1999
Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group
Li Y, Breaker R. Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group. Journal Of The American Chemical Society 1999, 121: 5364-5372. DOI: 10.1021/ja990592p.Peer-Reviewed Original ResearchSpecific base catalysisBase catalysisHydroxyl groupsRNA phosphodiesterRate enhancementMaximum rate enhancementGeneral base catalysisEnzyme mimicsAlkaline pH conditionsHydroxide ionTransfer reactionsReaction conditionsCatalysisBase-catalyzed cleavageDivalent magnesiumRNA polymersDNA/RNARNA linkageTransesterification reactionPH conditionsCleavage mechanismStrand scissionRNA transesterificationBiological systemsDominant pathway
1994
Production of RNA by a polymerase protein encapsulated within phospholipid vesicles
Chakrabarti A, Breaker R, Joyce G, Deamer D. Production of RNA by a polymerase protein encapsulated within phospholipid vesicles. Journal Of Molecular Evolution 1994, 39: 555-559. PMID: 7528810, DOI: 10.1007/bf00160400.Peer-Reviewed Original ResearchConceptsRNA polymeraseTemplate-independent RNA polymeraseProduction of RNACatalyzed polymerization reactionsBoundary membraneMacromolecular catalystsRNA productsPolymerization reactionPolymerase proteinDimyristoyl phosphatidylcholine vesiclesPolyacrylamide gel electrophoresisEthidium bromide fluorescenceEarly cellsRNA polymersPhase transition temperatureSubstrate fluxPhospholipid vesiclesVesiclesFree enzymeSuch reactionsGel electrophoresisPhosphatidylcholine vesiclesComponent lipidsPolymeraseCatalyst