2016
The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development
Sondalle SB, Baserga SJ, Yelick PC. The Contributions of the Ribosome Biogenesis Protein Utp5/WDR43 to Craniofacial Development. Journal Of Dental Research 2016, 95: 1214-1220. PMID: 27221611, PMCID: PMC5076753, DOI: 10.1177/0022034516651077.Peer-Reviewed Original ResearchConceptsRibosomal proteinsCraniofacial developmentRibosome biogenesis proteinsRibosomal protein functionHuman craniofacial developmentTissue-specific defectsDiverse cell typesHuman ribosomopathiesBiogenesis proteinsTranslational machineryProtein functionDistinct functionsTranslational mechanismsTissue differentiationCell typesWDR43Global defectsProteinExciting researchSurprising similaritiesUnderstanding of rolesRibosomopathiesBiogenesisMachineryMutations
2014
Making Ribosomes: Pre-rRNA Transcription and Processing
McCann K, Baserga S. Making Ribosomes: Pre-rRNA Transcription and Processing. 2014, 217-232. DOI: 10.1007/978-3-319-05687-6_9.Peer-Reviewed Original Research
2013
The novel zebrafish mutant fantome/wdr43 as a human craniofacial ribosomopathy model
LaBonty M, Zhao C, McCann K, Andreeva V, Baserga S, Yelick P. The novel zebrafish mutant fantome/wdr43 as a human craniofacial ribosomopathy model. The FASEB Journal 2013, 27: 319.1-319.1. DOI: 10.1096/fasebj.27.1_supplement.319.1.Peer-Reviewed Original ResearchMRNA injectionNeural crest cell populationsCrest cell populationsSubcellular localization studiesQRT-PCRStable cell linesWISH analysesNorthern blot analysisPremature stop codonMutant exhibitsRRNA biogenesisRibosomal proteinsFan mutantsPositional cloningHuman neurocristopathiesAntisense morpholino oligomersCraniofacial developmentSubcellular localizationFan alleleNucleolar phosphoproteinWDR43Stop codonP53 signalingLocalization studiesBlot analysis
2011
The initial U3 snoRNA:pre‐rRNA base‐pairing interaction required for pre‐18S rRNA folding revealed by in vivo chemical probing
Baserga S, Dutca L, Gallagher J. The initial U3 snoRNA:pre‐rRNA base‐pairing interaction required for pre‐18S rRNA folding revealed by in vivo chemical probing. The FASEB Journal 2011, 25: 704.1-704.1. DOI: 10.1096/fasebj.25.1_supplement.704.1.Peer-Reviewed Original ResearchU3 snoRNABase-pairing interactionsSmall subunitRibosomal subunitU3 small nucleolar RNAPre-rRNA foldingVivo chemical probingSmall nucleolar RNAsSmall ribosomal subunitRRNA foldingEukaryotic nucleolusRibosomal proteinsU3 snoRNPCytoplasmic ribosomesNucleolar RNAsGenetic manipulationS. cerevisiaeChemical probingSnoRNAsVivo chemicalRRNAEssential processSubunitsFoldingNucleotide reactivity
2000
A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis
Dunbar D, Dragon F, Lee S, Baserga S. A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 13027-13032. PMID: 11087857, PMCID: PMC27172, DOI: 10.1073/pnas.97.24.13027.Peer-Reviewed Original ResearchConceptsNucleolar proteinsLarge ribosomal subunit biogenesisLarge ribosomal subunit RNARibosomal subunit biogenesisRNA-binding domainRibosomal subunit RNAPulse-chase labeling experimentsProcessing of precursorsRibosomal protein L7Subunit biogenesisExonucleolytic trimmingEssential genesRibosomal proteinsSpecificity factorMature rRNAPrecursor RNASpacer 2L7 proteinSubunit RNAEndonucleolytic cleavageProtein L7Extensive identityRRNAMajor substratePrimer extension
1999
Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing
Lee S, Baserga S. Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing. Molecular And Cellular Biology 1999, 19: 5441-5452. PMID: 10409734, PMCID: PMC84386, DOI: 10.1128/mcb.19.8.5441.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceGenes, ReporterGenetic TechniquesGenetic VectorsMacromolecular SubstancesMolecular Sequence DataPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA PrecursorsRNA, FungalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino AcidConceptsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinProtein componentsU3 snoRNPNucleolar ribonucleoproteinGenetic depletionTwo-hybrid methodologyNovel protein componentsPre-rRNA processingUnique protein componentRibosomal proteinsSnoRNA levelsRRNA processingU3 snoRNARRNA precursorIMP proteinU3 RNAS4 familiesPrecise functionSevere defectsCleavage eventsMpp10pProteinSnoRNPsRNA