A New Strategy for Identifying Mechanisms of Drug-drug Interaction Using Transcriptome Analysis: Compound Kushen Injection as a Proof of Principle
Shen H, Qu Z, Harata-Lee Y, Cui J, Aung TN, Wang W, Kortschak RD, Adelson DL. A New Strategy for Identifying Mechanisms of Drug-drug Interaction Using Transcriptome Analysis: Compound Kushen Injection as a Proof of Principle. Scientific Reports 2019, 9: 15889. PMID: 31685921, PMCID: PMC6828681, DOI: 10.1038/s41598-019-52375-3.Peer-Reviewed Original ResearchConceptsTranscriptome analysisCompound Kushen InjectionCo-expression analysisPotential targetPotential molecular mechanismsTranscriptome dataImportant genesMDA-MB-231 cellsProof of principlePhenotype resultsMolecular mechanismsCytotoxic effectsMetabolic processesMajor regulatorA431 cellsAntagonistic cytotoxic effectsKushen InjectionDNA synthesisCancer cellsInhibition of MyD88GenesComplex herbal mixturesMyD88 geneChemotherapy drugsCancer chemotherapy drugsUnderstanding the Mechanistic Contribution of Herbal Extracts in Compound Kushen Injection With Transcriptome Analysis
Shen H, Qu Z, Harata-Lee Y, Aung TN, Cui J, Wang W, Kortschak RD, Adelson DL. Understanding the Mechanistic Contribution of Herbal Extracts in Compound Kushen Injection With Transcriptome Analysis. Frontiers In Oncology 2019, 9: 632. PMID: 31380274, PMCID: PMC6660286, DOI: 10.3389/fonc.2019.00632.Peer-Reviewed Original ResearchTranscriptome analysisCompound Kushen InjectionPlant secondary metabolitesSingle herb extractsTranscriptome dataHerbal compatibilityDNA replicationKushen InjectionImmune-related pathwaysMDA-MB-231 cellsGene expressionCell cycleIL-1β protein expressionSecondary metabolitesIL-1β gene expressionTraditional Chinese medicine formulationChinese medicine formulationMechanistic contributionsProtein expressionELISA assaysCytotoxic effectsHerbal medicinePathwayHerbal extractsMedicine formulation