2001
Systematic Determination of the Packaging Limit of Lentiviral Vectors
Kumar M, Keller B, Makalou N, Sutton R. Systematic Determination of the Packaging Limit of Lentiviral Vectors. Human Gene Therapy 2001, 12: 1893-1905. PMID: 11589831, DOI: 10.1089/104303401753153947.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAnimalsBlotting, SouthernCapsidCell LineDNAEnzyme-Linked Immunosorbent AssayGene Transfer TechniquesGenetic VectorsHeLa CellsHIV-1HumansLentivirusMembrane GlycoproteinsModels, GeneticPlasmidsReverse Transcriptase Polymerase Chain ReactionRNA, ViralTransduction, GeneticTransfectionViral Envelope ProteinsConceptsPackaging limitLentiviral vectorsHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1Oncoretroviral vectorsTherapeutic deliveryVesicular stomatitis virus G proteinMeasurable titersLentiviral particlesLow titersViral titersType 1Great potentialForeign DNATitersProviral RNAVirus G proteinViral encapsidationHIVG proteinsLarge vectorsChromosomal DNA fragmentLimited utilityCells
2000
Transduction of Human PBMC-Derived Dendritic Cells and Macrophages by an HIV-1-Based Lentiviral Vector System
Schroers R, Sinha I, Segall H, Schmidt-Wolf I, Rooney C, Brenner M, Sutton R, Chen S. Transduction of Human PBMC-Derived Dendritic Cells and Macrophages by an HIV-1-Based Lentiviral Vector System. Molecular Therapy 2000, 1: 171-179. PMID: 10933928, DOI: 10.1006/mthe.2000.0027.Peer-Reviewed Original Research
1998
HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells
Uchida N, Sutton R, Friera A, He D, Reitsma M, Chang W, Veres G, Scollay R, Weissman I. HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 11939-11944. PMID: 9751769, PMCID: PMC21744, DOI: 10.1073/pnas.95.20.11939.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD34Base SequenceColony-Forming Units AssayDNA PrimersG1 PhaseGene ExpressionGene Transfer TechniquesGenes, ReporterGenetic TherapyGenetic VectorsGreen Fluorescent ProteinsHematopoietic Stem CellsHIV-1HumansIn Vitro TechniquesLeukemia Virus, MurineLuminescent ProteinsPhenotypeResting Phase, Cell CycleThy-1 AntigensTransduction, GeneticConceptsTransgene expressionHigh-efficiency gene transferSingle-step transductionGene delivery systemsGreen fluorescent proteinHematopoietic stem cellsHuman HSC subsetsVector supernatantsEfficient transductionTransduction efficiencyStem cellsHuman hematopoietic stem cellsMurine leukemia virusHIV vectorsDelivery systemGene transferGFP expressionHuman cell typesStem cell phenotypeReplication-defective HIVBone marrow stromal cellsLeukemia virusFluorescent proteinMarrow stromal cellsG0/G1Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells
Sutton R, Wu H, Rigg R, Böhnlein E, Brown P. Human Immunodeficiency Virus Type 1 Vectors Efficiently Transduce Human Hematopoietic Stem Cells. Journal Of Virology 1998, 72: 5781-5788. PMID: 9621037, PMCID: PMC110379, DOI: 10.1128/jvi.72.7.5781-5788.1998.Peer-Reviewed Original ResearchConceptsHuman hematopoietic stem cellsHematopoietic stem cellsStem cellsMarker gene expressionLentivirus vector systemGene transfer agentsMarker gene productLong-term persistenceGene productsGene expressionHuman immunodeficiency virus type 1 vectorsIntegrated provirusStable expressionVector systemTransduction ratesCellsExpressionHuman immunodeficiency virus type 1Immunodeficiency virus type 1Virus type 1MitosisTransductionDeletionOncoretrovirusesVpr