Endosomal regulation of contact inhibition through the AMOT:YAP pathway
Cox CM, Mandell EK, Stewart L, Lu R, Johnson DL, McCarter SD, Tavares A, Runyan R, Ghosh S, Wilson JM. Endosomal regulation of contact inhibition through the AMOT:YAP pathway. Molecular Biology Of The Cell 2015, 26: 2673-2684. PMID: 25995376, PMCID: PMC4501364, DOI: 10.1091/mbc.e15-04-0224.Peer-Reviewed Original ResearchConceptsEndosomal membranesYAP activitySubconfluent cellsOrgan growth controlTranscription coactivator YesPhosphorylation-dependent regulationIntegral membrane proteinsConfluent cellsAmot proteinsEndosomal regulationContact-mediated inhibitionCytoplasmic domainEndotubinMembrane proteinsOvergrowth phenotypeInduces translocationAngiomotinYAP interactionDirect bindingGrowth controlContact inhibitionFamily membersCell growthYAPYAP pathwayPKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels
Lu R, Dalgalan D, Mandell EK, Parker SS, Ghosh S, Wilson JM. PKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels. Molecular Biology Of The Cell 2015, 26: 1523-1531. PMID: 25694446, PMCID: PMC4395131, DOI: 10.1091/mbc.e14-12-1613.Peer-Reviewed Original ResearchConceptsClaudin-2 levelsSmall GTPase Rab14Claudin-2 protein levelsClaudin-2Junction proteinsTransepithelial resistanceEpithelial polarityIntracellular punctaTight junction componentsTight junction proteinsPlasma membraneNormal assemblyRab14 expressionRab14Junction componentsPKCιKnockdownEpithelial barrier functionProtein levelsTight junctionsZO-1ProteinParacellular permeabilityClaudin-1APKC