2023
SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces.
Lee S, Yousafzai M, Mohler K, Yadav V, Amiri S, Szuszkiewicz J, Levchenko A, Rinehart J, Murrell M. SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces. Molecular Biology Of The Cell 2023, 34: ar122. PMID: 37672340, PMCID: PMC10846615, DOI: 10.1091/mbc.e23-03-0103.Peer-Reviewed Original Research
2022
Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells
Kshitiz, Afzal J, Suhail Y, Chang H, Hubbi M, Hamidzadeh A, Goyal R, Liu Y, Sun P, Nicoli S, Dang C, Levchenko A. Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells. Cell Systems 2022, 13: 1048-1064.e7. PMID: 36462504, PMCID: PMC10012408, DOI: 10.1016/j.cels.2022.11.003.Peer-Reviewed Original ResearchConceptsHIF-1α activityActivation of genesChaperone-mediated autophagyCancer cellsCell divisionIndividual cancer cellsRegulated processPatient-derived cancer cellsSubset of cellsMolecular mechanismsFluorescent reportersSingle-cell responsesCancer cell linesCell linesGenesHypoxic tumor cellsHIF-1αHypoxic conditionsCellsBroad suppressionAggressive growthTumor cellsHypoxic cellsOscillatory activityExtracellular lactate
2014
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer
Feng Y, Zhu M, Dangelmajer S, Lee Y, Wijesekera O, Castellanos C, Denduluri A, Chaichana K, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A. Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer. Cell Death & Disease 2014, 5: e1567-e1567. PMID: 25501828, PMCID: PMC4649837, DOI: 10.1038/cddis.2014.521.Peer-Reviewed Original ResearchConceptsHuman adipose-derived mesenchymal stem cellsAdipose-derived mesenchymal stem cellsMesenchymal stem cellsBrain tumor-initiating cellsAnti-inflammatory propertiesStem cellsAmyotrophic lateral sclerosisTumor-associated fibroblastsTumor-initiating cellsBrain cancer cellsMyocardial infarctionHypoxic culture conditionsClinical investigationLateral sclerosisLess cell deathBrain cancerMigratory capabilityCancer cellsLow oxygen tensionOxygen tensionRegenerative potentialMultipotent cellsAnticancer propertiesCell deathHypoxia
2005
Proteomics takes stem cell analyses to another level
Levchenko A. Proteomics takes stem cell analyses to another level. Nature Biotechnology 2005, 23: 828-830. PMID: 16003369, DOI: 10.1038/nbt0705-828.Peer-Reviewed Original ResearchComment on "Oscillations in NF-κB Signaling Control the Dynamics of Gene Expression"
Barken D, Wang C, Kearns J, Cheong R, Hoffmann A, Levchenko A. Comment on "Oscillations in NF-κB Signaling Control the Dynamics of Gene Expression". Science 2005, 308: 52a-52a. PMID: 15802586, PMCID: PMC2821939, DOI: 10.1126/science.1107904.Peer-Reviewed Original ResearchIntercellular transfer of P-glycoprotein mediates acquired multidrug resistance in tumor cells
Levchenko A, Mehta B, Niu X, Kang G, Villafania L, Way D, Polycarpe D, Sadelain M, Larson S. Intercellular transfer of P-glycoprotein mediates acquired multidrug resistance in tumor cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2005, 102: 1933-1938. PMID: 15671173, PMCID: PMC545583, DOI: 10.1073/pnas.0401851102.Peer-Reviewed Original ResearchConceptsFunctional P-gpP-gpTumor cellsMultidrug resistanceP-gp-mediated resistanceP-gp-negative cellsToxic drug concentrationsP-gp-positive cellsIntercellular transferResistant tumor cellsDifferent tumor cell typesTumor cell typesTumor massTumor stromaP-gp transferTumor typesP-glycoproteinStromal elementsDrug resistanceDrug concentrationsTumor samplesBiologic propertiesCancer therapyCell typesCells
2004
The Systems Biology of Glycosylation
Murrell M, Yarema K, Levchenko A. The Systems Biology of Glycosylation. ChemBioChem 2004, 5: 1334-1347. PMID: 15457533, DOI: 10.1002/cbic.200400143.Peer-Reviewed Original ResearchConceptsSystems biologyRegulation of differentiationRegulation of glycosylationEukaryotic cellsBiochemical systems analysisCell regulation processesCell decisionsSignal transductionGlycosylationBiologyRegulation processesRegulationTransductionApoptosisDifferentiationProfound influenceCellsNovel research methodologyRegulatory modules that generate biphasic signal response in biological systems.
Levchenko A, Bruck J, Sternberg P. Regulatory modules that generate biphasic signal response in biological systems. IET Systems Biology 2004, 1: 139-48. PMID: 17052124, DOI: 10.1049/sb:20045014.Peer-Reviewed Original Research
2002
The IκB-NF-κB Signaling Module: Temporal Control and Selective Gene Activation
Hoffmann A, Levchenko A, Scott M, Baltimore D. The IκB-NF-κB Signaling Module: Temporal Control and Selective Gene Activation. Science 2002, 298: 1241-1245. PMID: 12424381, DOI: 10.1126/science.1071914.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCell NucleusChemokine CCL5Chemokine CXCL10Chemokines, CXCComputer SimulationCytoplasmDNA-Binding ProteinsElectrophoretic Mobility Shift AssayFeedback, PhysiologicalGene Expression RegulationHumansI-kappa B ProteinsMiceMice, KnockoutModels, BiologicalNF-kappa BNF-KappaB Inhibitor alphaProto-Oncogene ProteinsSignal TransductionTranscriptional ActivationTumor Cells, CulturedTumor Necrosis Factor-alphaConceptsTranscriptional activator NF-kappaBSelective gene activationKnockout cell linesTemporal controlNF-kappaB inhibitor proteinNF-kappaB responseSignaling modulesCoordinated degradationGene activationMammalian cellsNuclear localizationInhibitor proteinGene expressionIkappaB proteinsSignal-processing characteristicsEpsilon functionNF-kappaB activationCell linesNF-kappaBModeling the Cell's Guidance System
Iglesias P, Levchenko A. Modeling the Cell's Guidance System. Science Signaling 2002, 2002: re12. PMID: 12209053, DOI: 10.1126/stke.2002.148.re12.Peer-Reviewed Original Research
2000
Evaluation of 11C-colchicine for PET imaging of multiple drug resistance.
Levchenko A, Mehta B, Lee J, Humm J, Augensen F, Squire O, Kothari P, Finn R, Leonard E, Larson S. Evaluation of 11C-colchicine for PET imaging of multiple drug resistance. Journal Of Nuclear Medicine 2000, 41: 493-501. PMID: 10716325.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsATP Binding Cassette Transporter, Subfamily B, Member 1Carbon RadioisotopesColchicineDrug Resistance, MultipleFlow CytometryHumansMiceMice, Inbred BALB CModels, TheoreticalNeuroblastomaRatsRats, NudeTissue DistributionTomography, Emission-ComputedTransplantation, HeterologousTumor Cells, CulturedConceptsResistant tumorsPET imagingMultiple drug resistance phenotypeDrug-resistant variantsQuality of lifeCell linesMultiple drug resistanceResistant cell linesC cell lineP-gp actionDrug resistance phenotypeEarly diagnosisNude ratsFDG scansTumorsP-glycoproteinDrug resistanceBiodistribution experimentsCytotoxic agentsChemotherapeutic interventionResistant strainsCancer cellsIntracellular accumulationVivo experimentsScans