2024
Selective utilization of glucose metabolism guides mammalian gastrulation
Cao D, Bergmann J, Zhong L, Hemalatha A, Dingare C, Jensen T, Cox A, Greco V, Steventon B, Sozen B. Selective utilization of glucose metabolism guides mammalian gastrulation. Nature 2024, 634: 919-928. PMID: 39415005, PMCID: PMC11499262, DOI: 10.1038/s41586-024-08044-1.Peer-Reviewed Original ResearchConceptsCellular metabolismMammalian gastrulationHexosamine biosynthetic pathwayTranscription factor networksCellular signaling pathwaysSignaling morphogensGlucose metabolismCellular programmeBiosynthetic pathwayFate acquisitionCell fateHousekeeping natureGenetic mechanismsMesoderm migrationFactor networksERK activationExpression patternsSignaling pathwayDevelopmental processesStem cell modelCell typesSpecialized functionsDevelopmental contextMammalian embryosMouse embryos844 Oncogenic Kras converts ERK signal dynamics to disrupt tissue architecture independently of hyperproliferation
Xin T, Gallini S, Wei H, Gonzalez D, Regot S, Greco V. 844 Oncogenic Kras converts ERK signal dynamics to disrupt tissue architecture independently of hyperproliferation. Journal Of Investigative Dermatology 2024, 144: s147. DOI: 10.1016/j.jid.2024.06.860.Peer-Reviewed Original ResearchOncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation
Xin T, Gallini S, Wei H, Gonzalez D, Matte-Martone C, Machida H, Fujiwara H, Pasolli H, Suozzi K, Gonzalez L, Regot S, Greco V. Oncogenic Kras induces spatiotemporally specific tissue deformation through converting pulsatile into sustained ERK activation. Nature Cell Biology 2024, 26: 859-867. PMID: 38689013, PMCID: PMC11519783, DOI: 10.1038/s41556-024-01413-y.Peer-Reviewed Original ResearchERK signalingStem cellsSquamous cell carcinomaHair folliclesOncogenic KRAS mutationsCell carcinomaKRAS mutationsSustained ERK activationERK signaling dynamicsOncogenic mutationsOncogenic KRASERK activationStem cell behaviorIntravital imagingAbnormal cell divisionModulates specific featuresKrasG12DTissue deformationSpatiotemporally specific mannerSustained ERK signalingMutationsLiving miceFolliclesTissue disruptionSingle-cell levelCorrection: Cell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Correction: Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2024, 223: e20230209503052024c. PMID: 38477880, PMCID: PMC10938063, DOI: 10.1083/jcb.20230209503052024c.Peer-Reviewed Original ResearchThe art of observation: bridging science and art to see the unexpected
Gonzalez L, Wei H, Greco V, Friedlaender L. The art of observation: bridging science and art to see the unexpected. Development 2024, 151 PMID: 38477686, DOI: 10.1242/dev.202786.Peer-Reviewed Original Research
2023
Organ function is preserved despite reorganization of niche architecture in the hair follicle
Wei H, Du S, Parksong J, Pasolli H, Matte-Martone C, Regot S, Gonzalez L, Xin T, Greco V. Organ function is preserved despite reorganization of niche architecture in the hair follicle. Cell Stem Cell 2023, 30: 962-972.e6. PMID: 37419106, PMCID: PMC10362479, DOI: 10.1016/j.stem.2023.06.003.Peer-Reviewed Original ResearchConceptsNiche architectureDermal papilla fibroblastsDifferentiated lineagesHair follicle growthStereotypic architectureMultipotent progenitorsEpithelial progenitorsFunctional importanceNicheStem cellsFibroblast nicheProgenitorsPowerful modelIntravital imagingDermal papillaFibroblastsHair folliclesFollicle growthOrgan functionLineagesDifferentiationCrosstalkHairProliferationCellsInjury prevents Ras mutant cell expansion in mosaic skin
Gallini S, Annusver K, Rahman N, Gonzalez D, Yun S, Matte-Martone C, Xin T, Lathrop E, Suozzi K, Kasper M, Greco V. Injury prevents Ras mutant cell expansion in mosaic skin. Nature 2023, 619: 167-175. PMID: 37344586, PMCID: PMC10322723, DOI: 10.1038/s41586-023-06198-y.Peer-Reviewed Original ResearchConceptsWild-type cellsRas family proteinsCell cycle inhibitor p21Family proteinsOncogenic RasGenetic approachesMosaic tissuesInhibition of EGFRInhibitor p21EGFR ligandsEGFR pathwayCell expansionAberrant growthConstitutive lossDifferential activationParacrine secretionAbsence of injuryCellsCompetitive balanceInjury repairHealthy skinInjurySkinProteinPathwayMechanisms of skin vascular maturation and maintenance captured by longitudinal imaging of live mice
Kam C, Singh I, Gonzalez D, Matte-Martone C, Solá P, Solanas G, Bonjoch J, Marsh E, Hirschi K, Greco V. Mechanisms of skin vascular maturation and maintenance captured by longitudinal imaging of live mice. Cell 2023, 186: 2345-2360.e16. PMID: 37167971, PMCID: PMC10225355, DOI: 10.1016/j.cell.2023.04.017.Peer-Reviewed Original ResearchConceptsAdult endothelial cellsNeonatal endothelial cellsEndothelial cellsVascular maturationAdult homeostasisOrgan growthAdult maintenanceHomeostasisLive miceVessel regressionFundamental mechanismsGlobal ablationLocal ablationMaturationNetwork perfusionVascular plexusBlood vesselsLongitudinal imagingVessel repairMaintenanceVascular architecturePlexusFunctional networksMiceMechanismCell cycle controls long-range calcium signaling in the regenerating epidermis
Moore J, Bhaskar D, Gao F, Matte-Martone C, Du S, Lathrop E, Ganesan S, Shao L, Norris R, Sanz N, Annusver K, Kasper M, Cox A, Hendry C, Rieck B, Krishnaswamy S, Greco V. Cell cycle controls long-range calcium signaling in the regenerating epidermis. Journal Of Cell Biology 2023, 222: e202302095. PMID: 37102999, PMCID: PMC10140546, DOI: 10.1083/jcb.202302095.Peer-Reviewed Original ResearchLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
2022
Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer
Cockburn K, Annusver K, Gonzalez D, Ganesan S, May D, Mesa K, Kawaguchi K, Kasper M, Greco V. Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer. Nature Cell Biology 2022, 24: 1692-1700. PMID: 36357619, PMCID: PMC9729105, DOI: 10.1038/s41556-022-01021-8.Peer-Reviewed Original ResearchConceptsCell cycle exitCycle exitStem cell layerUndifferentiated stem cellsStem cell compartmentDaughter cellsDifferentiated populationsDifferentiation genesTranscriptional changesCell divisionMultiple progenitorsStemness genesCells transitStem cellsCell compartmentCell layerCell poolDifferentiationRegenerative tissueGenesSkin epidermisGradual differentiationMulti-day processesCellsProbing the rules of cell coordination in live tissues by interpretable machine learning based on graph neural networks
Yamamoto T, Cockburn K, Greco V, Kawaguchi K. Probing the rules of cell coordination in live tissues by interpretable machine learning based on graph neural networks. PLOS Computational Biology 2022, 18: e1010477. PMID: 36067226, PMCID: PMC9481156, DOI: 10.1371/journal.pcbi.1010477.Peer-Reviewed Original ResearchLB1016 Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer
Cockburn K, Annusver K, Gonzalez D, Ganesan S, May D, Kawaguchi K, Kasper M, Greco V. LB1016 Gradual differentiation uncoupled from cell cycle exit generates heterogeneity in the epidermal stem cell layer. Journal Of Investigative Dermatology 2022, 142: b32. DOI: 10.1016/j.jid.2022.05.1044.Peer-Reviewed Original ResearchLB892 Distinct endothelial behaviors orchestrate developing versus adult skin vascular responses
Kam C, Singh I, Sola P, Solanas G, Bonjoch J, Matte-Martone C, Gonzalez D, Marsh E, Hirschi K, Greco V. LB892 Distinct endothelial behaviors orchestrate developing versus adult skin vascular responses. Journal Of Investigative Dermatology 2022, 142: b7. DOI: 10.1016/j.jid.2022.05.909.Peer-Reviewed Original ResearchA group approach to growing as a principal investigator
Greco V, Politi K, Eisenbarth S, Colón-Ramos D, Giraldez AJ, Bewersdorf J, Berg DN. A group approach to growing as a principal investigator. Current Biology 2022, 32: r498-r504. PMID: 35671717, DOI: 10.1016/j.cub.2022.04.082.Commentaries, Editorials and LettersFrom start to finish—a molecular link in wound repair
Yun S, Greco V. From start to finish—a molecular link in wound repair. Science 2022, 375: 619-620. PMID: 35143296, DOI: 10.1126/science.abn7411.Peer-Reviewed Original ResearchDouble knockin mice show NF-kB trajectories in immune signaling and aging
6. Rahman S, Aqdas M, Martin EW, Ardori FT, Songkiatisak P, Oh K, Uderhardt S, Yun S, Claybourne QC, McDevitt RA, Greco V#, Germain RN, Tessarollo L, & Sung M. Double knockin mice show NF-kB trajectories in immune signaling and aging. Cell Reports. 2022 Nov 22;41(8). doi.org/10.1016/j.celrep.2022.111682Peer-Reviewed Original Research
2021
Functional cell death, corneoptosis, requires temporally controlled intracellular acidification
Moore JL, Greco V. Functional cell death, corneoptosis, requires temporally controlled intracellular acidification. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2106633118. PMID: 33986147, PMCID: PMC8179175, DOI: 10.1073/pnas.2106633118.Peer-Reviewed Original ResearchSkin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis
Park S, Matte-Martone C, Gonzalez DG, Lathrop EA, May DP, Pineda CM, Moore JL, Boucher JD, Marsh E, Schmitter-Sánchez A, Cockburn K, Markova O, Bellaïche Y, Greco V. Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis. Nature Cell Biology 2021, 23: 476-484. PMID: 33958758, PMCID: PMC8603572, DOI: 10.1038/s41556-021-00670-5.Peer-Reviewed Original ResearchConceptsDendritic epidermal T cellsLangerhans cellsCell typesEpithelial cellsDifferent cell typesMultiple cell typesLive adult miceGTPase Rac1Skin-resident immune cellsNon-random spatial distributionDistribution of LCContinuous turnoverEpidermal cellsEpidermal T cellsIndividual cellsCellular mechanismsEnvironmental insultsHomeostasisBasal epithelial cellsImmune cellsT cellsAdult miceCellsProper architectureEpidermis3D super-resolution deep-tissue imaging in living mice
Velasco MGM, Zhang M, Antonello J, Yuan P, Allgeyer ES, May D, M’Saad O, Kidd P, Barentine AES, Greco V, Grutzendler J, Booth MJ, Bewersdorf J. 3D super-resolution deep-tissue imaging in living mice. Optica 2021, 8: 442-450. PMID: 34239948, PMCID: PMC8243577, DOI: 10.1364/optica.416841.Peer-Reviewed Original ResearchWater-immersion objective lensTwo-photon excitationSuper-resolution imagingEmission depletion (STED) microscopyAdaptive opticsSTED systemObjective lensOptical aberrationsDepletion microscopyBiological tissuesNanoscale structuresLiving mouseOrganic dyesOpticsSTEDExcitationMouse brain tissueLiving cellsThree-dimensional visualizationMicroscopyLightUnique insightsImagingLens