2006
Age‐related changes in the properties of α1‐adrenoceptors in the rat genitourinary tract
Yono M, Foster H, Yoshida M, Ueda S, Weiss R, Latifpour J. Age‐related changes in the properties of α1‐adrenoceptors in the rat genitourinary tract. The FASEB Journal 2006, 20: a254-a254. DOI: 10.1096/fasebj.20.4.a254-a.Peer-Reviewed Original ResearchLower urinary tract symptomsRat genitourinary tractBenign prostatic hyperplasiaΑ1-AR antagonistsAge-related changesMonth old ratsGenitourinary tractOld ratsBladder baseBladder domeDorsolateral prostateVentral prostateUrinary tract symptomsMaximum contractile responseLow testosterone levelsRadioligand receptor bindingSignificant age-dependent differencesReal-time RT-PCR techniqueAge-dependent differencesHigher body weightRT-PCR techniqueTract symptomsContractile responseOlder patientsSubtype mRNAs
2003
Developmental changes in the functional, biochemical and molecular properties of rat bladder endothelin receptors
Afiatpour P, Latifpour J, Takahashi W, Yono M, Foster HE, Ikeda K, Pouresmail M, Weiss RM. Developmental changes in the functional, biochemical and molecular properties of rat bladder endothelin receptors. Naunyn-Schmiedeberg's Archives Of Pharmacology 2003, 367: 462-472. PMID: 12700883, DOI: 10.1007/s00210-003-0715-6.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAutoradiographyBinding SitesEndothelin-1Gene ExpressionIodine RadioisotopesMuscle ContractionMuscle, SmoothRadioligand AssayRatsRats, Inbred F344Receptor, Endothelin AReceptor, Endothelin BReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTime FactorsUrinary BladderConceptsETA receptor subtypeSmooth muscle layerReceptor subtypesRat detrusorEndothelin receptorsET receptorsET-1Muscle layerSelective ETA receptor antagonistET receptor systemLarger contractile responsesTotal ET receptorsReceptor subtype mRNAsETA receptor antagonistETB receptor subtypesReal-time reverse transcription-polymerase chain reactionMuscle bath techniqueBladder smooth muscleReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionAge-dependent decreaseAge-dependent alterationsReal-time RT-PCRContractile responseSubtype mRNAs
2002
Quantification of endothelins, their receptors, and endothelin-converting enzyme mRNA in rat genitourinary tract using real-time RT-PCR
Yono M, Takahashi W, Pouresmail M, Johnson DR, Foster HE, Weiss RM, Latifpour J. Quantification of endothelins, their receptors, and endothelin-converting enzyme mRNA in rat genitourinary tract using real-time RT-PCR. Journal Of Pharmacological And Toxicological Methods 2002, 48: 87-95. PMID: 14565565, DOI: 10.1016/s1056-8719(03)00022-4.Peer-Reviewed Original ResearchConceptsRat genitourinary tractGenitourinary tractET-1MRNA expressionET-3Receptor subtype mRNA expressionReceptor subtype mRNAsETA receptor subtypeReal-time reverse transcription-polymerase chain reactionSubtype mRNA expressionReverse transcription-polymerase chain reactionReceptor-mediated mechanismTranscription-polymerase chain reactionReal-time RT-PCRSubtype mRNAsPolymerase chain reactionReceptor subtypesRat bladderReal-time PCRDorsolateral prostateReceptor mRNAVentral prostateECE-1Computer-assisted programVas deferensThe effect of castration on endothelins, their receptors and endothelin converting enzyme in rat prostate
Takahashi W, Afiatpour P, Foster H, Ikeda K, Wada Y, Weiss RM, Latifpour J. The effect of castration on endothelins, their receptors and endothelin converting enzyme in rat prostate. Naunyn-Schmiedeberg's Archives Of Pharmacology 2002, 366: 166-176. PMID: 12122504, DOI: 10.1007/s00210-002-0575-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAspartic Acid EndopeptidasesAutoradiographyDiabetes Mellitus, ExperimentalDNA PrimersEndothelin-1Endothelin-3Endothelin-Converting EnzymesGene ExpressionMaleMetalloendopeptidasesMicroscopyOrchiectomyOrgan SizeProstateProtein BindingRadioligand AssayRatsRats, Sprague-DawleyReceptor, Endothelin AReceptor, Endothelin BReceptors, EndothelinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerConceptsReceptor subtype mRNAsET receptor subtypesSerum testosterone levelsET receptorsRat prostateEffects of castrationReceptor subtypesSubtype mRNAsDorsolateral regionTestosterone levelsET-1Three-month-old SpraguePharmacological propertiesDiabetes-induced reductionET receptor systemET-3 mRNAETB receptor subtypesPost-operative dayReverse transcription-polymerase chain reactionVentral regionECE-1 mRNATranscription-polymerase chain reactionLight microscopy autoradiographyEnzyme-1 mRNAMultiplex reverse transcription-polymerase chain reaction