2024
Regulation and signaling of the LIM domain kinases
Casanova‐Sepúlveda G, Boggon T. Regulation and signaling of the LIM domain kinases. BioEssays 2024, e2400184. PMID: 39361252, DOI: 10.1002/bies.202400184.Peer-Reviewed Original ResearchLIM domain kinaseDownstream of Rho GTPasesCofilin/actin depolymerizing factorActin cytoskeleton regulationIntra-molecular mechanismFilament severingDepolymerizing factorRho GTPasesActin depolymerizationCytoskeleton regulationRegulation mechanismKinaseLIMProteinRegulationGTPaseLIMK2LIMK1ActinEnzymeHuman healthSignalDepolymerizationCascadeMechanism
2022
Tandem engagement of phosphotyrosines by the dual SH2 domains of p120RasGAP
Stiegler A, Vish K, Boggon T. Tandem engagement of phosphotyrosines by the dual SH2 domains of p120RasGAP. Structure 2022, 30: 1603-1614.e5. PMID: 36417908, PMCID: PMC9722645, DOI: 10.1016/j.str.2022.10.009.Peer-Reviewed Original ResearchConceptsGTPase-activating proteinsSH2 domainSH2-SH3Src homology 2 domainDual SH2 domainsPhosphotyrosine residuesSH3 domainRho GTPasesPhosphotyrosine recognitionTarget proteinsSynergistic bindingPhosphotyrosineP120RasGAPConformational flexibilityProteinSelectivity mechanismAffinity measurementsDomainGTPasesClose proximityCassetteCrystal structureResiduesCompact arrangementBindingRho family GTPase signaling through type II p21-activated kinases
Chetty A, Ha B, Boggon T. Rho family GTPase signaling through type II p21-activated kinases. Cellular And Molecular Life Sciences 2022, 79: 598. PMID: 36401658, PMCID: PMC10105373, DOI: 10.1007/s00018-022-04618-2.Peer-Reviewed Original ResearchConceptsRho family small GTPasesP21-activated kinaseRho GTPasesSmall GTPasesPAK family membersRho family GTPaseSignal transduction pathwaysMechanism of regulationPAK familySignal transductionTransduction pathwaysGTPasesMolecular basisDownstream effectorsDomain recognitionPAKsCross talkKinasePAK groupDistinct structuresRegulationPAKFamily membersGTPaseTransduction