2021
Epor Stimulates Rapid Cycling and Larger Red Cells during Mouse and Human Erythropoiesis
Hidalgo D, Bejder J, Pop R, Gellatly K, Hwang Y, Scalf S, Eastman A, Chen J, Zhu L, Heuberger J, Guo S, Koury M, Nordsborg N, Socolovsky M. Epor Stimulates Rapid Cycling and Larger Red Cells during Mouse and Human Erythropoiesis. Blood 2021, 138: 852. DOI: 10.1182/blood-2021-154403.Peer-Reviewed Original ResearchErythroid terminal differentiationCell divisionCell cycleWild-type erythroblastsAnti-apoptotic protein BclCell sizeLive-cell reporterCell cycle speedNon-redundant functionsLarger red cellsFetal liver progenitorsTransferrin receptorEIF2α kinaseEpoR functionErythroblast survivalGenetic systemProtein BclHuman erythropoiesisNegative regulatorSurvival signalsTerminal differentiationEffects of EpoEpoRMouse EpoRP27 Kip1
2020
Novel Fluorescent Timer Tool Enables Characterization of Erythropoietic Differentiation Based on Differential Cell Cycling Speeds
Modepalli S, Eastman A, Shaw C, Guo S, Hattangadi S, Kupfer G. Novel Fluorescent Timer Tool Enables Characterization of Erythropoietic Differentiation Based on Differential Cell Cycling Speeds. Blood 2020, 136: 27-28. DOI: 10.1182/blood-2020-141666.Peer-Reviewed Original ResearchCell cycle speedCell divisionErythroid differentiationFusion proteinOrthochromatic erythroblastsLive-cell reporterStress erythropoiesisFluorescent timer proteinsAvailable proteomic dataSubsequent cell divisionRapid cell divisionUpregulation of genesCell cycle dynamicsErythroid cell proliferationSingle-cell levelRed fluorescent proteinFaster cycling cellsCell cycle behaviorFT proteinPhosphoproteomic investigationsDiscrete molecular targetsInducible promoterProteomic dataActive lociStress hematopoiesis2014 – FLUORESCENT CELL CYCLE TIMER ENABLED ANALYSIS OF NORMAL AND INEFFECTIVE ERYTHROPOIESIS
Modepalli S, Eastman A, Shaw C, Guo S, Hattangadi S, Kupfer G. 2014 – FLUORESCENT CELL CYCLE TIMER ENABLED ANALYSIS OF NORMAL AND INEFFECTIVE ERYTHROPOIESIS. Experimental Hematology 2020, 88: s32. DOI: 10.1016/j.exphem.2020.09.176.Peer-Reviewed Original ResearchCell cycle speedCell cycleLive-cell reporterFluorescent timer proteinsErythropoietic responseIneffective erythropoiesisFaster cycling cellsErythroblast stageFlow cytometric sortingStress hematopoiesisDiamond-Blackfan anemiaTimer proteinFusion proteinCellular factorsEarly progenitorsCell stagePrimary cell culturesSorted populationsCell cycling ratesMetabolic pathwaysCycling cellsStress erythropoiesisHuman CD34Cytometric sortingIntracellular ratio