Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors
Rumachik NG, Malaker SA, Poweleit N, Maynard LH, Adams CM, Leib RD, Cirolia G, Thomas D, Stamnes S, Holt K, Sinn P, May AP, Paulk NK. Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors. Molecular Therapy — Methods & Clinical Development 2020, 18: 98-118. PMID: 32995354, PMCID: PMC7488757, DOI: 10.1016/j.omtm.2020.05.018.Peer-Reviewed Original ResearchPost-translational modificationsHost cell speciesHuman HEK293 cellsComparative production analysisEpigenomic sequencingInsect cellsHuman cytokine profilingProteomic profilingMultiple analytical approachesHEK293 cellsExpression kineticsMouse tissuesCell typesCell speciesHost cell protein impuritiesRAAV genomesDenaturation assaysGenomeProduction platformMajor discoveriesProfilingRAAV capsidsMass spectrometryReceptor bindingCells