2023
Isotope tracing reveals bacterial catabolism of host-derived glutathione during Helicobacter pylori infection
Baskerville M, Kovalyova Y, Mejías-Luque R, Gerhard M, Hatzios S. Isotope tracing reveals bacterial catabolism of host-derived glutathione during Helicobacter pylori infection. PLOS Pathogens 2023, 19: e1011526. PMID: 37494402, PMCID: PMC10406306, DOI: 10.1371/journal.ppat.1011526.Peer-Reviewed Original ResearchConceptsH. pylori infectionPylori infectionInfected cellsBacterial catabolismGastric pathogen Helicobacter pyloriGSH depletionPathogen Helicobacter pyloriH. pylori-infected cellsMammalian cellsReactive oxygen speciesHelicobacter pylori infectionH. pylori colonizationCellular biomoleculesGSH homeostasisBacterial acquisitionCertain bacteriaVirulence factorsAntioxidant glutathioneIntracellular GSHOxidative damageOxidized GSHΓ-glutamyl transpeptidaseIsotope tracingHost tissuesOxygen species
2019
Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
Howell M, Dumitrescu DG, Blankenship LR, Herkert D, Hatzios SK. Functional characterization of a subtilisin-like serine protease from Vibrio cholerae. Journal Of Biological Chemistry 2019, 294: 9888-9900. PMID: 31076508, PMCID: PMC6597830, DOI: 10.1074/jbc.ra119.007745.Peer-Reviewed Original ResearchConceptsSubtilisin-like enzymesSerine proteasesSubtilisin-like serine proteaseProtein domain structureDiarrheal disease choleraSerine hydrolase activityNutrient acquisitionAquatic nichesCarbohydrate-binding proteinsPathogen enzymesMature enzymeReporter proteinBiofilm assemblyFunctional characterizationNumerous enzymesDisease choleraCleavage eventsIntelectinLarge familyStepwise processingVibrio choleraeCleavage patternsEnzymeProteaseHydrolase activity
2018
Activity-Based Protein Profiling at the Host–Pathogen Interface
Kovalyova Y, Hatzios SK. Activity-Based Protein Profiling at the Host–Pathogen Interface. Current Topics In Microbiology And Immunology 2018, 420: 73-91. PMID: 30203396, DOI: 10.1007/82_2018_129.BooksConceptsActivity-based protein profilingHost-pathogen interfaceProtein profilingFunctional proteomePathogen interactionsMicrobial pathogenicityComplex proteomesEnzyme-mediated mechanismReactive amino acidsActive enzymeChemical probesAmino acidsMetabolic adaptationProteomeMicrobial infectionsCo-culture systemBiological systemsHost immunityEnzymeProfilingPathogenicityHostProbeAnimal modelsAdaptation
2017
Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease
Stockwell BR, Angeli J, Bayir H, Bush AI, Conrad M, Dixon SJ, Fulda S, Gascón S, Hatzios SK, Kagan VE, Noel K, Jiang X, Linkermann A, Murphy ME, Overholtzer M, Oyagi A, Pagnussat GC, Park J, Ran Q, Rosenfeld CS, Salnikow K, Tang D, Torti FM, Torti SV, Toyokuni S, Woerpel KA, Zhang DD. Ferroptosis: A Regulated Cell Death Nexus Linking Metabolism, Redox Biology, and Disease. Cell 2017, 171: 273-285. PMID: 28985560, PMCID: PMC5685180, DOI: 10.1016/j.cell.2017.09.021.Peer-Reviewed Original ResearchConceptsRegulated cell deathCell deathPathological cell deathBiosynthesis of glutathioneNumerous biological processesTumor suppressor functionIron-dependent accumulationAreas of biologyRedox biologyFatty acid metabolismBiological processesCellular membranesAmino acidsHeat stressCoenzyme QFerroptosisAcid metabolismBiologyLethal levelsFatty acidsKidney degenerationCancer therapyDegenerative diseasesMetabolismMammals
2016
Chemoproteomic profiling of host and pathogen enzymes active in cholera
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK. Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nature Chemical Biology 2016, 12: 268-274. PMID: 26900865, PMCID: PMC4765928, DOI: 10.1038/nchembio.2025.Peer-Reviewed Original Research