2012
Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Wu M, De Camilli P. Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding. Methods In Cell Biology 2012, 108: 1-18. PMID: 22325595, DOI: 10.1016/b978-0-12-386487-1.00001-8.Peer-Reviewed Original ResearchConceptsPlasma membranePlasma membrane sheetsNative plasma membraneEndocytic budsCell-free assayMembrane buddingEndocytic eventsMolecular dissectionElucidation of mechanismsCytosolic extractsBiological membranesMembrane sheetsEndocytosisExtracellular materialRobust formationMembraneSubsequent incubationVariety of manipulationsReconstitutionCellsExperimental systemPowerful toolFissionBuddingBuds
2010
Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nature Cell Biology 2010, 12: 902-908. PMID: 20729836, PMCID: PMC3338250, DOI: 10.1038/ncb2094.Peer-Reviewed Original ResearchActinsAcyltransferasesAdenosine TriphosphateAnimalsAntibodiesBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCattleCell LineCell MembraneCell Membrane StructuresCell-Free SystemClathrinCoated Pits, Cell-MembraneCytosolDynaminsEndocytosisFatty Acid-Binding ProteinsFibroblastsGuanosine 5'-O-(3-Thiotriphosphate)Guanosine TriphosphateHumansImaging, Three-DimensionalMiceMicroscopy, Electron, TransmissionMicroscopy, FluorescenceModels, BiologicalPotoroidaeRatsReceptors, TransferrinThiazolidines