2018
False-Positive Light Chain Clonal Restriction by Flow Cytometry in Patients Treated With Alemtuzumab
Chen PP, Tormey CA, Eisenbarth SC, Torres R, Richardson SS, Rinder HM, Smith BR, Siddon AJ. False-Positive Light Chain Clonal Restriction by Flow Cytometry in Patients Treated With Alemtuzumab. American Journal Of Clinical Pathology 2018, 151: 154-163. PMID: 30307483, DOI: 10.1093/ajcp/aqy129.Peer-Reviewed Original ResearchConceptsHealthy donor bloodAlemtuzumab treatmentDonor bloodBone marrowFlow cytometryLight chain restrictionT-cell prolymphocytic leukemiaB-cell neoplasmsLight chain clonalityFlow cytometry analysisAutoimmune diseasesHematologic malignanciesImmunophenotypic analysisChain restrictionAlemtuzumabT cellsB cellsProlymphocytic leukemiaImmunoglobulin G1PatientsMonoclonal antibodiesCytometry analysisSimilar findingsBloodClonal restriction
2012
Utility of peripheral blood flow cytometry in differentiating low grade versus high grade myelodysplastic syndromes (MDS) and in the evaluation of cytopenias.
Rashidi HH, Xu X, Wang HY, Shafi NQ, Rameshkumar K, Messer K, Smith BR, Rose MG. Utility of peripheral blood flow cytometry in differentiating low grade versus high grade myelodysplastic syndromes (MDS) and in the evaluation of cytopenias. International Journal Of Clinical And Experimental Pathology 2012, 5: 224-30. PMID: 22558477, PMCID: PMC3341683.Peer-Reviewed Original ResearchConceptsHigh-grade myelodysplastic syndromeChronic myelomonocytic leukemiaPeripheral blood flow cytometryEvaluation of cytopeniasBlood flow cytometryMyelodysplastic syndromeFlow cytometryDifferential diagnosisHigh-risk myelodysplastic syndromeLow-grade myelodysplastic syndromesRisk myelodysplastic syndromesCMML patientsMyelomonocytic leukemiaDiagnostic utilityCytopeniasPatientsCytometrySyndromeLow gradeDiagnosisLeukemia
2008
DNA ploidy analysis as an adjunct for the detection of relapse in B-lineage acute lymphoblastic leukemia
Kenney B, Zieske A, Rinder H, Smith B. DNA ploidy analysis as an adjunct for the detection of relapse in B-lineage acute lymphoblastic leukemia. Leukemia & Lymphoma 2008, 49: 42-48. PMID: 18203010, DOI: 10.1080/10428190701760052.Peer-Reviewed Original ResearchConceptsAcute lymphoblastic leukemiaDetection of relapseDNA ploidy analysisLymphoblastic leukemiaPloidy analysisB-lineageB-lineage acute lymphoblastic leukemiaCytometric DNA ploidy analysisDiagnosis of relapseNormal precursor B cellsPrecursor B cellsOriginal immunophenotypeRelapsed diseaseUnique immunophenotypeLeukemic immunophenotypesImmunophenotypic detectionRelapseB cellsPatientsImmunophenotypeFlow immunophenotypingFlow cytometryAneuploid populationsDiagnosisLeukemia
2007
Flow cytometric analysis of myelomonocytic cells by a pattern recognition approach is sensitive and specific in diagnosing myelodysplastic syndrome and related marrow diseases: Emphasis on a global evaluation and recognition of diagnostic pitfalls
Stachurski D, Smith BR, Pozdnyakova O, Andersen M, Xiao Z, Raza A, Woda BA, Wang A. Flow cytometric analysis of myelomonocytic cells by a pattern recognition approach is sensitive and specific in diagnosing myelodysplastic syndrome and related marrow diseases: Emphasis on a global evaluation and recognition of diagnostic pitfalls. Leukemia Research 2007, 32: 215-224. PMID: 17675229, DOI: 10.1016/j.leukres.2007.06.012.Peer-Reviewed Original ResearchConceptsMyelodysplastic syndromeFlow cytometryDiagnostic pitfallsMyelomonocytic cellsMDS/MPDClonal cytogenetic abnormalitiesGlobal evaluationImmunophenotypic aberranciesRetrospective reviewMorphological dysplasiaMarrow diseaseMyeloproliferative diseaseDiagnostic utilityCytogenetic abnormalitiesDisease casesMyeloid diseasesCytometric analysisOverall sensitivityAbnormalitiesDiseaseOverall abnormalitySyndromeIntermediate category
1998
Morphologic, Immunophenotypic, and Molecular Evaluation of Bone Marrow Involvement in Non-Hodgkin's Lymphoma
Crotty P, Smith B, Tallini G. Morphologic, Immunophenotypic, and Molecular Evaluation of Bone Marrow Involvement in Non-Hodgkin's Lymphoma. Applied Immunohistochemistry & Molecular Morphology 1998, 7: 90-95. PMID: 9785007, DOI: 10.1097/00019606-199804000-00005.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDBiomarkers, TumorBone MarrowBone Marrow ExaminationClone CellsFlow CytometryGene Rearrangement, B-LymphocyteGene Rearrangement, T-LymphocyteHumansImmunophenotypingLymphoma, B-CellLymphoma, Non-HodgkinLymphoma, T-CellNeoplasm ProteinsNeoplastic Stem CellsPolymerase Chain ReactionSensitivity and SpecificityConceptsPrimer polymerase chain reactionMarrow involvementT-cell neoplasiaFlow cytometryB-cell neoplasiaObjective evidenceT-cell receptor gene rearrangementsBone marrow involvementConsensus primer polymerase chain reactionNon-Hodgkin lymphomaChronic lymphocytic leukemiaB-cell lymphomaT-cell neoplasmsB-cell neoplasmsReceptor gene rearrangementsDetection of clonalityDetectable diseaseHodgkin's lymphomaPolymerase chain reactionLymphocytic leukemiaApparent diseaseLymphomaPatientsImportant adjunctNeoplasia
1997
Epstein‐Barr virus suspension cell assay using in situ hybridization and flow cytometry
Crouch J, Leitenberg D, Smith B, Howe J. Epstein‐Barr virus suspension cell assay using in situ hybridization and flow cytometry. Cytometry 1997, 29: 50-57. PMID: 9298811, DOI: 10.1002/(sici)1097-0320(19970901)29:1<50::aid-cyto5>3.0.co;2-h.Peer-Reviewed Original Research
1993
Determination of the percentage of thiazole orange (TO)‐positive, “reticulated” platelets using autologous erythrocyte TO fluorescence as an internal standard
Bonan J, Rinder H, Smith B. Determination of the percentage of thiazole orange (TO)‐positive, “reticulated” platelets using autologous erythrocyte TO fluorescence as an internal standard. Cytometry 1993, 14: 690-694. PMID: 8404376, DOI: 10.1002/cyto.990140615.Peer-Reviewed Original Research
1991
Modulation of Platelet Surface Adhesion Receptors during Cardiopulmonary Bypass
Rinder C, Mathew J, Rinder H, Bonan J, Ault K, Smith B. Modulation of Platelet Surface Adhesion Receptors during Cardiopulmonary Bypass. Anesthesiology 1991, 75: 563-570. PMID: 1718190, DOI: 10.1097/00000542-199110000-00004.Peer-Reviewed Original ResearchConceptsIIb/IIIaCardiopulmonary bypassGlycoprotein IIb/IIIaPlatelet activationQualitative platelet defectsGlycoprotein IVGlycoprotein IbAlpha-granule releaseGranule membrane protein-140Surface glycoprotein IbHLA APlatelet functionBaseline valuesVon Willebrand factor receptorBypassThrombospondin receptorGlycoprotein Ib expressionFlow cytometryIb expressionPlatelet defectsSelective decreasePlatelet receptorsFactor receptorReceptorsProtein 140
1988
Cytoplasmic transfer between endothelium and lymphocytes: quantitation by flow cytometry.
Guinan EC, Smith BR, Davies PF, Pober JS. Cytoplasmic transfer between endothelium and lymphocytes: quantitation by flow cytometry. American Journal Of Pathology 1988, 132: 406-9. PMID: 3414774, PMCID: PMC1880752.Peer-Reviewed Original Research
1987
CTL adhesion and antigen recognition are discrete steps in the human CTL-target cell interaction.
Mentzer SJ, Smith BR, Barbosa JA, Crimmins MA, Herrmann SH, Burakoff SJ. CTL adhesion and antigen recognition are discrete steps in the human CTL-target cell interaction. The Journal Of Immunology 1987, 138: 1325-30. PMID: 3543126, DOI: 10.4049/jimmunol.138.5.1325.Peer-Reviewed Original ResearchConceptsHLA-A2CTL clonesHLA-B7Target cellsAntigen recognitionHuman CTL clonesTarget cell lysisCalcium-sensitive dye indo-1Cytolytic T lymphocytesCTL-target interactionDye indo-1CTL-target cell interactionAntigen-specific receptorsT cell receptorCell surface moleculesCTL activationT lymphocytesHuman CTLIndo-1LFA-1Free calciumCell receptorFlow cytometrySurface moleculesCell interactions
1983
In Waldenstrom's macroglobulinemia the quantity of detectable circulating monoclonal B lymphocytes correlates with clinical course.
Smith BR, Robert NJ, Ault KA. In Waldenstrom's macroglobulinemia the quantity of detectable circulating monoclonal B lymphocytes correlates with clinical course. Blood 1983, 61: 911-4. PMID: 6403083, DOI: 10.1182/blood.v61.5.911.bloodjournal615911.Peer-Reviewed Original ResearchConceptsMonoclonal B lymphocytesWaldenstrom's macroglobulinemiaB lymphocytesSerum IgM levelsAbnormal cellsMonoclonal surfaceBlood involvementAsymptomatic presentationClinical responseClinical courseIgM levelsMultiple myelomaClinical activitySerial determinationsSerial studiesPatientsSurface immunoglobulinMacroglobulinemiaFlow cytometryLymphocytesMonoclonal cellsOvernight cultureSuch cellsDiseaseCellsIn Waldenstrom's Macroglobulinemia the Quantity of Detectable Circulating Monoclonal B Lymphocytes Correlates With Clinical Course
Smith B, Robert N, Ault K. In Waldenstrom's Macroglobulinemia the Quantity of Detectable Circulating Monoclonal B Lymphocytes Correlates With Clinical Course. Blood 1983, 61: 911-914. DOI: 10.1182/blood.v61.5.911.911.Peer-Reviewed Original ResearchMonoclonal B lymphocytesWaldenstrom's macroglobulinemiaB lymphocytesSerum IgM levelsAbnormal cellsMonoclonal surfaceBlood involvementAsymptomatic presentationClinical responseClinical courseIgM levelsMultiple myelomaClinical activitySerial determinationsSerial studiesPatientsSurface immunoglobulinMacroglobulinemiaFlow cytometryLymphocytesMonoclonal cellsOvernight cultureSuch cellsDiseaseCells