Oncoretroviral vectorsTherapeutic deliveryVesicular stomatitis virus G proteinMeasurable titersLentiviral particlesLow titersViral titersType 1Great potentialForeign DNATitersProviral RNAVirus G proteinViral encapsidationHIVG proteinsLarge vectorsChromosomal DNA fragmentLimited utilityCells
1999
Transduction of Human Progenitor Hematopoietic Stem Cells by Human Immunodeficiency Virus Type 1-Based Vectors Is Cell Cycle Dependent
Sutton R, Reitsma M, Uchida N, Brown P. Transduction of Human Progenitor Hematopoietic Stem Cells by Human Immunodeficiency Virus Type 1-Based Vectors Is Cell Cycle Dependent. Journal Of Virology 1999, 73: 3649-3660. PMID: 10196257, PMCID: PMC104140, DOI: 10.1128/jvi.73.5.3649-3660.1999.Peer-Reviewed Original ResearchConceptsProgenitor hematopoietic stem cellsHematopoietic stem cellsCell cycleG0 cellsStem cellsTransduction ratesG2/M phaseReplication intermediatesCellular factorsHuman immunodeficiency virus type 1 vectorsTransduction efficiencyTransductionAddition of cytokinesM phaseHuman immunodeficiency virus type 1Immunodeficiency virus type 1Presence of cytokinesLentivirus vectorFurther characterizationReverse transcriptionDistinct subpopulationsG2/M fractionVirus type 1CellsTime of initiation
1998
Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells
Sutton R, Wu H, Rigg R, Böhnlein E, Brown P. Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells. Journal Of Virology 1998, 72: 5781-5788. PMID: 9621037, PMCID: PMC110379, DOI: 10.1128/jvi.72.7.5781-5788.1998.Peer-Reviewed Original ResearchConceptsHuman hematopoietic stem cellsHematopoietic stem cellsStem cellsMarker gene expressionLentivirus vector systemGene transfer agentsMarker gene productLong-term persistenceGene productsGene expressionHuman immunodeficiency virus type 1 vectorsIntegrated provirusStable expressionVector systemTransduction ratesCellsExpressionHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1MitosisTransductionDeletionOncoretrovirusesVpr