2022
A microbial transporter of the dietary antioxidant ergothioneine
Dumitrescu D, Gordon E, Kovalyova Y, Seminara A, Duncan-Lowey B, Forster E, Zhou W, Booth C, Shen A, Kranzusch P, Hatzios S. A microbial transporter of the dietary antioxidant ergothioneine. Cell 2022, 185: 4526-4540.e18. PMID: 36347253, PMCID: PMC9691600, DOI: 10.1016/j.cell.2022.10.008.Peer-Reviewed Original ResearchConceptsInter-kingdom competitionHost-associated microbesIntracellular redox homeostasisGastric pathogen Helicobacter pyloriPathogen Helicobacter pyloriRedox regulationSmall molecule antioxidantsRedox homeostasisBiosynthetic pathwayColonization advantageUnappreciated mechanismLMW thiolsHost environmentHuman faecal bacteriaWeight thiolsCertain microorganismsAntioxidant ergothioneineGastrointestinal microbesMetabolite trimethylamine N-oxideMicrobesMillimolar levelsHuman tissuesErgothioneineTrimethylamine N-oxideFecal bacteriaAn infection-induced oxidation site regulates legumain processing and tumor growth
Kovalyova Y, Bak DW, Gordon EM, Fung C, Shuman JHB, Cover TL, Amieva MR, Weerapana E, Hatzios SK. An infection-induced oxidation site regulates legumain processing and tumor growth. Nature Chemical Biology 2022, 18: 698-705. PMID: 35332331, PMCID: PMC9246868, DOI: 10.1038/s41589-022-00992-x.Peer-Reviewed Original ResearchConceptsCysteine reactivityOxidation sitesTumor growthChemical proteomicsH. pylori-infected cellsRedox-sensitive cysteinesInduces oxidationReactivityOxidationOxidation EventHuman gastric cellsCarcinogenic infectionsBacterium Helicobacter pyloriXenograft modelHelicobacter pyloriSite-specific lossGastric cellsMost cancersOxidative stressReactive oxygen speciesTumor formationOxygen speciesInfectionTumorigenesisCysteine
2020
Chemical tools for decoding redox signaling at the host–microbe interface
Gordon EM, Hatzios SK. Chemical tools for decoding redox signaling at the host–microbe interface. PLOS Pathogens 2020, 16: e1009070. PMID: 33332456, PMCID: PMC7745962, DOI: 10.1371/journal.ppat.1009070.Peer-Reviewed Original Research
2019
Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
Howell M, Dumitrescu DG, Blankenship LR, Herkert D, Hatzios SK. Functional characterization of a subtilisin-like serine protease from Vibrio cholerae. Journal Of Biological Chemistry 2019, 294: 9888-9900. PMID: 31076508, PMCID: PMC6597830, DOI: 10.1074/jbc.ra119.007745.Peer-Reviewed Original ResearchConceptsSubtilisin-like enzymesSerine proteasesSubtilisin-like serine proteaseProtein domain structureDiarrheal disease choleraSerine hydrolase activityNutrient acquisitionAquatic nichesCarbohydrate-binding proteinsPathogen enzymesMature enzymeReporter proteinBiofilm assemblyFunctional characterizationNumerous enzymesDisease choleraCleavage eventsIntelectinLarge familyStepwise processingVibrio choleraeCleavage patternsEnzymeProteaseHydrolase activity
2017
Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease
Stockwell BR, Angeli J, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascón S, Hatzios SK, Kagan VE, Noel K, Jiang X, Linkermann A, Murphy ME, Overholtzer M, Oyagi A, Pagnussat GC, Park J, Ran Q, Rosenfeld CS, Salnikow K, Tang D, Torti FM, Torti SV, Toyokuni S, Woerpel KA, Zhang DD. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. Cell 2017, 171: 273-285. PMID: 28985560, PMCID: PMC5685180, DOI: 10.1016/j.cell.2017.09.021.Peer-Reviewed Original ResearchConceptsRegulated cell deathCell deathPathological cell deathBiosynthesis of glutathioneNumerous biological processesTumor suppressor functionIron-dependent accumulationAreas of biologyRedox biologyFatty acid metabolismBiological processesCellular membranesAmino acidsHeat stressCoenzyme QFerroptosisAcid metabolismBiologyLethal levelsFatty acidsKidney degenerationCancer therapyDegenerative diseasesMetabolismMammals
2016
Chemoproteomic profiling of host and pathogen enzymes active in cholera
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK. Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nature Chemical Biology 2016, 12: 268-274. PMID: 26900865, PMCID: PMC4765928, DOI: 10.1038/nchembio.2025.Peer-Reviewed Original Research