2019
In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens
Chow RD, Wang G, Ye L, Codina A, Kim HR, Shen L, Dong MB, Errami Y, Chen S. In vivo profiling of metastatic double knockouts through CRISPR–Cpf1 screens. Nature Methods 2019, 16: 405-408. PMID: 30962622, PMCID: PMC6592845, DOI: 10.1038/s41592-019-0371-5.Peer-Reviewed Original Research
2017
AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma
Chow RD, Guzman CD, Wang G, Schmidt F, Youngblood MW, Ye L, Errami Y, Dong MB, Martinez MA, Zhang S, Renauer P, Bilguvar K, Gunel M, Sharp PA, Zhang F, Platt RJ, Chen S. AAV-mediated direct in vivo CRISPR screen identifies functional suppressors in glioblastoma. Nature Neuroscience 2017, 20: 1329-1341. PMID: 28805815, PMCID: PMC5614841, DOI: 10.1038/nn.4620.Peer-Reviewed Original Research
2015
Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis
Chen S, Sanjana NE, Zheng K, Shalem O, Lee K, Shi X, Scott DA, Song J, Pan JQ, Weissleder R, Lee H, Zhang F, Sharp PA. Genome-wide CRISPR Screen in a Mouse Model of Tumor Growth and Metastasis. Cell 2015, 160: 1246-1260. PMID: 25748654, PMCID: PMC4380877, DOI: 10.1016/j.cell.2015.02.038.Peer-Reviewed Original ResearchConceptsSingle guide RNAsGenome-wide CRISPR screenGenome-scale libraryCRISPR/Cas9-mediated lossLate-stage primary tumorsEffects of mutationsGenetic screenTumor growthCRISPR screensFunction screenMouse cancer cell linesCancer evolutionGene phenotypeDiverse phenotypesFunction mutationsCancer cell linesGenesCell linesPrimary screenCell poolSpecific lossPrimary tumor growthSmall poolMutationsPhenotype
2014
Global microRNA depletion suppresses tumor angiogenesis
Chen S, Xue Y, Wu X, Le C, Bhutkar A, Bell EL, Zhang F, Langer R, Sharp PA. Global microRNA depletion suppresses tumor angiogenesis. Genes & Development 2014, 28: 1054-1067. PMID: 24788094, PMCID: PMC4035535, DOI: 10.1101/gad.239681.114.Peer-Reviewed Original ResearchConceptsUntranslated regionCRISPR/Multiplexed CRISPR/HIF transcriptional activityHypoxia-inducible factor-1Tumor angiogenesisMicroRNA-binding sitesGenome engineeringExpression profilingTranscriptional activityHypoxia responseBalance of angiogenesisHIF transcriptionMicroRNA deficiencyMicroRNAsFIH1Factor 1Angiogenesis genesDeficient angiogenesisAngiogenesisVEGF productionTranscription