2023
Early cellular and molecular signatures correlate with severity of West Nile virus infection
Lee H, Zhao Y, Fleming I, Mehta S, Wang X, Vander Wyk B, Ronca S, Kang H, Chou C, Fatou B, Smolen K, Levy O, Clish C, Xavier R, Steen H, Hafler D, Love J, Shalek A, Guan L, Murray K, Kleinstein S, Montgomery R. Early cellular and molecular signatures correlate with severity of West Nile virus infection. IScience 2023, 26: 108387. PMID: 38047068, PMCID: PMC10692672, DOI: 10.1016/j.isci.2023.108387.Peer-Reviewed Original ResearchWest Nile virusEffective anti-viral responseInnate immune cell typesWest Nile virus infectionPro-inflammatory markersAcute time pointsImmune cell typesAnti-viral responseMolecular signaturesHost cellular activitiesAcute infectionAsymptomatic donorsPeripheral bloodSevere infectionsVirus infectionImmune responseSevere casesCell activityIll individualsSerum proteomicsInfectionInfection severityHigh expressionTime pointsNile virusProteome Analysis for Inflammation Related to Acute and Convalescent Infection
Sigdel T, Sur S, Boada P, McDermott S, Arlehamn C, Murray K, Bockenstedt L, Kerwin M, Reed E, Harris E, Stuart K, Peters B, Sesma A, Montgomery R, Sarwal M. Proteome Analysis for Inflammation Related to Acute and Convalescent Infection. Inflammation 2023, 47: 346-362. PMID: 37831367, PMCID: PMC10799112, DOI: 10.1007/s10753-023-01913-3.Peer-Reviewed Original ResearchC motif chemokine ligand 1C motif chemokine receptor 7Human Immunology Project ConsortiumWest Nile virusDengue virusLyme diseaseKidney transplant patientsChemokine ligand 1Chemokine receptor 7Common therapeutic interventionTumor necrosis factor receptorHost defense mechanismsNecrosis factor receptorCell surface markersConvalescent infectionTransplant patientsConvalescent phaseImmune signaturesAcute phaseConvalescent stageReceptor 7Common biological pathwaysHealthy donorsPolyomavirus infectionImmune response
2020
In-Depth Analysis of Genetic Variation Associated with Severe West Nile Viral Disease
Cahill ME, Loeb M, Dewan AT, Montgomery RR. In-Depth Analysis of Genetic Variation Associated with Severe West Nile Viral Disease. Vaccines 2020, 8: 744. PMID: 33302579, PMCID: PMC7768385, DOI: 10.3390/vaccines8040744.Peer-Reviewed Original ResearchAdditional novel variantsWest Nile virusNovel genetic variantsComprehensive genetic studiesGenetic Variation AssociatedGenetic architectureGene-gene interaction analysisNovel lociGene targetsLocus analysisBiological roleGenetic studiesGenetic variantsVirus datasetCell linesVariation AssociatedSevere West Nile neuroinvasive diseaseNovel variantsMosquito-borne virusViable targetViral diseasesNile virusInteraction analysisGenesLociSingle cell immune profiling of dengue virus patients reveals intact immune responses to Zika virus with enrichment of innate immune signatures
Zhao Y, Amodio M, Vander Wyk B, Gerritsen B, Kumar MM, van Dijk D, Moon K, Wang X, Malawista A, Richards MM, Cahill ME, Desai A, Sivadasan J, Venkataswamy MM, Ravi V, Fikrig E, Kumar P, Kleinstein SH, Krishnaswamy S, Montgomery RR. Single cell immune profiling of dengue virus patients reveals intact immune responses to Zika virus with enrichment of innate immune signatures. PLOS Neglected Tropical Diseases 2020, 14: e0008112. PMID: 32150565, PMCID: PMC7082063, DOI: 10.1371/journal.pntd.0008112.Peer-Reviewed Original ResearchConceptsZika virusCell subsetsDengue virusConcurrent dengue infectionInnate cell responsesInnate immune signaturesVirus-infected individualsDivergent clinical outcomesMosquito-borne human pathogenIntact immune responsePre-existing infectionInnate cell typesSingle-cell immune profilingPublic health importanceCell typesImmune signaturesVirus patientsWest Nile virusAcute patientsClinical outcomesImmune profilingDengue infectionImmune statusFunctional statusImmune cells
2018
Identification of genetic variants associated with dengue or West Nile virus disease: a systematic review and meta-analysis
Cahill ME, Conley S, DeWan AT, Montgomery RR. Identification of genetic variants associated with dengue or West Nile virus disease: a systematic review and meta-analysis. BMC Infectious Diseases 2018, 18: 282. PMID: 29929468, PMCID: PMC6014009, DOI: 10.1186/s12879-018-3186-6.Peer-Reviewed Original ResearchConceptsWest Nile virus diseaseSevere diseaseVirus diseaseWest Nile virus infectionGenetic factorsGenetic variantsSevere disease outcomesPotential therapeutic interventionsGenetic risk factorsAdditional genetic factorsWest Nile virusMinority of individualsSymptomatic infectionAsymptomatic infectionMechanisms of resistanceRisk factorsImmune mechanismsInitial symptomsDisease outcomeVirus infectionImmune responseDengue diseaseDisease pathogenesisTherapeutic interventionsSystematic review
2017
The natural killer cell response to West Nile virus in young and old individuals with or without a prior history of infection
Yao Y, Strauss-Albee DM, Zhou JQ, Malawista A, Garcia MN, Murray KO, Blish CA, Montgomery RR. The natural killer cell response to West Nile virus in young and old individuals with or without a prior history of infection. PLOS ONE 2017, 12: e0172625. PMID: 28235099, PMCID: PMC5325267, DOI: 10.1371/journal.pone.0172625.Peer-Reviewed Original ResearchMeSH KeywordsAdultAge FactorsAgedAged, 80 and overAntigens, CDAsymptomatic DiseasesFemaleGene Expression RegulationHumansImmunity, InnateImmunophenotypingInterferon-gammaKiller Cells, NaturalLymphocyte ActivationLymphocyte CountMiddle AgedNatural Cytotoxicity Triggering Receptor 1Natural Cytotoxicity Triggering Receptor 2Natural Cytotoxicity Triggering Receptor 3NK Cell Lectin-Like Receptor Subfamily CNK Cell Lectin-Like Receptor Subfamily KPrimary Cell CultureSeverity of Illness IndexWest Nile FeverWest Nile virusConceptsNK cell subsetsNK cellsWest Nile virusWNV infectionCell subsetsCell responsesSpecific NK cell subsetsNatural killer cell responsesInnate NK cellsSevere neuroinvasive diseaseNK cell responsesNK cell receptorsNile virusHuman WNV infectionsImmune pathogenesisNK repertoirePolyfunctional responsesMore IFNSymptomatic infectionChemokine secretionAsymptomatic infectionNeuroinvasive diseasePrior historyCytolytic activityInfection
2016
Age-related alterations in immune responses to West Nile virus infection
Montgomery R. Age-related alterations in immune responses to West Nile virus infection. Clinical & Experimental Immunology 2016, 187: 26-34. PMID: 27612657, PMCID: PMC5167051, DOI: 10.1111/cei.12863.Peer-Reviewed Original ResearchConceptsWest Nile virusToll-like receptorsDendritic cellsSevere diseaseBlood-brain barrier permeabilityImportant public health concernWest Nile virus infectionHuman dendritic cellsNatural killer cellsAnti-viral responseMonocytes/macrophagesPathogen recognition receptorsAge-related alterationsSpecific immune parametersPublic health concernImportant causative agentAge-related impairmentIndividual host factorsAge-related changesKiller cellsViral encephalitisImmune cellsRisk factorsBarrier permeabilityHigh prevalenceRole of Immune Aging in Susceptibility to West Nile Virus
Yao Y, Montgomery RR. Role of Immune Aging in Susceptibility to West Nile Virus. Methods In Molecular Biology 2016, 1435: 235-247. PMID: 27188562, PMCID: PMC4941816, DOI: 10.1007/978-1-4939-3670-0_18.Peer-Reviewed Original ResearchConceptsWest Nile virusImmune dysregulationWNV infectionSevere neuroinvasive diseaseInnate immune cellsΓδ T cellsNile virusProminent risk factorAge-dependent dysregulationAge-related alterationsDendritic cellsNK cellsImmune agingNeuroinvasive diseaseImmune cellsRisk factorsT cellsImmune responseSpecific treatmentTherapeutic interventionsOlder peopleInfectionMass cytometryHost susceptibilityDysregulation
2015
Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing
Tsioris K, Gupta NT, Ogunniyi AO, Zimnisky RM, Qian F, Yao Y, Wang X, Stern JN, Chari R, Briggs AW, Clouser CR, Vigneault F, Church GM, Garcia MN, Murray KO, Montgomery RR, Kleinstein SH, Love JC. Neutralizing antibodies against West Nile virus identified directly from human B cells by single-cell analysis and next generation sequencing. Integrative Biology 2015, 7: 1587-1597. PMID: 26481611, PMCID: PMC4754972, DOI: 10.1039/c5ib00169b.Peer-Reviewed Original ResearchConceptsHumoral responseNext-generation sequencingB cellsWest Nile virus infectionSevere neurological illnessMemory B cellsAntibody-secreting cellsCohort of subjectsWNV-specific antibodiesHuman B cellsMosquito-borne diseaseWest Nile virusAnamnestic responseAntibody responseAvailable treatmentsClinical severityAntibody isotypesNeurological illnessVaccine studiesVirus infectionGeneration sequencingInfectious diseasesPrevious exposureTherapeutic antibodiesAntibodiesAssociation between high expression macrophage migration inhibitory factor (MIF) alleles and West Nile virus encephalitis
Das R, Loughran K, Murchison C, Qian F, Leng L, Song Y, Montgomery RR, Loeb M, Bucala R. Association between high expression macrophage migration inhibitory factor (MIF) alleles and West Nile virus encephalitis. Cytokine 2015, 78: 51-54. PMID: 26638028, PMCID: PMC4696904, DOI: 10.1016/j.cyto.2015.11.021.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorWNV encephalitisWest Nile virusHigh-expression MIF allelesWest Nile virus encephalitisNeuroinvasive WNV diseaseCase-control studyMigration inhibitory factorNorth American patientsMIF locusWNV neuropathogenesisVirus encephalitisViral neuroinvasionInnate cytokinesMIF allelesAmerican patientsTherapeutic targetAnimal modelsWNV diseaseEncephalitisInhibitory factorFunctional polymorphismsPatientsNile virusImportant determinantHuman NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility
Strauss-Albee DM, Fukuyama J, Liang EC, Yao Y, Jarrell JA, Drake AL, Kinuthia J, Montgomery RR, John-Stewart G, Holmes S, Blish CA. Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility. Science Translational Medicine 2015, 7: 297ra115. PMID: 26203083, PMCID: PMC4547537, DOI: 10.1126/scitranslmed.aac5722.Peer-Reviewed Original ResearchConceptsAntiviral responseInnate natural killer (NK) cellsNK cell repertoire diversityHIV-1 acquisitionNatural killer cellsOutcome of infectionNK cellsWest Nile virusAntitumor responseKiller cellsCytokine productionInhibitory receptorsImmune historyImmune experienceHIV-1Repertoire diversityViral susceptibilityNile virusAfrican womenExposure riskFunctional consequencesTerminal differentiationRiskSingle-cell levelCellsRisk factors for West Nile virus infection and disease in populations and individuals
Montgomery RR, Murray KO. Risk factors for West Nile virus infection and disease in populations and individuals. Expert Review Of Anti-infective Therapy 2015, 13: 317-325. PMID: 25637260, PMCID: PMC4939899, DOI: 10.1586/14787210.2015.1007043.Peer-Reviewed Original ResearchConceptsWest Nile virusWest Nile virus infectionComplex immune interactionsRisk factorsAdvanced ageVirus infectionImmune responseSevere diseaseImmune interactionsClinical casesMosquito-borneCDC reportNile virusNaïve bird populationsInfectionDiseasePositive-strand RNA virusesRNA virusesVirusNew York CityHypertensionImmunosuppressionPopulationYork City
2014
Immune Markers Associated with Host Susceptibility to Infection with West Nile Virus
Qian F, Thakar J, Yuan X, Nolan M, Murray KO, Lee WT, Wong SJ, Meng H, Fikrig E, Kleinstein SH, Montgomery RR. Immune Markers Associated with Host Susceptibility to Infection with West Nile Virus. Viral Immunology 2014, 27: 39-47. PMID: 24605787, PMCID: PMC3949440, DOI: 10.1089/vim.2013.0074.Peer-Reviewed Original ResearchConceptsWest Nile virusSevere infectionsImmune markersIL-4IL-4 levelsSerum cytokine levelsSerum IL-4Nile virusSignificant risk factorsImmune system statusPeripheral blood cellsSevere neurological diseaseCytokine levelsAntibody levelsImmune statusRisk factorsHealthy subjectsStratified cohortWNV infectionNeurological diseasesInfectionAltered expression levelsBlood cellsAltered gene expression patternsHost susceptibility
2013
Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis
Qian F, Chung L, Zheng W, Bruno V, Alexander RP, Wang Z, Wang X, Kurscheid S, Zhao H, Fikrig E, Gerstein M, Snyder M, Montgomery RR. Identification of Genes Critical for Resistance to Infection by West Nile Virus Using RNA-Seq Analysis. Viruses 2013, 5: 1664-1681. PMID: 23881275, PMCID: PMC3738954, DOI: 10.3390/v5071664.Peer-Reviewed Original ResearchConceptsCommon gene pathwaysNovel cellular responsesDifferential gene expressionRNA-seq analysisWest Nile virusGene expression analysisPrimary human macrophagesGene isoformsHigh-throughput methodRNA-seqGene pathwaysExpression analysisGenes CriticalKnock-downGene expressionCellular responsesGene changesResistant individualsBiological settingsHuman macrophagesGenesCritical roleAvailable treatmentsHealthy donorsViral infection
2012
West Nile Virus: Biology, Transmission, and Human Infection
Colpitts TM, Conway MJ, Montgomery RR, Fikrig E. West Nile Virus: Biology, Transmission, and Human Infection. Clinical Microbiology Reviews 2012, 25: 635-648. PMID: 23034323, PMCID: PMC3485754, DOI: 10.1128/cmr.00045-12.Peer-Reviewed Original ResearchQuantitative imaging of lineage-specific Toll-like receptor-mediated signaling in monocytes and dendritic cells from small samples of human blood.
Qian F, Montgomery RR. Quantitative imaging of lineage-specific Toll-like receptor-mediated signaling in monocytes and dendritic cells from small samples of human blood. Journal Of Visualized Experiments 2012 PMID: 22525943, PMCID: PMC3466655, DOI: 10.3791/3741.Peer-Reviewed Original ResearchConceptsToll-like receptorsDendritic cellsImmune responseToll-like receptor-mediated signalingCell-mediated immune responsesCertain Toll-like receptorsAntigen-presenting cellsPeripheral blood monocytesHealthy human donorsNF-κB pathwayInnate immune systemReceptor-mediated signalingQuantitative flow cytometryTLR levelsWest Nile virusPatient susceptibilityUnderstanding of immunosenescenceImmune responsivenessElderly donorsLarge cohortOlder donorsAdaptive immunityBlood monocytesNF-κBTherapeutic interventions
2011
Innate immune control of West Nile virus infection
Arjona A, Wang P, Montgomery RR, Fikrig E. Innate immune control of West Nile virus infection. Cellular Microbiology 2011, 13: 1648-1658. PMID: 21790942, PMCID: PMC3196381, DOI: 10.1111/j.1462-5822.2011.01649.x.Peer-Reviewed Original ResearchConceptsWest Nile virusWNV infectionAntiviral innate immune mechanismsLong-term neurologic sequelaeWest Nile virus infectionRe-emerging zoonotic pathogenInnate immune controlInnate immune mechanismsLife-threatening meningoencephalitisInnate immune systemNeurologic sequelaeImmune controlInflammatory mediatorsImmune mechanismsMammalian hostsVirus infectionCurrent evidenceViral infectionAntiviral effectorsImmune systemFlaviviridae familyAntiviral mechanismInfectionNile virusJAK-STATImpaired Interferon Signaling in Dendritic Cells From Older Donors Infected In Vitro With West Nile Virus
Qian F, Wang X, Zhang L, Lin A, Zhao H, Fikrig E, Montgomery RR. Impaired Interferon Signaling in Dendritic Cells From Older Donors Infected In Vitro With West Nile Virus. The Journal Of Infectious Diseases 2011, 203: 1415-1424. PMID: 21398396, PMCID: PMC3080893, DOI: 10.1093/infdis/jir048.Peer-Reviewed Original ResearchConceptsDendritic cellsWest Nile virusOlder donorsAntiviral responseToll-like receptor 3Initial antiviral responseLate-phase responseNile virusSignificant age-related differencesSignificant human morbidityType I IFNQuantified cytokinesRNA flavivirusAge-related differencesYoung donorsI IFNReceptor RIGViral infectionReceptor 3Human morbidityOlder populationCritical regulatory pathwaysInterferon SignalingNuclear translocationDefective regulation
2009
IL-10 Signaling Blockade Controls Murine West Nile Virus Infection
Bai F, Town T, Qian F, Wang P, Kamanaka M, Connolly TM, Gate D, Montgomery RR, Flavell RA, Fikrig E. IL-10 Signaling Blockade Controls Murine West Nile Virus Infection. PLOS Pathogens 2009, 5: e1000610. PMID: 19816558, PMCID: PMC2749443, DOI: 10.1371/journal.ppat.1000610.Peer-Reviewed Original ResearchConceptsIL-10 signalingIL-10WNV infectionWest Nile virusIL-10-deficient miceWest Nile virus infectionImportant cellular sourceSignificant human morbidityRNA flavivirusWNV pathogenesisInterleukin-10Antiviral cytokinesEtiologic rolePharmacologic blockadeDeficient miceT cellsVirus infectionPharmacologic meansTherapeutic strategiesViral infectionCellular sourceInfectionHuman morbidityNile virusMiceFusion Loop Peptide of the West Nile Virus Envelope Protein Is Essential for Pathogenesis and Is Recognized by a Therapeutic Cross-Reactive Human Monoclonal Antibody
Sultana H, Foellmer HG, Neelakanta G, Oliphant T, Engle M, Ledizet M, Krishnan MN, Bonafé N, Anthony KG, Marasco WA, Kaplan P, Montgomery RR, Diamond MS, Koski RA, Fikrig E. Fusion Loop Peptide of the West Nile Virus Envelope Protein Is Essential for Pathogenesis and Is Recognized by a Therapeutic Cross-Reactive Human Monoclonal Antibody. The Journal Of Immunology 2009, 183: 650-660. PMID: 19535627, PMCID: PMC3690769, DOI: 10.4049/jimmunol.0900093.Peer-Reviewed Original ResearchConceptsWest Nile virus envelope proteinWest Nile virusVirus envelope proteinDengue virusCross-reactive human monoclonal antibodiesBlood-brain barrier permeabilityEnvelope proteinWest Nile virus infectionNeutralization escape variantsNile virusWest Nile encephalitisNeutralization escape mutantsHuman monoclonal antibodyFatal neurological diseaseParental West Nile virusFusion loopEscape variantsInflammatory responseBarrier permeabilityLethal encephalitisMAb11Virus infectionHuman mAbsEscape mutantsNeurological diseases