2023
Organ function is preserved despite reorganization of niche architecture in the hair follicle
Wei H, Du S, Parksong J, Pasolli H, Matte-Martone C, Regot S, Gonzalez L, Xin T, Greco V. Organ function is preserved despite reorganization of niche architecture in the hair follicle. Cell Stem Cell 2023, 30: 962-972.e6. PMID: 37419106, PMCID: PMC10362479, DOI: 10.1016/j.stem.2023.06.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationEpitheliumHairHair FollicleMiceTransforming Growth Factor betaConceptsNiche architectureDermal papilla fibroblastsDifferentiated lineagesHair follicle growthStereotypic architectureMultipotent progenitorsEpithelial progenitorsFunctional importanceNicheStem cellsFibroblast nicheProgenitorsPowerful modelIntravital imagingDermal papillaFibroblastsHair folliclesFollicle growthOrgan functionLineagesDifferentiationCrosstalkHairProliferationCells
2019
Lymph vessels find a hairy niche
Kam CY, Greco V. Lymph vessels find a hairy niche. The EMBO Journal 2019, 38: embj2019103219. PMID: 31531872, PMCID: PMC6769378, DOI: 10.15252/embj.2019103219.Peer-Reviewed Original ResearchHair follicle regeneration suppresses Ras-driven oncogenic growth
Pineda CM, Gonzalez DG, Matte-Martone C, Boucher J, Lathrop E, Gallini S, Fons NR, Xin T, Tai K, Marsh E, Nguyen DX, Suozzi KC, Beronja S, Greco V. Hair follicle regeneration suppresses Ras-driven oncogenic growth. Journal Of Cell Biology 2019, 218: 3212-3222. PMID: 31488583, PMCID: PMC6781447, DOI: 10.1083/jcb.201907178.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinogenesisHair FollicleMiceMice, TransgenicNeoplasmsRas ProteinsRegenerationConceptsHair folliclesHras mutationsOncogenic growthHair follicle stem cellsSkin hair folliclesTumor developmentFollicle stem cellsHair follicle regenerationSkin epitheliumSecondary mutationsBenign outgrowthFolliclesStem cellsTissueCertain tissuesFollicle regenerationCellsContinuous tissueWild-type neighborsDistinct mechanismsDifferent outcomesMutationsEnhanced capacityInjury
2018
Flexible fate determination ensures robust differentiation in the hair follicle
Xin T, Gonzalez D, Rompolas P, Greco V. Flexible fate determination ensures robust differentiation in the hair follicle. Nature Cell Biology 2018, 20: 1361-1369. PMID: 30420661, PMCID: PMC6314017, DOI: 10.1038/s41556-018-0232-y.Peer-Reviewed Original ResearchConceptsSingle-cell levelStem cellsStem cell differentiationGerm stem cellsTissue architectureMultiple cell typesFate determinationDetermination mechanismTissue homeostasisSame stem cellsCommon progenitorDifferentiation outcomesDifferentiation stimuliDifferentiation lineageCell differentiationCell typesNormal differentiationWnt activationHair folliclesUnanticipated flexibilityDifferentiationRobust differentiationProgenitorsCellsUninjured condition
2015
Intravital imaging of hair follicle regeneration in the mouse
Pineda CM, Park S, Mesa KR, Wolfel M, Gonzalez DG, Haberman AM, Rompolas P, Greco V. Intravital imaging of hair follicle regeneration in the mouse. Nature Protocols 2015, 10: 1116-1130. PMID: 26110716, PMCID: PMC4632978, DOI: 10.1038/nprot.2015.070.Peer-Reviewed Original ResearchThe Dynamic Duo: Niche/Stem Cell Interdependency
Mesa KR, Rompolas P, Greco V. The Dynamic Duo: Niche/Stem Cell Interdependency. Stem Cell Reports 2015, 4: 961-966. PMID: 26028534, PMCID: PMC4471832, DOI: 10.1016/j.stemcr.2015.05.001.Peer-Reviewed Original ResearchConceptsNormal tissue homeostasisStem cell behaviorStem cell interactionsTissue homeostasisCellular turnoverPathological settingsCell behaviorStem cellsMost tissuesFundamental processesDynamic duoCell interactionsModel systemNew cellsCell productionTissue regenerationCellsTissueLive miceOrganismsHomeostasisTremendous advancesImaging approachNovel imaging approachBasic understandingNiche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool
Mesa KR, Rompolas P, Zito G, Myung P, Sun TY, Brown S, Gonzalez DG, Blagoev KB, Haberman AM, Greco V. Niche-induced cell death and epithelial phagocytosis regulate hair follicle stem cell pool. Nature 2015, 522: 94-97. PMID: 25849774, PMCID: PMC4457634, DOI: 10.1038/nature14306.Peer-Reviewed Original Research
2014
Valentina Greco: Got hair?
Greco V, Sedwick C. Valentina Greco: Got hair? Journal Of Cell Biology 2014, 207: 436-437. PMID: 25422370, PMCID: PMC4242828, DOI: 10.1083/jcb.2074pi.Peer-Reviewed Original ResearchSpontaneous tumour regression in keratoacanthomas is driven by Wnt/retinoic acid signalling cross-talk
Zito G, Saotome I, Liu Z, Ferro EG, Sun TY, Nguyen DX, Bilguvar K, Ko CJ, Greco V. Spontaneous tumour regression in keratoacanthomas is driven by Wnt/retinoic acid signalling cross-talk. Nature Communications 2014, 5: 3543. PMID: 24667544, PMCID: PMC3974217, DOI: 10.1038/ncomms4543.Peer-Reviewed Original ResearchConceptsTumor regressionRetinoic acidSquamous cell carcinomaSpontaneous tumor regressionMouse model systemCell carcinomaMalignant tumorsTherapeutic strategiesCutaneous keratoacanthomasKeratoacanthomaPhysiological mechanismsTumor growthHuman keratoacanthomaTumorsHair follicle regenerationCancer biologyFollicle regenerationRegressionDifferentiation programWntCarcinomaPathwayβ-Catenin Activation Regulates Tissue Growth Non–Cell Autonomously in the Hair Stem Cell Niche
Deschene ER, Myung P, Rompolas P, Zito G, Sun TY, Taketo MM, Saotome I, Greco V. β-Catenin Activation Regulates Tissue Growth Non–Cell Autonomously in the Hair Stem Cell Niche. Science 2014, 343: 1353-1356. PMID: 24653033, PMCID: PMC4096864, DOI: 10.1126/science.1248373.Peer-Reviewed Original ResearchConceptsWild-type cellsWnt/β-catenin signalingΒ-catenin signalingΒ-catenin activationMouse hair follicle stem cellsΒ-cateninStem cell nicheHair follicle stem cellsFollicle stem cellsNiche signalsMutant cellsCell divisionCell nicheCoordinated regenerationHair growthWnt ligandsCellular displacementCell behaviorStem cellsHair regenerationTissue growthSignalingCellsTissue regenerationActivation
2013
Stem cell dynamics in the hair follicle niche
Rompolas P, Greco V. Stem cell dynamics in the hair follicle niche. Seminars In Cell And Developmental Biology 2013, 25: 34-42. PMID: 24361866, PMCID: PMC3988239, DOI: 10.1016/j.semcdb.2013.12.005.Peer-Reviewed Original ResearchConceptsHair follicle nicheStem cellsStem cell behaviorMouse genetic modelsNon-cellular componentsNiche microenvironmentMolecular signalsCell behaviorNicheMammalian skinHair folliclesProcess of regenerationCell dynamicsCell populationsGenetic modelsExtracellular materialCurrent understandingCellular compositionTissue regenerationCellsRegenerationNew hairAppendagesMicroenvironmentFolliclesSpatial organization within a niche as a determinant of stem-cell fate
Rompolas P, Mesa KR, Greco V. Spatial organization within a niche as a determinant of stem-cell fate. Nature 2013, 502: 513-518. PMID: 24097351, PMCID: PMC3895444, DOI: 10.1038/nature12602.Peer-Reviewed Original ResearchConceptsStem cell fateHair follicle nicheStem cell lineagesStem cellsStem cell nicheHair follicle stem cellsStem cell compartmentFollicle stem cellsFate determinationGenetic lineagesDifferentiated fateAdult tissuesNiche locationsMammalian tissuesNicheHair regenerationLineagesSpatial organizationEpithelial cellsFateCellsHair growthRegenerationLive miceTissue
2012
Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration
Rompolas P, Deschene ER, Zito G, Gonzalez DG, Saotome I, Haberman AM, Greco V. Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration. Nature 2012, 487: 496-499. PMID: 22763436, PMCID: PMC3772651, DOI: 10.1038/nature11218.Peer-Reviewed Original Research
2010
Compartmentalized organization: a common and required feature of stem cell niches?
Greco V, Guo S. Compartmentalized organization: a common and required feature of stem cell niches? Development 2010, 137: 1586-1594. PMID: 20430743, PMCID: PMC2860245, DOI: 10.1242/dev.041103.Peer-Reviewed Original ResearchConceptsStem cell nicheCell nicheHair follicle stem cell nicheFollicle stem cell nicheAdult stem cell nichesStem cellsStem cell fieldOrgan growthNicheHair regenerationSlow cyclingRecent findingsCell fieldNew growthTissue regenerationRecent studiesCellsGrowthLong-term growthRegenerationProgenyCompartmentsKey questions
2009
A Two-Step Mechanism for Stem Cell Activation during Hair Regeneration
Greco V, Chen T, Rendl M, Schober M, Pasolli HA, Stokes N, dela Cruz-Racelis J, Fuchs E. A Two-Step Mechanism for Stem Cell Activation during Hair Regeneration. Cell Stem Cell 2009, 4: 155-169. PMID: 19200804, PMCID: PMC2668200, DOI: 10.1016/j.stem.2008.12.009.Peer-Reviewed Original ResearchConceptsBMP inhibitorsHair germHG cellsStem cellsStem cell activationHair regenerationTransit-amplifying cellsBulge stem cellsDermal papillaCyclic boutsTranscriptional profilingElevated FGFPrecocious activityHair folliclesBulge cellsLate telogenWntCell clustersCell activationCellsTwo-step mechanismSmall cell clustersInitial stepRegenerationInhibitors
2003
Defining the Epithelial Stem Cell Niche in Skin
Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E. Defining the Epithelial Stem Cell Niche in Skin. Science 2003, 303: 359-363. PMID: 14671312, PMCID: PMC2405920, DOI: 10.1126/science.1092436.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell DivisionCell SeparationEpidermal CellsEpidermisEpithelial CellsGene Expression ProfilingGene Expression RegulationGreen Fluorescent ProteinsHair FollicleHistonesKeratinocytesLuminescent ProteinsMiceMice, TransgenicMicroscopy, FluorescenceMultipotent Stem CellsOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTranscription, GeneticConceptsStem cell nicheLabel-retaining cellsCell nicheSkin stem cell nichesCell type-specific fashionType-specific fashionEpithelial stem cell nicheTranscriptional profilesSlow-cycling cellsHigh proliferative capacityMessenger RNANicheSurface receptorsProliferative capacityRegenerative cellsCellsRNAProgenyProtein