2021
Small RNAs are modified with N-glycans and displayed on the surface of living cells
Flynn RA, Pedram K, Malaker SA, Batista PJ, Smith BAH, Johnson AG, George BM, Majzoub K, Villalta PW, Carette JE, Bertozzi CR. Small RNAs are modified with N-glycans and displayed on the surface of living cells. Cell 2021, 184: 3109-3124.e22. PMID: 34004145, PMCID: PMC9097497, DOI: 10.1016/j.cell.2021.04.023.Peer-Reviewed Original ResearchConceptsLiving cellsGlycan biosynthetic machineryDomains of lifeMultiple cell typesRNA biologySmall RNAsExtracellular biologyBiosynthetic machineryBiochemical approachesMammalian speciesBattery of chemicalAnti-dsRNA antibodiesN-glycansCell typesReceptor familyCultured cellsCell surfaceRNAThird scaffoldGlycoRNABiologyMajor targetGlycosylationGlycansSialic acid
2020
Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors
Rumachik NG, Malaker SA, Poweleit N, Maynard LH, Adams CM, Leib RD, Cirolia G, Thomas D, Stamnes S, Holt K, Sinn P, May AP, Paulk NK. Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors. Molecular Therapy — Methods & Clinical Development 2020, 18: 98-118. PMID: 32995354, PMCID: PMC7488757, DOI: 10.1016/j.omtm.2020.05.018.Peer-Reviewed Original ResearchPost-translational modificationsHost cell speciesHuman HEK293 cellsComparative production analysisEpigenomic sequencingInsect cellsHuman cytokine profilingProteomic profilingMultiple analytical approachesHEK293 cellsExpression kineticsMouse tissuesCell typesCell speciesHost cell protein impuritiesRAAV genomesDenaturation assaysGenomeProduction platformMajor discoveriesProfilingRAAV capsidsMass spectrometryReceptor bindingCells