2014
CyTOF supports efficient detection of immune cell subsets from small samples
Yao Y, Liu R, Shin MS, Trentalange M, Allore H, Nassar A, Kang I, Pober JS, Montgomery RR. CyTOF supports efficient detection of immune cell subsets from small samples. Journal Of Immunological Methods 2014, 415: 1-5. PMID: 25450003, PMCID: PMC4269324, DOI: 10.1016/j.jim.2014.10.010.Peer-Reviewed Original ResearchConceptsImmune cell subsetsCell subsetsImmune cell statesPatient biopsiesTranslational investigationsFlow cytometryClinical researchCellular analysisMass cytometryMultiple cell populationsCell populationsCytometryCyTOFSingle-cell analysisMultiparameter single cell analysisFluorescence cytometryFluorescence-based flow cytometryCell statesHuman diseasesMarkersTremendous detailBiopsyPathogenesisInnate Immune Responses in the Neutrophils of Community Dwelling and Nursing Home Elders
Juthani-Mehta M, Guo X, Shaw AC, Towle V, Ning Y, Wang X, Allore HG, Fikrig E, Montgomery RR. Innate Immune Responses in the Neutrophils of Community Dwelling and Nursing Home Elders. Journal Of Aging Science 2014, 02 PMID: 25750929, PMCID: PMC4348001, DOI: 10.4172/2329-8847.1000115.Peer-Reviewed Original ResearchNursing home residentsΒ2-integrin expressionToll-like receptorsHome residentsInnate immune responseImmune responseIntegrin CD11bDisabled nursing home residentsIntegrin expressionΒ2 integrin CD11bLevels of chemokinesObservational pilot studyArea nursing homesNursing home eldersLocomotion of neutrophilsTLR4 responseChemokine inductionInfectious disease susceptibilityCommunity dwellingCommunity dwellersNursing homesEfficiency of neutrophilsNeutrophilsUnstimulated neutrophilsFlow cytometry
2010
Circulating monocytes from systemic sclerosis patients with interstitial lung disease show an enhanced profibrotic phenotype
Mathai SK, Gulati M, Peng X, Russell TR, Shaw AC, Rubinowitz AN, Murray LA, Siner JM, Antin-Ozerkis DE, Montgomery RR, Reilkoff RA, Bucala RJ, Herzog EL. Circulating monocytes from systemic sclerosis patients with interstitial lung disease show an enhanced profibrotic phenotype. Laboratory Investigation 2010, 90: 812-823. PMID: 20404807, PMCID: PMC3682419, DOI: 10.1038/labinvest.2010.73.Peer-Reviewed Original ResearchConceptsInterstitial lung diseaseSSc-ILD patientsSSc-ILDIL-10Normal controlsProfibrotic cellsSystemic sclerosisLung diseaseCollagen-producing cellsMCP-1Profibrotic phenotypeSSc-related interstitial lung diseaseFlow cytometryPeripheral blood profilesSSc-ILD cohortsIL-10 secretionSystemic sclerosis patientsExpression of CD163Blood of patientsHealthy aged controlsCultured CD14Profibrotic characteristicsProfibrotic mediatorsTNF levelsSclerosis patients
2007
Age-Associated Defect in Human TLR-1/2 Function
van Duin D, Mohanty S, Thomas V, Ginter S, Montgomery RR, Fikrig E, Allore HG, Medzhitov R, Shaw AC. Age-Associated Defect in Human TLR-1/2 Function. The Journal Of Immunology 2007, 178: 970-975. PMID: 17202359, DOI: 10.4049/jimmunol.178.2.970.Peer-Reviewed Original ResearchConceptsTLR1 surface expressionCytokine productionTLR functionTNF-alphaSurface expressionMultivariable mixed-effects modelsOlder adultsImpaired vaccine responsesTLR2 surface expressionInfection-related morbidityPeripheral blood monocytesYears of ageVaccine responsesIL-6Aged miceBlood monocytesYoung controlsFlow cytometryMixed effects modelsAge categoriesTLR1/2TLREffects modelN-palmitoylAdults