2001
The Sonic Hedgehog Receptor Patched Associates with Caveolin-1 in Cholesterol-rich Microdomains of the Plasma Membrane* 210
Karpen H, Bukowski J, Hughes T, Gratton J, Sessa W, Gailani M. The Sonic Hedgehog Receptor Patched Associates with Caveolin-1 in Cholesterol-rich Microdomains of the Plasma Membrane* 210. Journal Of Biological Chemistry 2001, 276: 19503-19511. PMID: 11278759, DOI: 10.1074/jbc.m010832200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesBlotting, WesternCaveolin 1CaveolinsCell MembraneCholesterolCOS CellsDNA, ComplementaryDrosophila ProteinsElectrophoresis, Polyacrylamide GelGlutathione TransferaseHumansImmunohistochemistryMembrane MicrodomainsMembrane ProteinsMicroscopy, ConfocalModels, BiologicalMolecular Sequence DataMutationPatched ReceptorsPrecipitin TestsProtein BindingProtein Structure, TertiaryProtein TransportReceptors, Cell SurfaceReceptors, G-Protein-CoupledRecombinant Fusion ProteinsSignal TransductionSmoothened ReceptorSubcellular FractionsTime FactorsConceptsCholesterol-rich microdomainsRaft microdomainsCaveolin-1Receptor complexEarly embryonic patterningFractionation studiesHedgehog receptor complexCaveolin-enriched microdomainsBuoyant density fractionsEmbryonic patterningHh proteinsLipid raftsSubcellular localizationPlasma membranePatchedPlasmalemmal cholesterolProtein experimentsImmunoprecipitation studiesSmoothenedMicrodomainsConfocal microscopyImmunocytochemistry dataComplexesMembraneDrosophila
2000
Quantification of eNOS mRNA in the canine cardiac vasculature by competitive PCR
Fulton D, Papapetropoulos A, Zhang X, Catravas J, Hintze T, Sessa W. Quantification of eNOS mRNA in the canine cardiac vasculature by competitive PCR. AJP Heart And Circulatory Physiology 2000, 278: h658-h665. PMID: 10666099, DOI: 10.1152/ajpheart.2000.278.2.h658.Peer-Reviewed Original ResearchConceptsENOS mRNACoronary arteryCoronary microvesselsLeft ventricleENOS cDNAEndothelial nitric oxide synthase (eNOS) mRNANitric oxide synthase mRNAEndothelial cellsCanine aortic endothelial cellsRight coronary arteryENOS mRNA levelsEndothelial cell markersCompetitive PCRAortic endothelial cellsVon Willebrand factorVein endothelial cellsInducible NOSNeuronal NOSCanine vasculatureLarge arteriesCanine Left VentricleCell markersPathophysiological statesArteryCardiac vasculature