2024
Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome
Mahoney K, Chang V, Lucas T, Maruszko K, Malaker S. Mass Spectrometry-Compatible Elution Technique Enables an Improved Mucin-Selective Enrichment Strategy to Probe the Mucinome. Analytical Chemistry 2024, 96: 5242-5250. PMID: 38512228, DOI: 10.1021/acs.analchem.3c05762.Peer-Reviewed Original ResearchMucin-domain glycoproteinsO-glycopeptidesGlycopeptide signalsIn-gel digestionMass spectrometryElution conditionsSolid supportBinding moietyElution stepDownstream analysisO-glycosylationIn-gelBiological functionsElutionHuman serumEnrichment strategyCell linesMoietyEffective isolationSpectrometryCatalyticallyGlycoproteinSampling requirementsGlycopeptidesStcE
2019
Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification
Penny SA, Malaker SA. Isolation of Major Histocompatibility Complex (MHC)-Associated Peptides by Immunoaffinity Purification. Methods In Molecular Biology 2019, 2024: 235-243. PMID: 31364053, DOI: 10.1007/978-1-4939-9597-4_14.ChaptersConceptsMass spectrometryAntibody conjugationImmunoaffinity purificationSurface of cellsMajor histocompatibility complexAutoimmune diseasesT cellsImmune componentsDisease processMoleculesHistocompatibility complexAntigenic peptidesMHCAssociated peptidePurificationPeptidesSurfaceSpectrometryElutionCellsComplexesConjugationCD8CD4Cancer