An Integrated Dielectrophoresis-Trapping and Nanowell Transfer Approach to Enable Double-Sub-Poisson Single-Cell RNA Sequencing
Bai Z, Deng Y, Kim D, Chen Z, Xiao Y, Fan R. An Integrated Dielectrophoresis-Trapping and Nanowell Transfer Approach to Enable Double-Sub-Poisson Single-Cell RNA Sequencing. ACS Nano 2020, 14: 7412-7424. PMID: 32437127, DOI: 10.1021/acsnano.0c02953.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsHigh-Throughput Nucleotide SequencingMiceMicrofluidicsSequence Analysis, RNASingle-Cell AnalysisConceptsBead loadingLoading rateLoadingTransfer efficiencyHigh-yield loadingCell trappingNanowell arraysVersatile designSubsequent encapsulationDevicesMicrowell arrayTransfer approachCurrent technologyFundamental limitationsBeadsActive manipulationSingle cellsWellsArrayDielectrophoresisMicrofluidicsStochastic pairing