1999
Engineering precision RNA molecular switches
Soukup G, Breaker R. Engineering precision RNA molecular switches. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 3584-3589. PMID: 10097080, PMCID: PMC22337, DOI: 10.1073/pnas.96.7.3584.Peer-Reviewed Original ResearchConceptsRNA molecular switchMolecular switchGenetic control elementsEnzyme engineering strategiesRNA switchesReceptor domainConformational changesControl elementsEngineering strategiesStructural bridgeModular natureMolecular sensorsStructural reorganizationCorresponding ligandsRNARibozymeSwitchLigandsCatalyticReceptorsTripartite constructsReorganizationDomain
1998
Cleaving DNA with DNA
Carmi N, Balkhi S, Breaker R. Cleaving DNA with DNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2233-2237. PMID: 9482868, PMCID: PMC19303, DOI: 10.1073/pnas.95.5.2233.Peer-Reviewed Original Research
1997
Rational design of allosteric ribozymes
Tang J, Breaker R. Rational design of allosteric ribozymes. Cell Chemical Biology 1997, 4: 453-459. PMID: 9224568, DOI: 10.1016/s1074-5521(97)90197-6.Peer-Reviewed Original ResearchConceptsAllosteric regulationAllosteric ribozymesEffector moleculesProtein enzymesActive siteCatalytic ratePresence of dATPSelf-cleaving ribozymesSmall effector moleculesPresence of ATPSmall molecule receptorRational design strategyCellular processesEnzyme active siteAptamer domainAllosteric controlAllosteric enzymeCatalytic RNARNA aptamersConformational changesLigand moleculesMetabolic pathwaysCatalytic activityAllosteric hammerheadCatalytic features
1994
Emergence of a replicating species from an in vitro RNA evolution reaction.
Breaker R, Joyce G. Emergence of a replicating species from an in vitro RNA evolution reaction. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 6093-6097. PMID: 7517040, PMCID: PMC44144, DOI: 10.1073/pnas.91.13.6093.Peer-Reviewed Original ResearchConceptsRNA polymeraseDNA-dependent RNA polymeraseRNA polymerase promoter sequencePromoter sequencesEvolution experimentsRNA moleculesAcid sequenceRNA transcriptsSpecific nucleic acid sequencesTarget chemical reactionNucleic acid sequencesRNA replicationPolymeraseRNADNAReverse transcriptaseSelective amplificationDNA hairpinsSequence replicationChemical reactionsUnique classReplicationSequenceTranscriptaseParticular chemical reaction