2018
Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Yong J, Chen Y, Wu M. Real-Time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System. Methods In Molecular Biology 2018, 1847: 177-187. PMID: 30129017, DOI: 10.1007/978-1-4939-8719-1_13.Peer-Reviewed Original ResearchLight, space, and time in cancer signaling
McPherson PS, Wu M. Light, space, and time in cancer signaling. Molecular Biology Of The Cell 2018, 29: 688-688. PMID: 29535172, PMCID: PMC6003231, DOI: 10.1091/mbc.e17-11-0675.Peer-Reviewed Original ResearchExtracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs
Chen M, Qiu T, Wu J, Yang Y, Wright GD, Wu M, Ge R. Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs. Cell Death & Differentiation 2018, 25: 1905-1920. PMID: 29523874, PMCID: PMC6219483, DOI: 10.1038/s41418-018-0092-9.Peer-Reviewed Original ResearchMeSH KeywordsAngiostatinsApoptosisCell MembraneEndocytosisEndoplasmic Reticulum Chaperone BiPEndosomesFibronectinsFluorescence Recovery After PhotobleachingHeat-Shock ProteinsHuman Umbilical Vein Endothelial CellsHumansMicroscopy, FluorescenceMitochondriaNeovascularization, PhysiologicPhosphoproteinsProtein TransportRecombinant ProteinsRNA InterferenceRNA, Small InterferingSodium-Hydrogen ExchangersSynaptosomal-Associated Protein 25ThrombospondinsConceptsLate endosomesEndosomal proteinExtracellular proteinsT-SNARE proteinsCytosolic scaffold proteinsTotal internal reflection fluorescent microscopyPro-apoptotic functionFluorescent microscopyRegulatory factor 1Recycling endosomesRNAi knockdownCargo degradationScaffold proteinSuper-resolution fluorescent microscopyMitochondrial traffickingCell fractionationPlasma membraneIsthminProximity ligationMitochondriaProtein 25ProteinBiochemical analysisAnti-angiogenic proteinFactor 1
2017
Mitotic Cortical Waves Predict Future Division Sites by Encoding Positional and Size Information
Xiao S, Tong C, Yang Y, Wu M. Mitotic Cortical Waves Predict Future Division Sites by Encoding Positional and Size Information. Developmental Cell 2017, 43: 493-506.e3. PMID: 29161593, DOI: 10.1016/j.devcel.2017.10.023.Peer-Reviewed Original ResearchFFAR2‐FFAR3 receptor heteromerization modulates short‐chain fatty acid sensing
Ang Z, Xiong D, Wu M, Ding JL. FFAR2‐FFAR3 receptor heteromerization modulates short‐chain fatty acid sensing. The FASEB Journal 2017, 32: 289-303. PMID: 28883043, PMCID: PMC5731126, DOI: 10.1096/fj.201700252rr.Peer-Reviewed Original ResearchMeSH KeywordsBeta-Arrestin 2Calcium SignalingColitisCyclic AMPFatty Acids, VolatileFluorescence Resonance Energy TransferHEK293 CellsHumansMacrophagesObesityP38 Mitogen-Activated Protein KinasesPhosphorylationProtein MultimerizationReceptors, Cell SurfaceReceptors, G-Protein-CoupledRecombinant ProteinsConceptsShort-chain fatty acidsBimolecular fluorescence complementationReceptor heteromerizationGut microbiota-derived short-chain fatty acidsMicrobiota-derived short-chain fatty acidsFluorescence complementationFluorescence resonance energy transferHeterologous expressionFree fatty acid receptor 2Mammalian receptorsFatty acidsFatty acid sensingProximity ligationDrug targetsΒ-arrestinHEK293 cellsPrimary human monocytesResonance energy transferNovel pathwayCytosolic CaP38 phosphorylationHeteromersInflammatory monocytesReceptor 2Receptor heteromersProbing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping
Varsano G, Wang Y, Wu M. Probing Mammalian Cell Size Homeostasis by Channel-Assisted Cell Reshaping. Cell Reports 2017, 20: 397-410. PMID: 28700941, DOI: 10.1016/j.celrep.2017.06.057.Peer-Reviewed Original ResearchConceptsCell size homeostasisSize homeostasisSize checkpointCell cycle progressionMammalian cell growthG1/SCell sizeCell reshapingMammalian cellsHomeostasis mechanismsCell growthRat basophilic leukemia cellsCell lengthBasophilic leukemia cellsCheckpointHomeostasisCell size measurementsLeukemia cellsAlternative mechanismCellsRAW 264.7 macrophages
2012
Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding
Wu M, De Camilli P. Chapter 1 Supported Native Plasma Membranes as Platforms for the Reconstitution and Visualization of Endocytic Membrane Budding. Methods In Cell Biology 2012, 108: 1-18. PMID: 22325595, DOI: 10.1016/b978-0-12-386487-1.00001-8.Peer-Reviewed Original ResearchConceptsPlasma membranePlasma membrane sheetsNative plasma membraneEndocytic budsCell-free assayMembrane buddingEndocytic eventsMolecular dissectionElucidation of mechanismsCytosolic extractsBiological membranesMembrane sheetsEndocytosisExtracellular materialRobust formationMembraneSubsequent incubationVariety of manipulationsReconstitutionCellsExperimental systemPowerful toolFissionBuddingBuds
2010
Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system. Nature Cell Biology 2010, 12: 902-908. PMID: 20729836, PMCID: PMC3338250, DOI: 10.1038/ncb2094.Peer-Reviewed Original ResearchActinsAcyltransferasesAdenosine TriphosphateAnimalsAntibodiesBridged Bicyclo Compounds, HeterocyclicCarrier ProteinsCattleCell LineCell MembraneCell Membrane StructuresCell-Free SystemClathrinCoated Pits, Cell-MembraneCytosolDynaminsEndocytosisFatty Acid-Binding ProteinsFibroblastsGuanosine 5'-O-(3-Thiotriphosphate)Guanosine TriphosphateHumansImaging, Three-DimensionalMiceMicroscopy, Electron, TransmissionMicroscopy, FluorescenceModels, BiologicalPotoroidaeRatsReceptors, TransferrinThiazolidines
2005
Lipid segregation and IgE receptor signaling: A decade of progress
Holowka D, Gosse J, Hammond A, Han X, Sengupta P, Smith N, Wagenknecht-Wiesner A, Wu M, Young R, Baird B. Lipid segregation and IgE receptor signaling: A decade of progress. Biochimica Et Biophysica Acta 2005, 1746: 252-259. PMID: 16054713, DOI: 10.1016/j.bbamcr.2005.06.007.Peer-Reviewed Original ResearchConceptsLipid raftsReceptor phosphorylationSrc family kinase LynTransmembrane tyrosine phosphataseLipid segregationPlasma membrane resultsIgE receptor signalingCell surface receptorsActive LynKinase LynTyrosine phosphataseCytoskeletal interactionsSignal transductionRaft environmentMembrane skeletonDisordered regionsReceptor signalingSurface receptorsSegregation of liquidIgE receptorMembrane structureLynPhosphorylationMembrane resultsComplex role