2019
BOK promotes erythropoiesis in a mouse model of myelodysplastic syndrome
Kang SH, Perales O, Michaud M, Katz SG. BOK promotes erythropoiesis in a mouse model of myelodysplastic syndrome. Annals Of Hematology 2019, 98: 2089-2096. PMID: 31203423, PMCID: PMC6702064, DOI: 10.1007/s00277-019-03726-7.Peer-Reviewed Original ResearchConceptsUnfolded protein responseER stressPro-apoptotic membersBcl-2 familyNHD13 miceRT-qPCR analysisInduction of apoptosisProgenitor stem cellsHematopoietic progenitor cell assaysProtein responseDownstream effectorsGene knockoutMyelodysplastic syndromeCell stressProgenitor cell assaysEndoplasmic reticulumLower mean cell hemoglobin concentrationErythroid progenitorsNUP98-HOXD13 transgenic miceClonal hematopoietic stem cell disordersStem cellsSimilar overall survivalAcute myeloid leukemiaHematopoietic stem cell disordersMean cell hemoglobin concentration
2015
BCL-2 family member BOK promotes apoptosis in response to endoplasmic reticulum stress
Carpio MA, Michaud M, Zhou W, Fisher JK, Walensky LD, Katz SG. BCL-2 family member BOK promotes apoptosis in response to endoplasmic reticulum stress. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 7201-7206. PMID: 26015568, PMCID: PMC4466744, DOI: 10.1073/pnas.1421063112.Peer-Reviewed Original ResearchConceptsB-cell lymphoma 2 ovarian killerApoptotic defectsMultidomain proapoptotic proteins BaxApoptotic responseStress stimuliER stressBcl-2 family proteinsER stress agentsUnfolded protein responseMouse embryonic fibroblastsDefective apoptotic responseMitochondrial apoptotic pathwayProapoptotic protein BaxPredominant subcellular localizationThapsigargin-induced apoptosisEndoplasmic reticulum stressFamily proteinsDeath responseSubcellular localizationEmbryonic fibroblastsHigh homologyProtein responseApoptotic pathwayOvert phenotypeProtein Bax
2013
Mantle cell lymphoma in cyclin D1 transgenic mice with Bim-deficient B cells
Katz SG, LaBelle JL, Meng H, Valeriano RP, Fisher JK, Sun H, Rodig SJ, Kleinstein SH, Walensky LD. Mantle cell lymphoma in cyclin D1 transgenic mice with Bim-deficient B cells. Blood 2013, 123: 884-893. PMID: 24352880, PMCID: PMC3916879, DOI: 10.1182/blood-2013-04-499079.Peer-Reviewed Original ResearchConceptsMantle cell lymphomaCyclin D1 transgenic miceCyclin D1 overexpressionB cellsCell lymphomaAggressive B-cell lymphomasSubset of miceTransgenic mouse modelB-cell lymphomaDeletion of BimPathogenesis of MCLHuman mantle cell lymphomaDevelopment of MCLStimulation regimensConventional chemotherapyMouse modelLymphoid maturationTransgenic miceLymphomaBIM deletionSelective expansionMiceProapoptotic BimPathogenesisGenetic aberrations
2012
Brain and testicular tumors in mice with progenitor cells lacking BAX and BAK
Katz SG, Fisher JK, Correll M, Bronson RT, Ligon KL, Walensky LD. Brain and testicular tumors in mice with progenitor cells lacking BAX and BAK. Oncogene 2012, 32: 4078-4085. PMID: 22986529, PMCID: PMC3529761, DOI: 10.1038/onc.2012.421.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsProapoptotic Bcl-2 family proteins BaxSubventricular zoneBcl-2 family proteins BaxProgenitor cellsLong-term pathophysiological consequencesMitochondrial outer membraneBax/BakDeletion of BaxStem cell nicheHigh-grade tumorsMultiple developmental abnormalitiesIntrinsic apoptotic pathwayOlfactory bulb regionNestin-positive cellsProgenitor cell poolCellular excessDeath stimuliLoss of functionHypercellular tumorOuter membraneTesticular tumorsCell nicheLong-term regulationApoptotic pathwayA stapled BIM peptide overcomes apoptotic resistance in hematologic cancers
LaBelle JL, Katz SG, Bird GH, Gavathiotis E, Stewart ML, Lawrence C, Fisher JK, Godes M, Pitter K, Kung AL, Walensky LD. A stapled BIM peptide overcomes apoptotic resistance in hematologic cancers. Journal Of Clinical Investigation 2012, 122: 2018-2031. PMID: 22622039, PMCID: PMC3366394, DOI: 10.1172/jci46231.Peer-Reviewed Original ResearchConceptsBcl-2 family proteinsCell deathFamily proteinsPro-apoptotic Bcl-2 family proteinsBcl-2 protein familyBcl-2-interacting mediatorBcl-2 family pathwayHydrocarbon-stapled peptidesCell death mechanismsCancer cellsCellular lifeHematologic cancer cellsProtein familyDeath domainBH3 helixBim BH3Bim peptidesDeath mechanismsApoptotic blockadesApoptotic resistanceFamily pathwaySurvival pathwaysAntiapoptotic proteinsProapoptotic activityHuman AML xenograft model
2008
BAX activation is initiated at a novel interaction site
Gavathiotis E, Suzuki M, Davis ML, Pitter K, Bird GH, Katz SG, Tu HC, Kim H, Cheng E, Tjandra N, Walensky LD. BAX activation is initiated at a novel interaction site. Nature 2008, 455: 1076-1081. PMID: 18948948, PMCID: PMC2597110, DOI: 10.1038/nature07396.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsApoptosisApoptosis Regulatory ProteinsBcl-2-Associated X ProteinBcl-2-Like Protein 11BH3 Interacting Domain Death Agonist ProteinCell LineGene Expression RegulationHumansMembrane ProteinsMiceMutagenesis, Site-DirectedMutationNuclear Magnetic Resonance, BiomolecularProtein BindingProto-Oncogene ProteinsSequence AlignmentConceptsAnti-apoptotic proteinsInteraction sitesBax activationBax-mediated cell deathBCL-2 domainsCell deathBcl-2 familyNovel interaction sitePro-apoptotic proteinsPoint mutagenesisMitochondrial apoptosisBax interactionStress stimuliΑ-helixProteinNew targetsBaxTherapeutic modulationDirect activationActivation siteApoptosisActivationFunctional activitySitesMutagenesisGATA4 mediates gene repression in the mature mouse small intestine through interactions with friend of GATA (FOG) cofactors
Beuling E, Bosse T, de Kerk D, Piaseckyj CM, Fujiwara Y, Katz SG, Orkin SH, Grand RJ, Krasinski SD. GATA4 mediates gene repression in the mature mouse small intestine through interactions with friend of GATA (FOG) cofactors. Developmental Biology 2008, 322: 179-189. PMID: 18692040, PMCID: PMC3031907, DOI: 10.1016/j.ydbio.2008.07.022.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCell DifferentiationCell ProliferationEnterocytesGATA4 Transcription FactorGene Expression RegulationIntestinal AbsorptionIntestinal MucosaIntestine, SmallMiceMice, TransgenicNuclear ProteinsOrgan SpecificityOrganic Anion Transporters, Sodium-DependentRNA, MessengerSymportersTranscription FactorsTranscriptional ActivationConceptsFriend of GATAGene repressionCell fate determinationFamily of cofactorsMammalian small intestineProximal-distal patternRepression functionGata (FOG) cofactorsFate determinationCell lineage markersFOG cofactorsGATA factorsGATA4 mutantsGene activationTranscription factorsGATA4 functionIntestinal epithelial cellsGATA4Multiple tissuesRepressionLineage markersMouse small intestineCofactorEpithelial cellsGenes
2003
Endothelial lineage-mediated loss of the GATA cofactor Friend of GATA 1 impairs cardiac development
Katz SG, Williams A, Yang J, Fujiwara Y, Tsang AP, Epstein JA, Orkin SH. Endothelial lineage-mediated loss of the GATA cofactor Friend of GATA 1 impairs cardiac development. Proceedings Of The National Academy Of Sciences Of The United States Of America 2003, 100: 14030-14035. PMID: 14614148, PMCID: PMC283540, DOI: 10.1073/pnas.1936250100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsDNA-Binding ProteinsEndotheliumErythroid-Specific DNA-Binding FactorsFetal HeartGATA1 Transcription FactorHeart Defects, CongenitalHeart ValvesHematopoiesisIn Situ HybridizationMiceMice, Inbred C57BLMice, KnockoutMice, TransgenicNeural CrestNuclear ProteinsTranscription FactorsConceptsFOG-1GATA-4/5/6FOG-2Cardiac developmentConditional inactivationGATA transcription factorsDiverse cell typesNeural crest cellsNeural crest derivativesGata (FOG) cofactorsEmbryonic day 14.5Cofactor FriendTranscription factorsHematopoietic defectsHeart developmentCrest cellsTransgenic rescueCrest derivativesUnsuspected roleGATA-4Nonredundant requirementCell typesCell of originDay 14.5Atrioventricular valve
2002
Interaction between FOG-1 and the Corepressor C-Terminal Binding Protein Is Dispensable for Normal Erythropoiesis In Vivo
Katz SG, Cantor AB, Orkin SH. Interaction between FOG-1 and the Corepressor C-Terminal Binding Protein Is Dispensable for Normal Erythropoiesis In Vivo. Molecular And Cellular Biology 2002, 22: 3121-3128. PMID: 11940669, PMCID: PMC133767, DOI: 10.1128/mcb.22.9.3121-3128.2002.Peer-Reviewed Original ResearchMeSH KeywordsAlcohol OxidoreductasesAmino Acid MotifsAmino Acid SequenceAnimalsBinding SitesBlotting, WesternCarrier ProteinsCell LineConserved SequenceCOS CellsDNA-Binding ProteinsErythrocytesErythropoiesisErythropoietinGenetic VectorsHematocritMiceMice, KnockoutMutationNuclear ProteinsPhenylhydrazinesPhosphoproteinsPrecipitin TestsProtein BindingRepressor ProteinsSequence Homology, Amino AcidTranscription FactorsTransgenesConceptsC-terminal binding proteinFOG-1Corepressor C-terminal binding proteinTranscription factor GATA-1Binding proteinCorepressor CtBPCtBP interactsErythroid developmentGATA-1Vivo functionCtBPMegakaryocytic lineagePeptide motifsPhysiological roleInteraction sitesNormal erythropoiesisErythropoietic stressCell linesStages of developmentProteinErythrocyte productionFamily membersWild-type miceErythropoiesisCoimmunoprecipitation
2001
Friend of GATA-1 Represses GATA-3–dependent Activity in CD4+ T Cells
Zhou M, Ouyang W, Gong Q, Katz S, White J, Orkin S, Murphy K. Friend of GATA-1 Represses GATA-3–dependent Activity in CD4+ T Cells. Journal Of Experimental Medicine 2001, 194: 1461-1471. PMID: 11714753, PMCID: PMC2193678, DOI: 10.1084/jem.194.10.1461.Peer-Reviewed Original ResearchConceptsFOG-1Friend of GATATranscription factor activityActivator of transcriptionIL-5 promoterHematopoietic developmentMegakaryocyte differentiationSignal transducerGATA-3Dependent inductionFactor activityNaive T cellsTh2 developmentT cellsOverexpressionAutoactivationCellsGATA-3 expressionPrimary activationActivationInductionTranscriptionPromoterGATAProtein