2015
Next-generation sequencing is highly sensitive for the detection of beta-catenin mutations in desmoid-type fibromatoses
Aitken S, Presneau N, Kalimuthu S, Dileo P, Berisha F, Tirabosco R, Amary M, Flanagan A. Next-generation sequencing is highly sensitive for the detection of beta-catenin mutations in desmoid-type fibromatoses. Virchows Archiv 2015, 467: 203-210. PMID: 25838078, DOI: 10.1007/s00428-015-1765-0.Peer-Reviewed Original ResearchConceptsNext-generation sequencingRestriction enzyme digestionMutation-specific restriction enzyme digestionEnzyme digestionIon Torrent Personal Genome MachineDesmoid-type fibromatosesPersonal Genome MachineMutation detection techniquesSpecificity of next-generation sequencingPolymerase chain reaction amplificationPrimer pairsCTNNB1 mutationsMinimal DNAMutational hotspotsBeta-catenin mutationsDetected CTNNB1 mutationsBeta-cateninMutationsDNAParaffin-embedded needle biopsiesSequenceSpindle cell lesionsMultiplex assayRecurrent tumorsNeedle biopsy
2008
β2-Adrenoreceptor ligands regulate osteoclast differentiation in vitro by direct and indirect mechanisms
Aitken S, Landao-Bassonga E, Ralston S, Idris A. β2-Adrenoreceptor ligands regulate osteoclast differentiation in vitro by direct and indirect mechanisms. Archives Of Biochemistry And Biophysics 2008, 482: 96-103. PMID: 19059194, DOI: 10.1016/j.abb.2008.11.012.Peer-Reviewed Original ResearchConceptsExpression of RANK-LBeta2-adrenoceptorOsteoclast formationBeta-adrenoceptorIncreased expression of RANK-LBone resorptionInfluence bone mineral densityOsteoclast differentiation in vitroRegulation of bone massBone mineral densityEffect of noradrenalineRANK-LSympathetic nervous systemInduce osteoclast formationOsteoblasts co-culturedDifferentiation in vitroAgonist isoprenalineMineral densityBeta3-adrenoceptorFracture riskLevels of beta1Beta-adrenoreceptorsBone massPharmacological agonistsOsteoblast growth