2015
DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Rαlow and IL-7Rαhigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine
Shin MS, You S, Kang Y, Lee N, Yoo SA, Park K, Kang KS, Kim SH, Mohanty S, Shaw AC, Montgomery RR, Hwang D, Kang I. DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Rαlow and IL-7Rαhigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine. The Journal Of Immunology 2015, 195: 2861-2869. PMID: 26276874, PMCID: PMC4561204, DOI: 10.4049/jimmunol.1500877.Peer-Reviewed Original ResearchConceptsDNA methylationDifferential expressionGene expressionCellular traitsEpigenetic mechanismsGene promoterGenome-wide DNA methylationDistinct traitsDNA methylation statusIndividual gene expressionMigratory capacityAutocrine amplification loopImportant biological outcomesPotential biological implicationsMammalian cellsMethylationMethylation statusAmplification loopBiological outcomesTraitsBiological implicationsPromoterEffector memoryExpressionT cells
2012
Impaired Toll-Like Receptor 3-Mediated Immune Responses from Macrophages of Patients Chronically Infected with Hepatitis C Virus
Qian F, Bolen CR, Jing C, Wang X, Zheng W, Zhao H, Fikrig E, Bruce RD, Kleinstein SH, Montgomery RR. Impaired Toll-Like Receptor 3-Mediated Immune Responses from Macrophages of Patients Chronically Infected with Hepatitis C Virus. MSphere 2012, 20: 146-155. PMID: 23220997, PMCID: PMC3571267, DOI: 10.1128/cvi.00530-12.Peer-Reviewed Original ResearchMeSH KeywordsAdultFemaleGene ExpressionGenotypeHepacivirusHepatitis C, ChronicHumansInflammationInterferon-betaInterferonsInterleukinsLeukocytes, MononuclearMacrophagesMalePhosphorylationPolymorphism, Single NucleotideSignal TransductionSTAT1 Transcription FactorToll-Like Receptor 3Tumor Necrosis Factor-alphaViral LoadConceptsToll-like receptor 3Peripheral blood mononuclear cellsHepatitis C virusImmune responseHCV patientsC virusExpression of TLR3Clearance of HCVCommon chronic blood-borne infectionElevated innate immune responseImpaired toll-like receptorPrimary macrophagesHCV genotype 1Ongoing inflammatory responseMajority of patientsBlood-borne infectionsBlood mononuclear cellsToll-like receptorsIFN response genesPotential therapeutic approachInnate immune responseMacrophages of patientsElevated baseline expressionTLR3 pathwayViral clearance
2011
Dual effect of the macrophage migration inhibitory factor gene on the development and severity of human systemic lupus erythematosus
Sreih A, Ezzeddine R, Leng L, LaChance A, Yu G, Mizue Y, Subrahmanyan L, Pons‐Estel B, Abelson A, Gunnarsson I, Svenungsson E, Cavett J, Glenn S, Zhang L, Montgomery R, Perl A, Salmon J, Alarcón‐Riquelme M, Harley JB, Bucala R. Dual effect of the macrophage migration inhibitory factor gene on the development and severity of human systemic lupus erythematosus. Arthritis & Rheumatism 2011, 63: 3942-3951. PMID: 22127710, PMCID: PMC3228269, DOI: 10.1002/art.30624.Peer-Reviewed Original ResearchMeSH KeywordsAdultBlack or African AmericanCase-Control StudiesCross-Sectional StudiesFemaleGenetic Predisposition to DiseaseGenotypeHumansLupus Erythematosus, SystemicMacrophage Migration-Inhibitory FactorsMaleMiddle AgedPhenotypePolymorphism, Single NucleotideSeverity of Illness IndexTumor Necrosis Factor-alphaWhite PeopleConceptsSystemic lupus erythematosusMacrophage migration inhibitory factorMIF genotypeMIF productionLupus erythematosusImmunopathogenesis of SLEInflammatory end-organ damageCentral nervous system involvementCytokine macrophage migration inhibitory factorToll-like receptor 7Human systemic lupus erythematosusMacrophage migration inhibitory factor geneMIF plasma levelsPlasma MIF levelsEnd-organ damageNervous system involvementEnd-organ involvementAfrican American patientsMigration inhibitory factorMIF levelsProinflammatory propertiesPlasma levelsReceptor 7System involvementAmerican patientsAge‐associated elevation in TLR5 leads to increased inflammatory responses in the elderly
Qian F, Wang X, Zhang L, Chen S, Piecychna M, Allore H, Bockenstedt L, Malawista S, Bucala R, Shaw AC, Fikrig E, Montgomery RR. Age‐associated elevation in TLR5 leads to increased inflammatory responses in the elderly. Aging Cell 2011, 11: 104-110. PMID: 22023165, PMCID: PMC3257374, DOI: 10.1111/j.1474-9726.2011.00759.x.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAgingExtracellular Signal-Regulated MAP KinasesFemaleHumansInflammationInterleukin-8MaleMiddle AgedMonocytesMultivariate AnalysisNF-kappa BP38 Mitogen-Activated Protein KinasesPhosphorylationProtein TransportRNA, MessengerSignal TransductionToll-Like Receptor 5Tumor Necrosis Factor-alphaConceptsToll-like receptorsIL-8Multivariable mixed-effects modelsOlder individualsElevated IL-8Levels of TLR5Expression of TLR5Production of TNFAge-associated elevationAge-related decreaseDendritic cellsImmune responsivenessElderly donorsInflammatory responseImmune functionNF-κBTLR5Progressive declineMonocytesMixed effects modelsMAPK p38Significant increaseEffects modelAssociated increaseCritical mechanism
2010
Age-Associated Decrease in TLR Function in Primary Human Dendritic Cells Predicts Influenza Vaccine Response
Panda A, Qian F, Mohanty S, van Duin D, Newman FK, Zhang L, Chen S, Towle V, Belshe RB, Fikrig E, Allore HG, Montgomery RR, Shaw AC. Age-Associated Decrease in TLR Function in Primary Human Dendritic Cells Predicts Influenza Vaccine Response. The Journal Of Immunology 2010, 184: 2518-2527. PMID: 20100933, PMCID: PMC3867271, DOI: 10.4049/jimmunol.0901022.Peer-Reviewed Original ResearchMeSH KeywordsAdultAge FactorsAgedAged, 80 and overAntibodies, ViralCytokinesDendritic CellsFemaleFlow CytometryHumansInfluenza A Virus, H1N1 SubtypeInfluenza VaccinesInterleukin-12 Subunit p40Interleukin-6Linear ModelsMaleMiddle AgedReverse Transcriptase Polymerase Chain ReactionToll-Like ReceptorsTumor Necrosis Factor-alphaYoung AdultConceptsPrimary human dendritic cellsDendritic cellsHuman dendritic cellsMyeloid DCsPlasmacytoid DCsCytokine productionTLR functionTNF-alphaIntracellular cytokine productionPoor Ab responsesInfluenza vaccine responsesMyeloid dendritic cellsPlasmacytoid dendritic cellsYoung individualsIntracellular cytokine stainingIL-12 productionIFN-alpha productionTLR ligand stimulationTLR gene expressionInnate immune responseAge-Associated DecreaseTLR8 engagementInfluenza immunizationAge-associated effectsCytokine staining
2007
Age-Associated Defect in Human TLR-1/2 Function
van Duin D, Mohanty S, Thomas V, Ginter S, Montgomery RR, Fikrig E, Allore HG, Medzhitov R, Shaw AC. Age-Associated Defect in Human TLR-1/2 Function. The Journal Of Immunology 2007, 178: 970-975. PMID: 17202359, DOI: 10.4049/jimmunol.178.2.970.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAgingCell MembraneFemaleHumansInterleukin-6MaleMiddle AgedToll-Like Receptor 1Toll-Like Receptor 2Tumor Necrosis Factor-alphaConceptsTLR1 surface expressionCytokine productionTLR functionTNF-alphaSurface expressionMultivariable mixed-effects modelsOlder adultsImpaired vaccine responsesTLR2 surface expressionInfection-related morbidityPeripheral blood monocytesYears of ageVaccine responsesIL-6Aged miceBlood monocytesYoung controlsFlow cytometryMixed effects modelsAge categoriesTLR1/2TLREffects modelN-palmitoylAdults
2001
Murine Lyme Disease: No Evidence for Active Immune Down-Regulation in Resolving or Subclinical Infection
Montgomery R, Wang X, Malawista S. Murine Lyme Disease: No Evidence for Active Immune Down-Regulation in Resolving or Subclinical Infection. The Journal Of Infectious Diseases 2001, 183: 1631-1637. PMID: 11343212, DOI: 10.1086/320703.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBorrelia burgdorferi GroupDisease Models, AnimalFemaleFluorescent Antibody TechniqueInterleukin-1Interleukin-12Lyme DiseaseMacrophage ActivationMacrophagesMiceMyocarditisPeritoneumReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSpecific Pathogen-Free OrganismsTumor Necrosis Factor-alphaConceptsActive diseaseAnti-inflammatory cytokine IL-10Proinflammatory cytokines IL-1Levels of proinflammatoryAnti-inflammatory cytokinesCytokine IL-10Double-label immunofluorescent stainingCytokines IL-1Reverse transcription-polymerase chain reaction analysisSemiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysisTranscription-polymerase chain reaction analysisInfected heartsIL-10IL-12Inflammatory activityCytokine expressionTumor necrosisLocal macrophagesIL-1Peritoneal cavitySubclinical infectionChain reaction analysisLevels of mRNAMacrophage activationImmune system
1989
Endocytic and Secretory Repertoire of the Lipid-Loaded Macrophage
Montgomery R, Cohn Z. Endocytic and Secretory Repertoire of the Lipid-Loaded Macrophage. Journal Of Leukocyte Biology 1989, 45: 129-138. PMID: 2492592, DOI: 10.1002/jlb.45.2.129.Peer-Reviewed Original ResearchConceptsLipid-laden cellsOil Red O stainingTumor necrosis factorArachidonic acid metabolitesRed O stainingIntracellular lipid poolsWestern blot analysisIntracellular lipid storageMouse peritoneal macrophagesCholesterol levelsNecrosis factorMacrophage functionO stainingScavenger receptorsSecretory functionAcid metabolitesAtherosclerotic arteriesPeritoneal macrophagesFunctional changesFibrinolytic activitySheep erythrocytesCholesterol acceptorsMacrophagesSecretory productsLipid storage