2022
Targeted Suppression of miRNA-33 Using pHLIP Improves Atherosclerosis Regression
Zhang X, Rotllan N, Canfrán-Duque A, Sun J, Toczek J, Moshnikova A, Malik S, Price NL, Araldi E, Zhong W, Sadeghi MM, Andreev OA, Bahal R, Reshetnyak YK, Suárez Y, Fernández-Hernando C. Targeted Suppression of miRNA-33 Using pHLIP Improves Atherosclerosis Regression. Circulation Research 2022, 131: 77-90. PMID: 35534923, PMCID: PMC9640270, DOI: 10.1161/circresaha.121.320296.Peer-Reviewed Original ResearchConceptsMiR-33Gene expressionNature of miRNAsSingle-cell RNA sequencing analysisSingle-cell RNA transcriptomicsAnti-miRNA technologiesRNA sequencing analysisExpression of miRNAsRNA transcriptomicsNew therapeutic opportunitiesEntire pathwayMiRNA therapeuticsAtherosclerotic plaque macrophagesHuman diseasesMiRNAsSequencing analysisSpecific tissuesMetabolic tissuesTargeted suppressionMiR-33 inhibitionProtective miRNAsNumerous diseasesPharmacological inhibitionLipid accumulationTarget effects
2018
MicroRNAs in endothelial cell homeostasis and vascular disease
Fernández-Hernando C, Suárez Y. MicroRNAs in endothelial cell homeostasis and vascular disease. Current Opinion In Hematology 2018, 25: 227-236. PMID: 29547400, PMCID: PMC6175704, DOI: 10.1097/moh.0000000000000424.Peer-Reviewed Original ResearchConceptsVascular diseaseVascular disease preventionPotential therapeutic targetEndothelial cell homeostasisEndothelial cell functionEndothelial dysfunctionEndothelial functionVascular dysfunctionTherapeutic applicationsPotential therapeutic applicationsInvolvement of miRNAsDysregulation of miRNAsEndothelial homeostasisTherapeutic targetDisease preventionDiseaseCell functionRegulatory circuitsCritical modulatorUnanticipated roleTarget genesCell homeostasisDysfunctionMiRNAsHomeostasis
2017
Posttranscriptional regulation of lipid metabolism by non-coding RNAs and RNA binding proteins
Singh AK, Aryal B, Zhang X, Fan Y, Price NL, Suárez Y, Fernández-Hernando C. Posttranscriptional regulation of lipid metabolism by non-coding RNAs and RNA binding proteins. Seminars In Cell And Developmental Biology 2017, 81: 129-140. PMID: 29183708, PMCID: PMC5975105, DOI: 10.1016/j.semcdb.2017.11.026.Peer-Reviewed Original ResearchConceptsLipid metabolismNon-coding RNAImportance of microRNAsNumber of miRNAsRole of lncRNAsLipid-related genesTranscriptional regulationCoding RNAsPosttranscriptional regulationPosttranscriptional levelMiRNA expressionHigh abundanceLncRNAsRNACholesterol homeostasisMiR-33MiR-148aSpecific roleMiRNAsRegulationLipoprotein metabolismRecent findingsMetabolismProteinExpression
2016
MicroRNAs as regulators of endothelial cell functions in cardiometabolic diseases
Araldi E, Suárez Y. MicroRNAs as regulators of endothelial cell functions in cardiometabolic diseases. Biochimica Et Biophysica Acta 2016, 1861: 2094-2103. PMID: 26825686, PMCID: PMC5039046, DOI: 10.1016/j.bbalip.2016.01.013.Peer-Reviewed Original ResearchConceptsSmall non-coding RNAsLipid/energy metabolismCarlos Fernández-HernandoRegulation of ECNon-coding RNAsRole of miRNAsEndothelial cellsYajaira SuárezTissue homeostasisCell deathEndothelial cell functionEnvironmental stimuliEnergy metabolismMicroRNAsCell proliferationImportant functionsPotential therapeutic applicationsCell functionMiRNAsDifferent cardiometabolic diseasesMetabolic imbalanceEC dysfunctionTherapeutic applicationsPresent reviewRelated diseases
2013
MicroRNAs in Metabolic Disease
Fernández-Hernando C, Ramírez CM, Goedeke L, Suárez Y. MicroRNAs in Metabolic Disease. Arteriosclerosis Thrombosis And Vascular Biology 2013, 33: 178-185. PMID: 23325474, PMCID: PMC3740757, DOI: 10.1161/atvbaha.112.300144.BooksConceptsContribution of miRNAsCellular cholesterol exportMiR-33Fatty acid degradationSREBP genesIntronic miRNAMetabolic diseasesFatty acid synthesisHost genesCholesterol exportSpecific miRNAsPhysiological processesLipid homeostasisMiRNAsAcid synthesisAcid degradationCardiometabolic diseasesGenesMicroRNAsGlucose homeostasisCritical roleGlucose metabolismLipoprotein secretionRecent findingsMetabolic control
2011
The Role of MicroRNAs in Cholesterol Efflux and Hepatic Lipid Metabolism
Moore KJ, Rayner KJ, Suárez Y, Fernández-Hernando C. The Role of MicroRNAs in Cholesterol Efflux and Hepatic Lipid Metabolism. Annual Review Of Nutrition 2011, 31: 49-63. PMID: 21548778, PMCID: PMC3612434, DOI: 10.1146/annurev-nutr-081810-160756.Peer-Reviewed Original ResearchConceptsGene expressionSterol response element-binding proteinMiR-33Fatty acid β-oxidationElement-binding proteinFatty acid homeostasisResponse element-binding proteinRole of microRNAsCholesterol effluxIntronic miRNALipid metabolismRNA bindsPosttranscriptional controlUntranslated regionAbundant miRNABiological processesElegant mechanismMiR-122Lipid homeostasisΒ-oxidationAcid homeostasisCell phenotypeMiRNAsHepatic lipid metabolismMicroRNAsMicroRNAs in lipid metabolism
Fernández-Hernando C, Suárez Y, Rayner KJ, Moore KJ. MicroRNAs in lipid metabolism. Current Opinion In Lipidology 2011, 22: 86-92. PMID: 21178770, PMCID: PMC3096067, DOI: 10.1097/mol.0b013e3283428d9d.Peer-Reviewed Original ResearchConceptsFatty acid metabolismPotent post-transcriptional regulatorsLipid metabolismPost-transcriptional regulatorsCholesterol homeostasisMiR-33Multiple physiological processesAcid metabolismFatty acid degradationFatty acid β-oxidationLipid metabolism genesTiny RNAsTranscriptional regulationABC transportersMetabolism genesFatty acid oxidationHDL biogenesisPhysiological processesCell differentiationMiR-27MiRNAsΒ-oxidationMiR-335Cellular levelMiR-370
2010
MicroRNAs Are Necessary for Vascular Smooth Muscle Growth, Differentiation, and Function
Albinsson S, Suarez Y, Skoura A, Offermanns S, Miano JM, Sessa WC. MicroRNAs Are Necessary for Vascular Smooth Muscle Growth, Differentiation, and Function. Arteriosclerosis Thrombosis And Vascular Biology 2010, 30: 1118-1126. PMID: 20378849, PMCID: PMC2880481, DOI: 10.1161/atvbaha.109.200873.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsAortaCell DifferentiationCell ProliferationCells, CulturedDEAD-box RNA HelicasesEmbryo LossEndoribonucleasesGene Expression Regulation, DevelopmentalGenotypeGestational AgeHemorrhageIntegrasesLiver DiseasesMaleMiceMice, KnockoutMicrofilament ProteinsMicroRNAsMuscle DevelopmentMuscle ProteinsMuscle, Smooth, VascularNuclear ProteinsPhenotypeRibonuclease IIIStress FibersTrans-ActivatorsTranscriptional ActivationTransfectionUmbilical ArteriesVasoconstrictionVasodilationConceptsLate embryonic lethalityFundamental cellular processesContractile differentiationContractile protein markersDicer-dependent miRNAsActin stress fibersDeletion of DicerRole of miRNAsDicer resultsOverexpression of microRNAEmbryonic lethalityMiRNA synthesisCellular processesRate-limiting enzymeStress fibersVascular developmentMuscle growthCell typesCellular proliferationMiRNAsVascular smooth muscle growthVascular smooth muscle proliferationMicroRNAsProtein markersDicer
2009
Cutting Edge: TNF-Induced MicroRNAs Regulate TNF-Induced Expression of E-Selectin and Intercellular Adhesion Molecule-1 on Human Endothelial Cells: Feedback Control of Inflammation
Suárez Y, Wang C, Manes TD, Pober JS. Cutting Edge: TNF-Induced MicroRNAs Regulate TNF-Induced Expression of E-Selectin and Intercellular Adhesion Molecule-1 on Human Endothelial Cells: Feedback Control of Inflammation. The Journal Of Immunology 2009, 184: 21-25. PMID: 19949084, PMCID: PMC2797568, DOI: 10.4049/jimmunol.0902369.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedEndothelial CellsE-SelectinFeedback, PhysiologicalGene ExpressionGene Expression RegulationHumansImmunohistochemistryInflammationIntercellular Adhesion Molecule-1MicroRNAsOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionTransfectionTumor Necrosis Factor-alphaConceptsEndothelial cellsGene expressionUntranslated regionHuman endothelial cellsMiRNAsCultured endothelial cellsTarget sequenceMicroRNA pairsNegative feedback controlMiR-31Adhesion moleculesCellsExpressionNeutrophil adhesionE-selectinAdhesion molecule-1AdhesionTransfectionIntercellular adhesion molecule-1MRNAMolecule-1SequenceEndothelial adhesion moleculesSpecific antagonismICAM-1MicroRNAs As Novel Regulators of Angiogenesis
Suárez Y, Sessa WC. MicroRNAs As Novel Regulators of Angiogenesis. Circulation Research 2009, 104: 442-454. PMID: 19246688, PMCID: PMC2760389, DOI: 10.1161/circresaha.108.191270.Peer-Reviewed Original ResearchConceptsInvolvement of miRNAsShort noncoding RNAsPosttranscriptional regulationNoncoding RNAsNovel regulatorKey regulatorNegative regulatorGene expressionAspects of developmentNew blood vesselsRegulatorVascular biologyCurrent experimental evidencePotential therapeutic applicationsMiRNAsMicroRNAsAngiogenic processEndothelial cellsRegulationAbnormal angiogenesisTherapeutic applicationsAngiogenesisRNABiologyHomeostasis
2008
Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis
Suárez Y, Fernández-Hernando C, Yu J, Gerber SA, Harrison KD, Pober JS, Iruela-Arispe ML, Merkenschlager M, Sessa WC. Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 14082-14087. PMID: 18779589, PMCID: PMC2544582, DOI: 10.1073/pnas.0804597105.Peer-Reviewed Original ResearchConceptsEndothelial miRNAsPostnatal angiogenesisMiR-17Posttranscriptional gene regulationLoss of DicerGeneration of miRNAsExpression of miRNAsCluster miR-17Cell-specific inactivationGene regulationAngiogenic responseEndothelial microRNAsInduced expressionMiRNAsAspects of developmentEndothelial cell proliferationVariety of stimuliCell proliferationDicerThrombospondin-1Exogenous VEGFMicroRNAsExpressionEndothelial cellsRegulation
2007
Dicer Dependent MicroRNAs Regulate Gene Expression and Functions in Human Endothelial Cells
Suárez Y, Fernández-Hernando C, Pober JS, Sessa WC. Dicer Dependent MicroRNAs Regulate Gene Expression and Functions in Human Endothelial Cells. Circulation Research 2007, 100: 1164-1173. PMID: 17379831, DOI: 10.1161/01.res.0000265065.26744.17.Peer-Reviewed Original ResearchConceptsGene expressionHuman endothelial cellsEndogenous miRNA levelsImportance of miRNAsMaturation of microRNAsEC gene expressionEndothelial cellsTek/TieKnockdown of DicerDICER-dependent microRNAsRole of DicerMiRNA expression profilesKDR/VEGFR2MiR-222/221Dicer knockdownDependent microRNAsSynthase protein levelsDicerKey regulatorExpression profilesKey enzymePhysiological pathwaysCord formationEndothelial biologyMiRNAs