2008
Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence
Schleicher M, Shepherd BR, Suarez Y, Fernandez-Hernando C, Yu J, Pan Y, Acevedo LM, Shadel GS, Sessa WC. Prohibitin-1 maintains the angiogenic capacity of endothelial cells by regulating mitochondrial function and senescence. Journal Of Cell Biology 2008, 180: 101-112. PMID: 18195103, PMCID: PMC2213620, DOI: 10.1083/jcb.200706072.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCells, CulturedCellular SenescenceCytoskeletonElectron Transport Complex IEndothelial CellsEndothelium, VascularHumansMiceMice, Inbred StrainsMitochondriaMitochondrial MembranesNeovascularization, PhysiologicNeuropeptidesPhosphatidylinositol 3-KinasesProhibitinsProto-Oncogene Proteins c-aktRac GTP-Binding ProteinsRac1 GTP-Binding ProteinReactive Oxygen SpeciesRepressor ProteinsSignal TransductionConceptsProhibitin 1Mitochondrial functionKnockdown of PHB1Inner mitochondrial membraneEndothelial cell motilityEndothelial cellsCytoskeletal rearrangementsMitochondrial membraneVivo angiogenesis assaysCell motilityAngiogenic capacityCellular senescenceReactive oxygen speciesMitochondrial productionFunctional blood vesselsImportant regulatorSenescenceAngiogenesis assayTube formationOxygen speciesVascular homeostasisCellsVascular systemRac1Yeast
2006
Aplidin® induces JNK-dependent apoptosis in human breast cancer cells via alteration of glutathione homeostasis, Rac1 GTPase activation, and MKP-1 phosphatase downregulation
González-Santiago L, Suárez Y, Zarich N, Muñoz-Alonso M, Cuadrado A, Martínez T, Goya L, Iradi A, Sáez-Tormo G, Maier J, Moorthy A, Cato A, Rojas J, Muñoz A. Aplidin® induces JNK-dependent apoptosis in human breast cancer cells via alteration of glutathione homeostasis, Rac1 GTPase activation, and MKP-1 phosphatase downregulation. Cell Death & Differentiation 2006, 13: 1968-1981. PMID: 16543941, DOI: 10.1038/sj.cdd.4401898.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsApoptosisBreast NeoplasmsCalciumCell Cycle ProteinsCopperDepsipeptidesDown-RegulationDual Specificity Phosphatase 1Enzyme ActivationGlutathione DisulfideGlutathione PeroxidaseGlutathione ReductaseHeLa CellsHomeostasisHumansImmediate-Early ProteinsJNK Mitogen-Activated Protein KinasesMembrane PotentialsMiceMitochondrial MembranesOxidative StressPeptides, CyclicPhosphoprotein PhosphatasesProtein Phosphatase 1Protein Tyrosine PhosphatasesRac1 GTP-Binding ProteinReactive Oxygen SpeciesConceptsJun N-terminal kinaseJNK activationRac1 activationGlutathione homeostasisRac1 small GTPaseJNK-dependent apoptosisRac1 GTPase activationMitochondrial membrane potentialN-terminal kinaseMKP-1 phosphataseSmall GTPaseGTPase activationReactive oxygen speciesHuman breast cancer cellsGSSG/GSH ratioCell deathBreast cancer cellsRapid activationExogenous GSHRNA duplexesSustained activationGSH synthesisSpecific Rac1 inhibitorAplidinDownregulation of Rac1