2021
In vitro fluorescence assay to measure GDP/GTP exchange of guanine nucleotide exchange factors of Rho family GTPases
Blaise AM, Corcoran EE, Wattenberg ES, Zhang YL, Cottrell JR, Koleske AJ. In vitro fluorescence assay to measure GDP/GTP exchange of guanine nucleotide exchange factors of Rho family GTPases. Biology Methods And Protocols 2021, 7: bpab024. PMID: 35087952, PMCID: PMC8789339, DOI: 10.1093/biomethods/bpab024.Peer-Reviewed Original ResearchNucleotide exchange factorsGEF domainExchange factorGuanine nucleotide exchange factorsGDP/GTP exchangeRho family GEFsActivation of GTPasesFluorescence-based analysisRare variantsGTP loadingGTP exchangeRho familyHigh-throughput screeningWhole-exome sequencingCellular activitiesGenetic variantsGTPasesGEFExome sequencingCritical roleUser-friendly protocolComplete understandingHuman developmentVariantsGEFs
2003
Phospholipids Can Switch the GTPase Substrate Preference of a GTPase-activating Protein*
Ligeti E, Dagher MC, Hernandez SE, Koleske AJ, Settleman J. Phospholipids Can Switch the GTPase Substrate Preference of a GTPase-activating Protein*. Journal Of Biological Chemistry 2003, 279: 5055-5058. PMID: 14699145, DOI: 10.1074/jbc.c300547200.Peer-Reviewed Original ResearchConceptsGTPase-activating proteinsSmall GTPasesSubstrate preferencePotent GTPase-activating proteinMajor cellular inhibitorActivity of GTPasesNovel regulatory mechanismLikely physiological roleRacGAP activityIntrinsic GTPRhoGAP activityP190 RhoGAPCellular inhibitorRac GTPasesGTPasesRegulatory mechanismsPhysiological roleProteinPhospholipidsFatty acidsRhoGAPGTPP190ActivityRho