Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle
Aung TN, Nourmohammadi S, Qu Z, Harata-Lee Y, Cui J, Shen HY, Yool AJ, Pukala T, Du H, Kortschak RD, Wei W, Adelson DL. Fractional Deletion of Compound Kushen Injection Indicates Cytokine Signaling Pathways are Critical for its Perturbation of the Cell Cycle. Scientific Reports 2019, 9: 14200. PMID: 31578346, PMCID: PMC6775143, DOI: 10.1038/s41598-019-50271-4.Peer-Reviewed Original ResearchConceptsCompound Kushen InjectionExperimental biology approachesEffect of CKIPlant secondary metabolitesCytokine Signaling PathwaysCKI activityBiology approachReconstitution approachGene expressionCell cyclePhenotype dataSignaling pathwaysSecondary metabolitesBreast cancer cell linesCancer cell linesKushen InjectionSingle major compoundCell linesApoptosisLiquid chromatography fractionationCell viabilityHigh-performance liquid chromatography fractionationMajor compoundsComplex fashionCandidate mechanismUnderstanding the Mechanistic Contribution of Herbal Extracts in Compound Kushen Injection With Transcriptome Analysis
Shen H, Qu Z, Harata-Lee Y, Aung TN, Cui J, Wang W, Kortschak RD, Adelson DL. Understanding the Mechanistic Contribution of Herbal Extracts in Compound Kushen Injection With Transcriptome Analysis. Frontiers In Oncology 2019, 9: 632. PMID: 31380274, PMCID: PMC6660286, DOI: 10.3389/fonc.2019.00632.Peer-Reviewed Original ResearchTranscriptome analysisCompound Kushen InjectionPlant secondary metabolitesSingle herb extractsTranscriptome dataHerbal compatibilityDNA replicationKushen InjectionImmune-related pathwaysMDA-MB-231 cellsGene expressionCell cycleIL-1β protein expressionSecondary metabolitesIL-1β gene expressionTraditional Chinese medicine formulationChinese medicine formulationMechanistic contributionsProtein expressionELISA assaysCytotoxic effectsHerbal medicinePathwayHerbal extractsMedicine formulation