2023
Microtechnologies for single-cell and spatial multi-omics
Deng Y, Bai Z, Fan R. Microtechnologies for single-cell and spatial multi-omics. Nature Reviews Bioengineering 2023, 1: 769-784. DOI: 10.1038/s44222-023-00084-y.Peer-Reviewed Original ResearchSuch single-cell dataGene regulatory networksMulti-omics assaysSingle-cell dataMulti-omics studiesSingle cellsContext of tissuesGenome scaleRegulatory networksOmics informationGene expressionOmics assaysCellular profilingSubcellular levelIntact tissueCellsFunctional stateAssaysOmicsMicroarrayTissueProfilingRegulationExpressionMicrofluidic platform
2020
An Integrated Dielectrophoresis-Trapping and Nanowell Transfer Approach to Enable Double-Sub-Poisson Single-Cell RNA Sequencing
Bai Z, Deng Y, Kim D, Chen Z, Xiao Y, Fan R. An Integrated Dielectrophoresis-Trapping and Nanowell Transfer Approach to Enable Double-Sub-Poisson Single-Cell RNA Sequencing. ACS Nano 2020, 14: 7412-7424. PMID: 32437127, DOI: 10.1021/acsnano.0c02953.Peer-Reviewed Original ResearchConceptsBead loadingLoading rateLoadingTransfer efficiencyHigh-yield loadingCell trappingNanowell arraysVersatile designSubsequent encapsulationDevicesMicrowell arrayTransfer approachCurrent technologyFundamental limitationsBeadsActive manipulationSingle cellsWellsArrayDielectrophoresisMicrofluidicsStochastic pairing