2024
Highly Reactive Group I Introns Ubiquitous in Pathogenic Fungi
Liu T, Pyle A. Highly Reactive Group I Introns Ubiquitous in Pathogenic Fungi. Journal Of Molecular Biology 2024, 436: 168513. PMID: 38447889, DOI: 10.1016/j.jmb.2024.168513.Peer-Reviewed Original ResearchGroup I intronsAntifungal drug targetsRNA metabolismPathogenic fungiPhylogeny of fungiSelf-splicing intronsDrug targetsSystemic fungal infectionsGenetic hotspotsRiboregulatory elementsMitochondrial intronsMitochondrial genesBioinformatics pipelineC. aurisCandida aurisRelevant fungiRNA elementsAspergillus fumigatusC. albicansHousekeeping genesCandida albicansNoncoding transcriptomeCryptococcus neoformansFungal infectionsFungi
2020
Sequencing and Structure Probing of Long RNAs Using MarathonRT: A Next-Generation Reverse Transcriptase
Guo LT, Adams RL, Wan H, Huston NC, Potapova O, Olson S, Gallardo CM, Graveley BR, Torbett BE, Pyle AM. Sequencing and Structure Probing of Long RNAs Using MarathonRT: A Next-Generation Reverse Transcriptase. Journal Of Molecular Biology 2020, 432: 3338-3352. PMID: 32259542, PMCID: PMC7556701, DOI: 10.1016/j.jmb.2020.03.022.Peer-Reviewed Original ResearchConceptsLong RNA moleculesLong RNAsRNA moleculesRNA base modificationsGroup II intronsMutational profilingTranscriptome compositionRNA metabolismRNA researchBase modificationsPrimer extensionTool enzymeDiverse aspectsMixed populationEnzymeReverse transcriptionRNAStructural complexityReverse transcriptaseProfilingTranscriptase enzymeIntronsTranscription