2021
Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes
Ravindra NG, Alfajaro MM, Gasque V, Huston NC, Wan H, Szigeti-Buck K, Yasumoto Y, Greaney AM, Habet V, Chow RD, Chen JS, Wei J, Filler RB, Wang B, Wang G, Niklason LE, Montgomery RR, Eisenbarth SC, Chen S, Williams A, Iwasaki A, Horvath TL, Foxman EF, Pierce RW, Pyle AM, van Dijk D, Wilen CB. Single-cell longitudinal analysis of SARS-CoV-2 infection in human airway epithelium identifies target cells, alterations in gene expression, and cell state changes. PLOS Biology 2021, 19: e3001143. PMID: 33730024, PMCID: PMC8007021, DOI: 10.1371/journal.pbio.3001143.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionSARS-CoV-2Human bronchial epithelial cellsInterferon-stimulated genesCell state changesAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSyndrome coronavirus 2 infectionCell tropismCoronavirus 2 infectionCoronavirus disease 2019Onset of infectionCell-intrinsic expressionCourse of infectionAir-liquid interface culturesHost-viral interactionsBronchial epithelial cellsSingle-cell RNA sequencingCell typesIL-1Disease 2019Human airwaysDevelopment of therapeuticsDrug AdministrationViral replication
2001
Murine Lyme Disease: No Evidence for Active Immune Down-Regulation in Resolving or Subclinical Infection
Montgomery R, Wang X, Malawista S. Murine Lyme Disease: No Evidence for Active Immune Down-Regulation in Resolving or Subclinical Infection. The Journal Of Infectious Diseases 2001, 183: 1631-1637. PMID: 11343212, DOI: 10.1086/320703.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferi GroupDisease Models, AnimalFemaleFluorescent Antibody TechniqueInterleukin-1Interleukin-12Lyme DiseaseMacrophage ActivationMacrophagesMiceMyocarditisPeritoneumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSpecific Pathogen-Free OrganismsTumor Necrosis Factor-alphaConceptsActive diseaseAnti-inflammatory cytokine IL-10Proinflammatory cytokines IL-1Levels of proinflammatoryAnti-inflammatory cytokinesCytokine IL-10Double-label immunofluorescent stainingCytokines IL-1Reverse transcription-polymerase chain reaction analysisSemiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysisTranscription-polymerase chain reaction analysisInfected heartsIL-10IL-12Inflammatory activityCytokine expressionTumor necrosisLocal macrophagesIL-1Peritoneal cavitySubclinical infectionChain reaction analysisLevels of mRNAMacrophage activationImmune system