2021
Single cell immunophenotyping of the skin lesion erythema migrans Identifies IgM memory B cells
Jiang R, Meng H, Raddassi K, Fleming I, Hoehn KB, Dardick KR, Belperron AA, Montgomery RR, Shalek AK, Hafler DA, Kleinstein SH, Bockenstedt LK. Single cell immunophenotyping of the skin lesion erythema migrans Identifies IgM memory B cells. JCI Insight 2021, 6: e148035. PMID: 34061047, PMCID: PMC8262471, DOI: 10.1172/jci.insight.148035.Peer-Reviewed Original ResearchConceptsMemory B cellsErythema migransB cellsEM lesionsIgM memory B cellsLyme diseaseB-cell receptor sequencingSkin infection siteCell receptor sequencingEarly Lyme diseaseLocal antigen presentationSkin immune responsesB cell populationsSingle-cell immunophenotypingMHC class II genesUninvolved skinImmune cellsSpirochetal infectionAntigen presentationCell immunophenotypingT cellsImmune responseIsotype usageAntibody productionInitial signs
2020
Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF
Stewart E, Wang X, Chupp GL, Montgomery RR. Profiling cellular heterogeneity in asthma with single cell multiparameter CyTOF. Journal Of Leukocyte Biology 2020, 108: 1555-1564. PMID: 32911570, PMCID: PMC8087109, DOI: 10.1002/jlb.5ma0720-770rr.Peer-Reviewed Original ResearchConceptsAirway immune cellsSource of inflammationChronic inflammatory diseaseInflammatory cell typesStudy of pathogenesisMultiple distinct subtypesHeterogeneity of diseaseBronchial epithelial cellsAirway inflammationCell typesAsthmatic patientsClinical responseEosinophilic infiltrateAnalysis of sputumAsthmatic inflammationFunctional statusClinical managementImmune cellsInflammatory diseasesHealthy controlsInflammatory responseAirway cellsSputumDistinct subtypesInflammationMolecular MRI of the Immuno-Metabolic Interplay in a Rabbit Liver Tumor Model: A Biomarker for Resistance Mechanisms in Tumor-targeted Therapy?
Savic LJ, Doemel LA, Schobert IT, Montgomery RR, Joshi N, Walsh JJ, Santana J, Pekurovsky V, Zhang X, Lin M, Adam L, Boustani A, Duncan J, Leng L, Bucala RJ, Goldberg SN, Hyder F, Coman D, Chapiro J. Molecular MRI of the Immuno-Metabolic Interplay in a Rabbit Liver Tumor Model: A Biomarker for Resistance Mechanisms in Tumor-targeted Therapy? Radiology 2020, 296: 575-583. PMID: 32633675, PMCID: PMC7434651, DOI: 10.1148/radiol.2020200373.Peer-Reviewed Original ResearchConceptsImmuno-oncologic therapiesConventional transarterial chemoembolizationTransarterial chemoembolizationIntratumoral immune cell infiltrationMR spectroscopyRabbit liver tumor modelPrussian blue iron stainingAntigen-presenting immune cellsIntra-arterial infusionImmune cell infiltrationNew Zealand white rabbitsLiver tumor modelImmune cell exclusionLiver cancer modelContrast material administrationT2-weighted MRIZealand white rabbitsT2-weighted imagingResistance mechanismsImmunosuppressive tumorHLA-DRCell infiltrationImmune cellsImmunohistochemistry stainingRing enhancementHow Inflammation Blunts Innate Immunity in Aging
Goldberg EL, Shaw AC, Montgomery RR. How Inflammation Blunts Innate Immunity in Aging. Interdisciplinary Topics In Gerontology And Geriatrics 2020, 43: 1-17. PMID: 32294641, PMCID: PMC8063508, DOI: 10.1159/000504480.Peer-Reviewed Original ResearchConceptsImmune responseInnate immunityPoor vaccine responsesInnate immune cellsFunctional immune responsesResolution of inflammationInnate immune responseBioactive lipid mediatorsSeverity of infectionImpaired tissue repairInnate immune systemInflammation influencesInflammatory changesLymph nodesVaccine responsesChronic inflammationImmune cellsImmune protectionImmune responsivenessAntigen presentationLipid mediatorsCytokine dynamicsTissue surveillanceImmune systemMolecular dysregulationSingle cell immune profiling of dengue virus patients reveals intact immune responses to Zika virus with enrichment of innate immune signatures
Zhao Y, Amodio M, Vander Wyk B, Gerritsen B, Kumar MM, van Dijk D, Moon K, Wang X, Malawista A, Richards MM, Cahill ME, Desai A, Sivadasan J, Venkataswamy MM, Ravi V, Fikrig E, Kumar P, Kleinstein SH, Krishnaswamy S, Montgomery RR. Single cell immune profiling of dengue virus patients reveals intact immune responses to Zika virus with enrichment of innate immune signatures. PLOS Neglected Tropical Diseases 2020, 14: e0008112. PMID: 32150565, PMCID: PMC7082063, DOI: 10.1371/journal.pntd.0008112.Peer-Reviewed Original ResearchConceptsZika virusCell subsetsDengue virusConcurrent dengue infectionInnate cell responsesInnate immune signaturesVirus-infected individualsDivergent clinical outcomesMosquito-borne human pathogenIntact immune responsePre-existing infectionInnate cell typesSingle-cell immune profilingPublic health importanceCell typesImmune signaturesVirus patientsWest Nile virusAcute patientsClinical outcomesImmune profilingDengue infectionImmune statusFunctional statusImmune cells
2019
Multi‐site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system
Bagwell CB, Hunsberger B, Hill B, Herbert D, Bray C, Selvanantham T, Li S, Villasboas JC, Pavelko K, Strausbauch M, Rahman A, Kelly G, Asgharzadeh S, Gomez‐Cabrero A, Behbehani G, Chang H, Lyberger J, Montgomery R, Zhao Y, Inokuma M, Goldberger O, Stelzer G. Multi‐site reproducibility of a human immunophenotyping assay in whole blood and peripheral blood mononuclear cells preparations using CyTOF technology coupled with Maxpar Pathsetter, an automated data analysis system. Cytometry Part B Clinical Cytometry 2019, 98: 146-160. PMID: 31758746, PMCID: PMC7543682, DOI: 10.1002/cyto.b.21858.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsWhole bloodPeripheral blood mononuclear cell preparationsDeep immune phenotypingBlood mononuclear cellsImmune cell populationsMononuclear cell preparationsCell populationsTranslational clinical researchWhole blood preparationsImmune phenotypingMononuclear cellsPBMC samplesClinical trialsImmune cellsClinical researchCyTOF technologyBlood preparationsInter-site reproducibilityBloodCell preparationsSingle donorMulti-site reproducibilityCellsProfiling assaysDevelopment of a 2-dimensional atlas of the human kidney with imaging mass cytometry
Singh N, Avigan ZM, Kliegel JA, Shuch BM, Montgomery RR, Moeckel GW, Cantley LG. Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry. JCI Insight 2019, 4: e129477. PMID: 31217358, PMCID: PMC6629112, DOI: 10.1172/jci.insight.129477.Peer-Reviewed Original ResearchConceptsCell typesIndividual cell typesCritical baseline dataRenal cell typesMass cytometryQuantitative atlasNormal human samplesHuman kidneyRelative abundanceDevelopment of therapiesHuman kidney diseaseKidney diseaseMetal-conjugated antibodiesQuantitative interrogationScarce samplesMachine-learning pipelineDiscovery purposesFuture quantitative analysisNovel abnormalityNormal human kidneySingle tissue sectionHuman samplesRenal biopsyImmune cellsCells
2017
AGE-RELATED CHANGES IN INNATE IMMUNE CELL FUNCTION
Montgomery R. AGE-RELATED CHANGES IN INNATE IMMUNE CELL FUNCTION. Innovation In Aging 2017, 1: 1254-1254. PMCID: PMC6183893, DOI: 10.1093/geroni/igx004.4559.Peer-Reviewed Original ResearchInnate immune cellsImmune cellsInnate immune cell functionLevels of TLR5Natural killer cellsToll-like receptorsImmune cell functionAge-associated decreasePotential therapeutic opportunitiesAge-related changesVaccine responsesKiller cellsVaccine strategiesOlder donorsImmune functionOlder subjectsTherapeutic opportunitiesInfectious diseasesProgressive declinePrimary humanCell functionMass cytometrySurface expressionReduced levelsReduced production
2016
Age-related alterations in immune responses to West Nile virus infection
Montgomery R. Age-related alterations in immune responses to West Nile virus infection. Clinical & Experimental Immunology 2016, 187: 26-34. PMID: 27612657, PMCID: PMC5167051, DOI: 10.1111/cei.12863.Peer-Reviewed Original ResearchConceptsWest Nile virusToll-like receptorsDendritic cellsSevere diseaseBlood-brain barrier permeabilityImportant public health concernWest Nile virus infectionHuman dendritic cellsNatural killer cellsAnti-viral responseMonocytes/macrophagesPathogen recognition receptorsAge-related alterationsSpecific immune parametersPublic health concernImportant causative agentAge-related impairmentIndividual host factorsAge-related changesKiller cellsViral encephalitisImmune cellsRisk factorsBarrier permeabilityHigh prevalenceRole of Immune Aging in Susceptibility to West Nile Virus
Yao Y, Montgomery RR. Role of Immune Aging in Susceptibility to West Nile Virus. Methods In Molecular Biology 2016, 1435: 235-247. PMID: 27188562, PMCID: PMC4941816, DOI: 10.1007/978-1-4939-3670-0_18.Peer-Reviewed Original ResearchConceptsWest Nile virusImmune dysregulationWNV infectionSevere neuroinvasive diseaseInnate immune cellsΓδ T cellsNile virusProminent risk factorAge-dependent dysregulationAge-related alterationsDendritic cellsNK cellsImmune agingNeuroinvasive diseaseImmune cellsRisk factorsT cellsImmune responseSpecific treatmentTherapeutic interventionsOlder peopleInfectionMass cytometryHost susceptibilityDysregulation
2009
Toll-like Receptor 7 Mitigates Lethal West Nile Encephalitis via Interleukin 23-Dependent Immune Cell Infiltration and Homing
Town T, Bai F, Wang T, Kaplan AT, Qian F, Montgomery RR, Anderson JF, Flavell RA, Fikrig E. Toll-like Receptor 7 Mitigates Lethal West Nile Encephalitis via Interleukin 23-Dependent Immune Cell Infiltration and Homing. Immunity 2009, 30: 242-253. PMID: 19200759, PMCID: PMC2707901, DOI: 10.1016/j.immuni.2008.11.012.Peer-Reviewed Original ResearchConceptsToll-like receptor 7West Nile virusReceptor 7WNV infectionImmune cell infiltrationLethal WNV infectionMyeloid differentiation factorIL-23 p19IL-23 responsesIL-12 p40West Nile encephalitisIL-12 p35Infected target cellsHost defense mechanismsRNA flavivirusInnate cytokinesWNV encephalitisInterleukin-12Cell infiltrationImmune cellsTarget organsVariable severityMiceTarget cellsTissue concentrations
2004
Myeloid Differentiation Antigen 88 Deficiency Impairs Pathogen Clearance but Does Not Alter Inflammation in Borrelia burgdorferi-Infected Mice
Liu N, Montgomery RR, Barthold SW, Bockenstedt LK. Myeloid Differentiation Antigen 88 Deficiency Impairs Pathogen Clearance but Does Not Alter Inflammation in Borrelia burgdorferi-Infected Mice. Infection And Immunity 2004, 72: 3195-3203. PMID: 15155621, PMCID: PMC415708, DOI: 10.1128/iai.72.6.3195-3203.2004.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAntibodies, BacterialAntigens, DifferentiationArthritisBorrelia burgdorferiDNA, BacterialInflammationLyme DiseaseMacrophages, PeritonealMiceMice, Inbred C57BLMice, KnockoutMyeloid Differentiation Factor 88MyocarditisOpsonin ProteinsPhagocytosisReceptors, ImmunologicUrineConceptsToll-like receptor 2Days of infectionPathogen burdenWT miceAcute inflammationB. burgdorferi-specific antibodyPathogen-specific adaptive immunityMyD88-dependent signaling pathwaysTumor necrosis factor alphaBurgdorferi-specific antibodiesImmunoglobulin G1 responsesTLR2-deficient miceInnate immune cellsBorrelia burgdorferiNecrosis factor alphaWild-type miceIgM titersImmune cellsInflammatory responseFactor alphaAdaptive immunitySpirochete Borrelia burgdorferiWT macrophagesReceptor 2Pathogen clearance