2000
Quantification of eNOS mRNA in the canine cardiac vasculature by competitive PCR
Fulton D, Papapetropoulos A, Zhang X, Catravas J, Hintze T, Sessa W. Quantification of eNOS mRNA in the canine cardiac vasculature by competitive PCR. AJP Heart And Circulatory Physiology 2000, 278: h658-h665. PMID: 10666099, DOI: 10.1152/ajpheart.2000.278.2.h658.Peer-Reviewed Original ResearchConceptsENOS mRNACoronary arteryCoronary microvesselsLeft ventricleENOS cDNAEndothelial nitric oxide synthase (eNOS) mRNANitric oxide synthase mRNAEndothelial cellsCanine aortic endothelial cellsRight coronary arteryENOS mRNA levelsEndothelial cell markersCompetitive PCRAortic endothelial cellsVon Willebrand factorVein endothelial cellsInducible NOSNeuronal NOSCanine vasculatureLarge arteriesCanine Left VentricleCell markersPathophysiological statesArteryCardiac vasculature
1996
Characterization of Bovine Endothelial Nitric Oxide Synthase Expressed inE. coli
Martasek P, Liu Q, Liu J, Roman L, Gross S, Sessa W, Masters B. Characterization of Bovine Endothelial Nitric Oxide Synthase Expressed inE. coli. Biochemical And Biophysical Research Communications 1996, 219: 359-365. PMID: 8604992, DOI: 10.1006/bbrc.1996.0238.Peer-Reviewed Original ResearchConceptsE. coliBaculovirus expression systemBovine endothelial nitric oxide synthaseSDS/PAGEHEK-293 cellsChaperonin GroELHeterologous expressionRecombinant enzymeExpression systemExpression vectorActive enzymeFormation assaysEnzymatic activityColiEnzymeTissue sourcesEndothelial constitutive nitric oxide synthaseProteinSingle bandSynthaseNitric oxide synthaseAbsorbance maximumEndothelial nitric oxide synthase
1994
The Nitric Oxide Synthase Family of Proteins
Sessa W. The Nitric Oxide Synthase Family of Proteins. Journal Of Vascular Research 1994, 31: 131-143. PMID: 7511942, DOI: 10.1159/000159039.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid OxidoreductasesAmino Acid SequenceAnimalsArginineCardiovascular SystemCloning, MolecularConsensus SequenceDNAGene Expression Regulation, EnzymologicHumansImmune SystemMolecular Sequence DataNervous SystemNitric OxideNitric Oxide SynthasePhosphorylationSequence AlignmentSequence Homology, Amino AcidConceptsCytochrome P450 reductase domainNitric oxide synthaseP450 reductase domainNitric oxide synthase (NOS) familySynthase familyMammalian enzymeSeparate genesReductase domainMolecular biologyNovel familyIsoforms of NOSImportant familyNitric oxideProteinEndothelial nitric oxide synthaseEnzyme nitric oxide synthaseGuanidino nitrogenImportant bio-regulatory moleculeOxide synthaseFamilyL-arginineCardiovascular systemRecent advancesGenesEnzymology