1999
Phosphorylating DNA with DNA
Li Y, Breaker R. Phosphorylating DNA with DNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 2746-2751. PMID: 10077582, PMCID: PMC15840, DOI: 10.1073/pnas.96.6.2746.Peer-Reviewed Original ResearchConceptsSubstrate recognition patternRandom sequence poolsKinase-like activityStandard NTPsATP hydrolysisSpecific target DNAVitro SelectionIndividual DNAProtein enzymesDNADNTP substratesMultiple turnoversDeoxyribozymesEnzymeTarget DNABiological systemsDeoxyribose moietyGTPNTPsRNAATPDNTPsCatalytic potentialSelectionRibose
1998
An amino acid as a cofactor for a catalytic polynucleotide
Roth A, Breaker R. An amino acid as a cofactor for a catalytic polynucleotide. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 6027-6031. PMID: 9600911, PMCID: PMC27579, DOI: 10.1073/pnas.95.11.6027.Peer-Reviewed Original ResearchConceptsAmino acidsRate enhancementDramatic rate enhancementMetal ion cofactorsAbsence of enzymeGeneral base catalystSuperior polymerChemical catalysisBiological catalystsRNA cleavage reactionCatalytic potentialOrganic cofactorImidazole groupsNatural ribozymesRNA worldBase catalystChemical groupsCatalytic mechanismCatalytic DNACleavage reactionIon cofactorProtein enzymesStructural foldingSubstrate cleavageConstituent amino acids