2024
Spatial–temporal order–disorder transition in angiogenic NOTCH signaling controls cell fate specification
Kang T, Bocci F, Nie Q, Onuchic J, Levchenko A. Spatial–temporal order–disorder transition in angiogenic NOTCH signaling controls cell fate specification. ELife 2024, 12: rp89262. PMID: 38376371, PMCID: PMC10942579, DOI: 10.7554/elife.89262.Peer-Reviewed Original ResearchConceptsCell fate specificationFate specificationNotch signalingMorphogenic processesCell-cell communicationComplex morphogenic processesCell fateDynamics of spatial patternsDepletion of fibronectinTip cellsSprout extensionAngiogenic morphogenesisHypoxic micro-environmentCell plasticityCellsComputational analysisPre-existing onesCell patternMicro-environmentSpatial patternsLocal enrichmentMorphogenesisEndothelial cellsAngiogenesis modelFibronectinSpatial–temporal order–disorder transition in angiogenic NOTCH signaling controls cell fate specification
Kang T, Bocci F, Nie Q, Onuchic J, Levchenko A. Spatial–temporal order–disorder transition in angiogenic NOTCH signaling controls cell fate specification. ELife 2024, 12 DOI: 10.7554/elife.89262.3.Peer-Reviewed Original ResearchCell fate specificationStalk cellsCell type-specificTip cellsNotch signalingFate specificationStalk cell differentiationMorphogenic processesDensity of fibronectinType-specificCell typesCell-cell communicationComplex morphogenic processesResponse to elevated levelsCell fateCell identityDynamics of spatial patternsDepletion of fibronectinEnvironmental cuesSignaling moleculesBlood vessel sproutingSprout extensionAngiogenic morphogenesisSignaling pathwayHypoxic micro-environment
2023
Single-Cell Measurements and Modeling and Computation of Decision-Making Errors in a Molecular Signaling System with Two Output Molecules
Emadi A, Lipniacki T, Levchenko A, Abdi A. Single-Cell Measurements and Modeling and Computation of Decision-Making Errors in a Molecular Signaling System with Two Output Molecules. Biology 2023, 12: 1461. PMID: 38132287, PMCID: PMC10740708, DOI: 10.3390/biology12121461.Peer-Reviewed Original ResearchSPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces.
Lee S, Yousafzai M, Mohler K, Yadav V, Amiri S, Szuszkiewicz J, Levchenko A, Rinehart J, Murrell M. SPAK-dependent cotransporter activity mediates capillary adhesion and pressure during glioblastoma migration in confined spaces. Molecular Biology Of The Cell 2023, 34: ar122. PMID: 37672340, PMCID: PMC10846615, DOI: 10.1091/mbc.e23-03-0103.Peer-Reviewed Original Research
2022
Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells
Kshitiz, Afzal J, Suhail Y, Chang H, Hubbi M, Hamidzadeh A, Goyal R, Liu Y, Sun P, Nicoli S, Dang C, Levchenko A. Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells. Cell Systems 2022, 13: 1048-1064.e7. PMID: 36462504, PMCID: PMC10012408, DOI: 10.1016/j.cels.2022.11.003.Peer-Reviewed Original ResearchConceptsHIF-1α activityActivation of genesChaperone-mediated autophagyCancer cellsCell divisionIndividual cancer cellsRegulated processPatient-derived cancer cellsSubset of cellsMolecular mechanismsFluorescent reportersSingle-cell responsesCancer cell linesCell linesGenesHypoxic tumor cellsHIF-1αHypoxic conditionsCellsBroad suppressionAggressive growthTumor cellsHypoxic cellsOscillatory activityExtracellular lactate
2020
CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA
Schiapparelli P, Meade P, Miranda-Herrera P, Bechtle A, Issacs F, Levchenko A, Rinehart J, Quinones-Hinojosa A. CSIG-08. TARGETING ION TRANSPORT-REGULATORY KINASES AS A NOVEL TREATMENT FOR GLIOBLASTOMA. Neuro-Oncology 2020, 22: ii29-ii29. PMCID: PMC7650317, DOI: 10.1093/neuonc/noaa215.120.Peer-Reviewed Original ResearchOxidative stress-responsive kinase 1Small molecule inhibitorsGBM cellsMolecule inhibitorsAggressive primary brain tumorCell migrationComplete surgical resectionMaximal safe resectionPrimary brain tumorsAdjacent brain parenchymaCell proliferationOrthotopic murine modelPatient-derived GBM cell linesDose-dependent reductionImportant therapeutic componentGBM cell linesCell cycle analysisGBM cell migrationSurgical resectionSafe resectionBrain parenchymaCell infiltrationCell cycle arrestMurine modelRadiation therapySignaling Diversity Enabled by Rap1 and cAMP/PKA‐Regulated Plasma Membrane ERK with Distinct Temporal Dynamics
Keyes J, Ganesan A, Molinar-Inglis O, Hamidzadeh A, Ling M, Trejo J, Levchenko A, Zhang J. Signaling Diversity Enabled by Rap1 and cAMP/PKA‐Regulated Plasma Membrane ERK with Distinct Temporal Dynamics. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.00680.Peer-Reviewed Original ResearchERK activityTemporal regulationPrecise temporal regulationMembrane protrusion dynamicsSequence-specific motifsSpecific subcellular locationsControl cell morphologyDifferent subcellular compartmentsMultiple cellular processesERK enzymatic activityCAMP/PKAERK biosensorEGF inducesKinase cascadeCellular processesExtracellular signalsSubcellular compartmentsSubcellular locationProtrusion dynamicsSubcellular regionsPlasma membraneSpecific motifsEnzymatic activityCell morphologyRap1
2016
CSIG-05. PUTTING THE BRAKES ON BRACHYURY: IDENTIFYING AND TARGETING A CONSERVED TRANSCRIPTIONAL NETWORK THAT DRIVES PRIMARY AND METASTATIC TUMOR INITIATION AND PROPAGATION
Shah S, David J, Tippens N, Mohyeldin A, Martinez J, Ganaha S, Levchenko A, Palena C, Quinones-Hinojosa A. CSIG-05. PUTTING THE BRAKES ON BRACHYURY: IDENTIFYING AND TARGETING A CONSERVED TRANSCRIPTIONAL NETWORK THAT DRIVES PRIMARY AND METASTATIC TUMOR INITIATION AND PROPAGATION. Neuro-Oncology 2016, 18: vi41-vi41. DOI: 10.1093/neuonc/now212.166.Peer-Reviewed Original ResearchTranscriptional networksTranscriptional programsGenetic inhibitorsT-box transcription factorCell transcriptional programDirect transcriptional activationCell cycle progressionNew molecular insightsMalignant neoplasmsNegative feedback loopEarly embryogenesisTranscriptional activationHippo pathwayOrgan developmentTranscription factorsMaster regulatorProtein stabilityBioinformatics analysisGenetic stabilityPrevalent lung cancerCycle progressionMetastatic brain tumorsMolecular underpinningsMolecular insightsBrachyury217 YAP Is Ready to Rac and Rho
Shah S, Tippens N, Park J, Mohyeldin A, Vela G, Martinez-Gutierrez J, Margolis S, Schmidt S, Levchenko A, Quinones-Hinojosa A. 217 YAP Is Ready to Rac and Rho. Neurosurgery 2016, 63: 185. DOI: 10.1227/01.neu.0000489786.22041.2d.Peer-Reviewed Original ResearchFundamental Limits to the Precision of Multicellular Sensing
Fancher S, Levchenko A, Nemenman I, Mugler A. Fundamental Limits to the Precision of Multicellular Sensing. Biophysical Journal 2016, 110: 350a. DOI: 10.1016/j.bpj.2015.11.1884.Peer-Reviewed Original ResearchMechanics of Microenvironment as Instructive Cues Guiding Stem Cell Behavior
Kshitiz, Afzal J, Chang H, Goyal R, Levchenko A. Mechanics of Microenvironment as Instructive Cues Guiding Stem Cell Behavior. Current Stem Cell Reports 2016, 2: 62-72. DOI: 10.1007/s40778-016-0033-9.Peer-Reviewed Original ResearchStem cell biologyStem cell behaviorMechanical cuesCell biologyCell behaviorFundamental stem cell biologyNovel bioengineering toolsIntracellular signal transductionStem cell phenotypeExtracellular stimuliSignal transductionAdult tissuesInstructive cuesBioengineering toolsFuture therapeutic applicationsExtracellular matrixStem cellsComplex milieuCell phenotypeCulture conditionsRegenerative medicineMechanotransductionBiologyTopographical architectureTherapeutic applications
2015
Erratum: Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer
Feng Y, Zhu M, Dangelmajer S, Lee Y, Wijesekera O, Castellanos C, Denduluri A, Chaichana K, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A. Erratum: Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer. Cell Death & Disease 2015, 6: e1797-e1797. PMID: 26111059, PMCID: PMC4669846, DOI: 10.1038/cddis.2015.176.Peer-Reviewed Original Research
2014
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer
Feng Y, Zhu M, Dangelmajer S, Lee Y, Wijesekera O, Castellanos C, Denduluri A, Chaichana K, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A. Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer. Cell Death & Disease 2014, 5: e1567-e1567. PMID: 25501828, PMCID: PMC4649837, DOI: 10.1038/cddis.2014.521.Peer-Reviewed Original ResearchConceptsHuman adipose-derived mesenchymal stem cellsAdipose-derived mesenchymal stem cellsMesenchymal stem cellsBrain tumor-initiating cellsAnti-inflammatory propertiesStem cellsAmyotrophic lateral sclerosisTumor-associated fibroblastsTumor-initiating cellsBrain cancer cellsMyocardial infarctionHypoxic culture conditionsClinical investigationLateral sclerosisLess cell deathBrain cancerMigratory capabilityCancer cellsLow oxygen tensionOxygen tensionRegenerative potentialMultipotent cellsAnticancer propertiesCell deathHypoxiaTM-01IDENTIFICATION OF GLUCOSE-6-PHOSPHATASE ALPHA AS A KEY METABOLIC REGULATOR FOR GLIOBLASTOMA CELL INVASION
Abbadi S, Rodarte J, Abutaleb A, Lavell E, Smith C, Ruff W, Schiller J, Olivi A, Levchenko A, Guerrero-Cazares H, Quinones-Hinojosa A. TM-01IDENTIFICATION OF GLUCOSE-6-PHOSPHATASE ALPHA AS A KEY METABOLIC REGULATOR FOR GLIOBLASTOMA CELL INVASION. Neuro-Oncology 2014, 16: v213-v213. PMCID: PMC4218619, DOI: 10.1093/neuonc/nou278.1.Peer-Reviewed Original ResearchBrain tumor initiating cellsGlycolytic inhibitionGlioblastoma cell invasionSuch reprogrammingKnockdown cellsMetabolic reprogrammingTumor initiating cellsKey enzymeGlycogen synthaseCell survivalAggressive primary brain cancerCell invasionComplete rescueFunctional consequencesPotential therapeutic targetPrimary brain cancerGBM cellsWild-type miceG6PCReprogrammingInitiating cellsGlycogen accumulationGlycogen phosphorylaseMalignant progressionKnockdownCS-31A NOVEL YAP-DRIVEN MIGRATION AND INVASION SIGNALING PATHWAY PREDICTS POOR OUTCOME IN GLIOBLASTOMA
Shah S, Tippens N, Park J, Mohyeldin A, Vela G, Levchenko A, Quinones-Hinojosa A. CS-31A NOVEL YAP-DRIVEN MIGRATION AND INVASION SIGNALING PATHWAY PREDICTS POOR OUTCOME IN GLIOBLASTOMA. Neuro-Oncology 2014, 16: v57-v58. PMCID: PMC4217989, DOI: 10.1093/neuonc/nou242.31.Peer-Reviewed Original ResearchCell migration pathwaysMechanism of YAPYAP-dependent mechanismsAbility of cellsDependent signaling mechanismRole of YAPFunction of YAPSmall Rho GTPaseExtracellular cuesTranscriptional regulatorsRho GTPaseSingle-cell mannerMolecular networksIntracellular signalingSignaling mechanismCell mannerCell polarizationCell migrationIndividual cellsCritical modulatorAggressive cancerYAPInvasive behaviorPoor patient prognosisYAP expression
2013
Hypoxia-inducible factor–dependent breast cancer–mesenchymal stem cell bidirectional signaling promotes metastasis
Chaturvedi P, Gilkes D, Wong C, Kshitiz K, Luo W, Zhang H, Wei H, Takano N, Schito L, Levchenko A, Semenza G. Hypoxia-inducible factor–dependent breast cancer–mesenchymal stem cell bidirectional signaling promotes metastasis. Journal Of Clinical Investigation 2013, 123: 1402-1402. PMCID: PMC3674862, DOI: 10.1172/jci69244.Peer-Reviewed Original ResearchIlluminating Passive Permeability Barrier of Primary Cilia using Novel Diffusion Trap Technique
Inoue T, Lin Y, Lin B, Phua S, Jiao J, Levchenko A, Niewiadomski P, Rohatgi R, Nakamura H, Inoue T. Illuminating Passive Permeability Barrier of Primary Cilia using Novel Diffusion Trap Technique. Biophysical Journal 2013, 104: 31a-32a. DOI: 10.1016/j.bpj.2012.11.212.Peer-Reviewed Original Research
2012
Novel Physical Attributes Of Airway Smooth Muscle In The Pathogenesis Of Asthma
An S, Levchenko A, Ahn K, Kogut P, Koziel-White C, Wang R, Lee D, Camoretti-Mercado B, Panettieri R, Solway J. Novel Physical Attributes Of Airway Smooth Muscle In The Pathogenesis Of Asthma. 2012, a2694-a2694. DOI: 10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a2694.Peer-Reviewed Original ResearchERK-Mediated Decoding of Calcium Oscillations in Pancreatic Beta Cells
Ganesan A, Levchenko A, Zhang J. ERK-Mediated Decoding of Calcium Oscillations in Pancreatic Beta Cells. Biophysical Journal 2012, 102: 507a. DOI: 10.1016/j.bpj.2011.11.2777.Peer-Reviewed Original Research
2011
Science Signaling Podcast: 4 October 2011
Levchenko A, VanHook A. Science Signaling Podcast: 4 October 2011. Science Signaling 2011, 4 DOI: 10.1126/scisignal.2002543.Peer-Reviewed Original Research