2021
MMAB promotes negative feedback control of cholesterol homeostasis
Goedeke L, Canfrán-Duque A, Rotllan N, Chaube B, Thompson BM, Lee RG, Cline GW, McDonald JG, Shulman GI, Lasunción MA, Suárez Y, Fernández-Hernando C. MMAB promotes negative feedback control of cholesterol homeostasis. Nature Communications 2021, 12: 6448. PMID: 34750386, PMCID: PMC8575900, DOI: 10.1038/s41467-021-26787-7.Peer-Reviewed Original ResearchMeSH KeywordsAlkyl and Aryl TransferasesAnimalsCell Line, TumorCholesterolCholesterol, LDLFeedback, PhysiologicalGene Expression ProfilingHeLa CellsHep G2 CellsHomeostasisHumansHydroxymethylglutaryl CoA ReductasesLiverMice, Inbred C57BLMice, KnockoutPromoter Regions, GeneticReceptors, LDLRNA InterferenceSterol Regulatory Element Binding Protein 2ConceptsCholesterol biosynthesisCholesterol homeostasisMouse hepatic cell lineIntegrative genomic strategyIntricate regulatory networkMaster transcriptional regulatorCellular cholesterol levelsHMGCR activityLDL-cholesterol uptakeCholesterol levelsHuman hepatic cellsSterol contentGenomic strategiesTranscriptional regulatorsRegulatory networksIntracellular cholesterol levelsGene expressionUnexpected roleHepatic cell linesBiosynthesisMMABIntracellular levelsCell linesHomeostasisExpression of SREBP2
2015
Neuregulin-activated ERBB4 induces the SREBP-2 cholesterol biosynthetic pathway and increases low-density lipoprotein uptake
Haskins JW, Zhang S, Means RE, Kelleher JK, Cline GW, Canfrán-Duque A, Suárez Y, Stern DF. Neuregulin-activated ERBB4 induces the SREBP-2 cholesterol biosynthetic pathway and increases low-density lipoprotein uptake. Science Signaling 2015, 8: ra111. PMID: 26535009, PMCID: PMC4666504, DOI: 10.1126/scisignal.aac5124.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorCholesterolFemaleHumansHydroxymethylglutaryl CoA ReductasesLipoproteins, LDLMechanistic Target of Rapamycin Complex 1Multiprotein ComplexesNeuregulin-1Proto-Oncogene Proteins c-aktReceptor, ErbB-4Receptors, LDLSterol Regulatory Element Binding Protein 2TOR Serine-Threonine KinasesConceptsIntracellular domainEGFR family membersLow-density lipoprotein uptakeCholesterol biosynthesisSREBP target genesRapamycin complex 1ErbB4 intracellular domainSite-1 proteaseCholesterol biosynthesis genesSoluble intracellular domainCholesterol biosynthetic pathwayActivation of ErbB4Mammary epithelial cellsInhibition of AktSterol regulatory elementBiosynthesis genesLipoprotein uptakeRegulatory elementsBiosynthetic pathwayTarget genesDevelopmental processesMetabolic remodelingMature formNeuregulin-1Cellular membranesDisruption of the mevalonate pathway induces dNTP depletion and DNA damage
Sánchez C, Martín J, Jin JS, Dávalos A, Zhang W, de la Peña G, Martínez-Botas J, Rodríguez-Acebes S, Suárez Y, Hazen MJ, Gómez-Coronado D, Busto R, Cheng YC, Lasunción MA. Disruption of the mevalonate pathway induces dNTP depletion and DNA damage. Biochimica Et Biophysica Acta 2015, 1851: 1240-1253. PMID: 26055626, DOI: 10.1016/j.bbalip.2015.06.001.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateCarboxy-LyasesCell Cycle CheckpointsCell Line, TumorCell ProliferationCheckpoint Kinase 1DeoxyribonucleosidesDNA DamageDNA ReplicationGene Expression RegulationHalogenationHemiterpenesHistonesHL-60 CellsHumansLymphocytesMevalonic AcidOrganophosphorus CompoundsProtein KinasesRNA, Small InterferingSignal TransductionConceptsMevalonate diphosphate decarboxylaseDiphosphate decarboxylaseCell cycle progressionDNA replicationCycle progressionMevalonate pathwayDNA damageDNA damage responseNon-sterol isoprenoidsCell proliferationInhibition of Chk1Cholesterol biosynthesis pathwayMassive cell deathSubsequent DNA damageΓ-H2AX formationCell cycle arrestReplication stressBiosynthesis pathwayΓ-H2AX fociChk1 activationDamage responseIsopentenyl diphosphateMitosis completionCell divisionDNTP depletion
2012
Mir-33 regulates cell proliferation and cell cycle progression
Cirera-Salinas D, Pauta M, Allen RM, Salerno AG, Ramírez CM, Chamorro-Jorganes A, Wanschel AC, Lasuncion MA, Morales-Ruiz M, Suarez Y, Baldan A, Esplugues E, Fernández-Hernando C. Mir-33 regulates cell proliferation and cell cycle progression. Cell Cycle 2012, 11: 922-933. PMID: 22333591, PMCID: PMC3323796, DOI: 10.4161/cc.11.5.19421.Peer-Reviewed Original ResearchConceptsCell cycle progressionCyclin-dependent kinase 6Cycle progressionCell proliferationCell cycle regulationMiR-33Expression of genesCyclin D1Cell cycle arrestSREBP genesCycle regulationFatty acid metabolismHost genesPosttranscriptional levelGene expressionIntronic sequencesKinase 6Cellular growthCritical regulatorCycle arrestCellular levelLiver regenerationGenesMiR-33 expressionAcid metabolism
2005
Sterol stringency of proliferation and cell cycle progression in human cells
Suárez Y, Fernández C, Ledo B, Martín M, Gómez-Coronado D, Lasunción MA. Sterol stringency of proliferation and cell cycle progression in human cells. Biochimica Et Biophysica Acta 2005, 1734: 203-213. PMID: 15904877, DOI: 10.1016/j.bbalip.2005.02.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell Line, TumorCell ProliferationCholesterolHumansMolecular StructureConceptsCell cycle progressionCycle progressionG2/M phaseMammalian cellsCell proliferationCell growthM phaseRegulator of proliferationSterol response elementCholesterol-free mediumPlasma membraneCell cycleResponse elementIsoprenoid derivativesStructural roleHuman cellsCholesterol biosynthesisSKF 104976Sterol analoguesDifferent sterolsHuman leukemiaCell processesCholesterol analoguesHL-60Concentration-dependent manner
2004
Synergistic upregulation of low-density lipoprotein receptor activity by tamoxifen and lovastatin
Suárez Y, Fernández C, Gómez-Coronado D, Ferruelo AJ, Dávalos A, Martínez-Botas J, Lasunción MA. Synergistic upregulation of low-density lipoprotein receptor activity by tamoxifen and lovastatin. Cardiovascular Research 2004, 64: 346-355. PMID: 15485695, DOI: 10.1016/j.cardiores.2004.06.024.Peer-Reviewed Original ResearchConceptsLDL receptor activityLow-density lipoproteinReceptor activityLow density lipoprotein receptor activityPlasma LDL cholesterol levelsLDL cholesterol levelsLipoprotein receptor activityEstrogen receptor modulatorsBreast cancer therapyReceptor mRNA levelsLDL receptor mRNA levelsLDL receptor expressionCholesterol-lowering activityDose-dependent mannerHigh LDL concentrationsHypolipidemic effectsMOLT-4 cellsCholesterol levelsReceptor expressionReceptor modulatorsEstrogen receptorTamoxifenLDL concentrationLDL uptakeLDL receptorJNK activation is critical for Aplidin™-induced apoptosis
Cuadrado A, González L, Suárez Y, Martínez T, Muñoz A. JNK activation is critical for Aplidin™-induced apoptosis. Oncogene 2004, 23: 4673-4680. PMID: 15122339, DOI: 10.1038/sj.onc.1207636.Peer-Reviewed Original ResearchMeSH KeywordsAntibodies, MonoclonalAntineoplastic AgentsApoptosisBlotting, WesternBreast NeoplasmsCell DivisionCell Line, TumorCell SurvivalDepsipeptidesEnzyme ActivationFemaleFibroblastsHumansMitogen-Activated Protein KinasesNF-kappa BPeptides, CyclicPhosphorylationPrecipitin TestsProto-Oncogene Proteins c-junTranscription Factor AP-1