2000
A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis
Dunbar D, Dragon F, Lee S, Baserga S. A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 13027-13032. PMID: 11087857, PMCID: PMC27172, DOI: 10.1073/pnas.97.24.13027.Peer-Reviewed Original ResearchConceptsNucleolar proteinsLarge ribosomal subunit biogenesisLarge ribosomal subunit RNARibosomal subunit biogenesisRNA-binding domainRibosomal subunit RNAPulse-chase labeling experimentsProcessing of precursorsRibosomal protein L7Subunit biogenesisExonucleolytic trimmingEssential genesRibosomal proteinsSpecificity factorMature rRNAPrecursor RNASpacer 2L7 proteinSubunit RNAEndonucleolytic cleavageProtein L7Extensive identityRRNAMajor substratePrimer extension
1999
Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing
Lee S, Baserga S. Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing. Molecular And Cellular Biology 1999, 19: 5441-5452. PMID: 10409734, PMCID: PMC84386, DOI: 10.1128/mcb.19.8.5441.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceGenes, ReporterGenetic TechniquesGenetic VectorsMacromolecular SubstancesMolecular Sequence DataPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA PrecursorsRNA, FungalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino AcidConceptsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinProtein componentsU3 snoRNPNucleolar ribonucleoproteinGenetic depletionTwo-hybrid methodologyNovel protein componentsPre-rRNA processingUnique protein componentRibosomal proteinsSnoRNA levelsRRNA processingU3 snoRNARRNA precursorIMP proteinU3 RNAS4 familiesPrecise functionSevere defectsCleavage eventsMpp10pProteinSnoRNPsRNA
1997
Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10
Lee S, Baserga S. Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 13536-13541. PMID: 9391061, PMCID: PMC28341, DOI: 10.1073/pnas.94.25.13536.Peer-Reviewed Original Research