2015
A protein interaction map of the LSU processome
McCann KL, Charette JM, Vincent NG, Baserga SJ. A protein interaction map of the LSU processome. Genes & Development 2015, 29: 862-875. PMID: 25877921, PMCID: PMC4403261, DOI: 10.1101/gad.256370.114.Peer-Reviewed Original ResearchConceptsLarge ribosomal subunitBiogenesis factorsRibosome biogenesis factorsTwo-hybrid assayProtein interaction mapsProtein-protein interactionsSystems biology approachInteractome mapHub proteinsBiology approachProcessomeProtein pairsRibosomal subunitInteraction mapCoordinated processConcerted actionCurrent knowledgeEukaryotesNOP4SubcomplexSubunitsProteinFourfold increaseMaturationAssembly
2014
Making Ribosomes: Pre-rRNA Transcription and Processing
McCann K, Baserga S. Making Ribosomes: Pre-rRNA Transcription and Processing. 2014, 217-232. DOI: 10.1007/978-3-319-05687-6_9.Peer-Reviewed Original ResearchDiscovering the pre‐60S ribosome biogenesis factor interactome (560.7)
McCann K, Charette J, Vincent N, Baserga S. Discovering the pre‐60S ribosome biogenesis factor interactome (560.7). The FASEB Journal 2014, 28 DOI: 10.1096/fasebj.28.1_supplement.560.7.Peer-Reviewed Original ResearchRibosome assemblyGlycerol gradient sedimentation analysisTwo-hybrid analysisHigh-throughput yeastHigh-confidence interactionsPotential regulatory mechanismsGradient sedimentation analysisRNA helicasesBiogenesis factorsSmaller subcomplexesMDa complexNucleolar proteinsRibosomal subunitRegulatory mechanismsDifferent proteinsSubcomplexHelicasesGlycerol gradientsIndependent validation experimentsInteractomeSedimentation analysisYeastCurrent knowledgeProteinAssembly
2011
Small Ribonucleoproteins in Ribosome Biogenesis
Bleichert F, Baserga S. Small Ribonucleoproteins in Ribosome Biogenesis. Protein Reviews 2011, 135-156. DOI: 10.1007/978-1-4614-0514-6_7.ChaptersSmall nucleolar RNPsSite of functionNon-enzymatic functionsRibosome biogenesisFunctional ribosomesEukaryotic cellsSmall ribonucleoproteinSmall RNPsGuide RNANucleotide modificationsRNA componentRNA substratesSubstrate RNAEndonucleolytic cleavageMacromolecular assembliesRNA foldingCurrent understandingRNARNPNucleoliEnzymeLarge macromoleculesAssemblyChaperonesBiogenesis