2023
Injury prevents Ras mutant cell expansion in mosaic skin
Gallini S, Annusver K, Rahman N, Gonzalez D, Yun S, Matte-Martone C, Xin T, Lathrop E, Suozzi K, Kasper M, Greco V. Injury prevents Ras mutant cell expansion in mosaic skin. Nature 2023, 619: 167-175. PMID: 37344586, PMCID: PMC10322723, DOI: 10.1038/s41586-023-06198-y.Peer-Reviewed Original ResearchConceptsWild-type cellsRas family proteinsCell cycle inhibitor p21Family proteinsOncogenic RasGenetic approachesMosaic tissuesInhibition of EGFRInhibitor p21EGFR ligandsEGFR pathwayCell expansionAberrant growthConstitutive lossDifferential activationParacrine secretionAbsence of injuryCellsCompetitive balanceInjury repairHealthy skinInjurySkinProteinPathwayCell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2023, 222: e202302095. PMID: 37102999, PMCID: PMC10140546, DOI: 10.1083/jcb.202302095.Peer-Reviewed Original Research
2022
Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer
Cockburn K, Annusver K, Gonzalez D, Ganesan S, May D, Mesa K, Kawaguchi K, Kasper M, Greco V. Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer. Nature Cell Biology 2022, 24: 1692-1700. PMID: 36357619, PMCID: PMC9729105, DOI: 10.1038/s41556-022-01021-8.Peer-Reviewed Original ResearchConceptsCell cycle exitCycle exitStem cell layerUndifferentiated stem cellsStem cell compartmentDaughter cellsDifferentiated populationsDifferentiation genesTranscriptional changesCell divisionMultiple progenitorsStemness genesCells transitStem cellsCell compartmentCell layerCell poolDifferentiationRegenerative tissueGenesSkin epidermisGradual differentiationMulti-day processesCells
2020
Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo
Pokrass MJ, Ryan KA, Xin T, Pielstick B, Timp W, Greco V, Regot S. Cell-Cycle-Dependent ERK Signaling Dynamics Direct Fate Specification in the Mammalian Preimplantation Embryo. Developmental Cell 2020, 55: 328-340.e5. PMID: 33091369, PMCID: PMC7658051, DOI: 10.1016/j.devcel.2020.09.013.Peer-Reviewed Original ResearchConceptsFate specificationPreimplantation developmentKinase translocation reporterMammalian preimplantation embryosInner cell massEmbryonic stem cellsSingle cellsDifferent cell typesMulticellular organismsEndogenous taggingDaughter cellsNanog proteinActive ERKNanog levelsERK activityGene expressionPreimplantation embryosCell cycleTrophectoderm cellsERK inhibitionCell typesStem cellsLive embryosCell massEmbryos
2003
Defining the Epithelial Stem Cell Niche in Skin
Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E. Defining the Epithelial Stem Cell Niche in Skin. Science 2003, 303: 359-363. PMID: 14671312, PMCID: PMC2405920, DOI: 10.1126/science.1092436.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell DivisionCell SeparationEpidermal CellsEpidermisEpithelial CellsGene Expression ProfilingGene Expression RegulationGreen Fluorescent ProteinsHair FollicleHistonesKeratinocytesLuminescent ProteinsMiceMice, TransgenicMicroscopy, FluorescenceMultipotent Stem CellsOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTranscription, GeneticConceptsStem cell nicheLabel-retaining cellsCell nicheSkin stem cell nichesCell type-specific fashionType-specific fashionEpithelial stem cell nicheTranscriptional profilesSlow-cycling cellsHigh proliferative capacityMessenger RNANicheSurface receptorsProliferative capacityRegenerative cellsCellsRNAProgenyProtein
1997
DNA rereplication in the presence of mitotic spindle inhibitors in human and mouse fibroblasts lacking either p53 or pRb function.
Di Leonardo A, Khan SH, Linke SP, Greco V, Seidita G, Wahl GM. DNA rereplication in the presence of mitotic spindle inhibitors in human and mouse fibroblasts lacking either p53 or pRb function. Cancer Research 1997, 57: 1013-9. PMID: 9067261.Peer-Reviewed Original ResearchConceptsDNA rereplicationBiochemical signal transduction pathwaysSignal transduction pathwaysCell cycle checkpointsPRb tumor suppressorSpindle assemblyCycle checkpointsTransduction pathwaysRereplicationDownstream eventsMitotic divisionTumor suppressorMouse fibroblastsCell ploidyDNA contentDeficient derivativeHuman fibroblastsSpindle inhibitorsP53Cytogenetic analysisMitotic spindle inhibitorsFibroblastsPolyploidyMitosisSuppressor