2018
False-Positive Light Chain Clonal Restriction by Flow Cytometry in Patients Treated With Alemtuzumab
Chen PP, Tormey CA, Eisenbarth SC, Torres R, Richardson SS, Rinder HM, Smith BR, Siddon AJ. False-Positive Light Chain Clonal Restriction by Flow Cytometry in Patients Treated With Alemtuzumab. American Journal Of Clinical Pathology 2018, 151: 154-163. PMID: 30307483, DOI: 10.1093/ajcp/aqy129.Peer-Reviewed Original ResearchConceptsHealthy donor bloodAlemtuzumab treatmentDonor bloodBone marrowFlow cytometryLight chain restrictionT-cell prolymphocytic leukemiaB-cell neoplasmsLight chain clonalityFlow cytometry analysisAutoimmune diseasesHematologic malignanciesImmunophenotypic analysisChain restrictionAlemtuzumabT cellsB cellsProlymphocytic leukemiaImmunoglobulin G1PatientsMonoclonal antibodiesCytometry analysisSimilar findingsBloodClonal restriction
2008
DNA ploidy analysis as an adjunct for the detection of relapse in B-lineage acute lymphoblastic leukemia
Kenney B, Zieske A, Rinder H, Smith B. DNA ploidy analysis as an adjunct for the detection of relapse in B-lineage acute lymphoblastic leukemia. Leukemia & Lymphoma 2008, 49: 42-48. PMID: 18203010, DOI: 10.1080/10428190701760052.Peer-Reviewed Original ResearchConceptsAcute lymphoblastic leukemiaDetection of relapseDNA ploidy analysisLymphoblastic leukemiaPloidy analysisB-lineageB-lineage acute lymphoblastic leukemiaCytometric DNA ploidy analysisDiagnosis of relapseNormal precursor B cellsPrecursor B cellsOriginal immunophenotypeRelapsed diseaseUnique immunophenotypeLeukemic immunophenotypesImmunophenotypic detectionRelapseB cellsPatientsImmunophenotypeFlow immunophenotypingFlow cytometryAneuploid populationsDiagnosisLeukemia
2004
Flow Cytometric DNA Ploidy Analysis of Peripheral Blood From Patients With Sézary Syndrome
Wang, Li N, Heald P, Fisk JM, Fadare O, Howe JG, McNiff JM, Smith BR. Flow Cytometric DNA Ploidy Analysis of Peripheral Blood From Patients With Sézary Syndrome. American Journal Of Clinical Pathology 2004, 122: 774-782. PMID: 15491974, DOI: 10.1309/8b849fc6phap8fdd.Peer-Reviewed Original ResearchConceptsDNA ploidy studiesNeoplastic T cellsSezary syndromeMinimal residual diseasePloidy studiesResidual diseaseBlood-based diagnosisT cellsBlood samplesCutaneous T-cell lymphoma casesT-cell lymphoma casesCytometric DNA ploidy analysisBlood tumor burdenPeripheral blood samplesLarge cell transformationLow-grade groupDNA ploidy analysisAneuploid cellsSézary syndromeLymph nodesImmunophenotypic findingsTumor burdenPeripheral bloodLymphoma casesAneuploid groupReal-Time Quantitative Reverse Transcription-PCR for Cyclin D1 mRNA in Blood, Marrow, and Tissue Specimens for Diagnosis of Mantle Cell Lymphoma
Howe JG, Crouch J, Cooper D, Smith BR. Real-Time Quantitative Reverse Transcription-PCR for Cyclin D1 mRNA in Blood, Marrow, and Tissue Specimens for Diagnosis of Mantle Cell Lymphoma. Clinical Chemistry 2004, 50: 80-87. PMID: 14633913, DOI: 10.1373/clinchem.2003.024695.Peer-Reviewed Original ResearchConceptsMantle cell lymphomaReal-time reverse transcription PCRCases of MCLCyclin D1 mRNAQuantitative RT-PCRLymphoproliferative diseaseReverse transcription-PCRCell lymphomaD1 mRNAMicroglobulin mRNADiagnosis of MCLTranscription-PCRCyclin D1 mRNA expressionB cell-specific markersCritical diagnostic markerReal-time quantitative RT-PCRD1 mRNA expressionTime quantitative reverse transcription PCRQuantitative reverse transcription PCROvert diseaseMarrow specimensAmbiguous immunophenotypeCD19 mRNAMalignant cellsTissue specimens
1998
Morphologic, Immunophenotypic, and Molecular Evaluation of Bone Marrow Involvement in Non-Hodgkin's Lymphoma
Crotty P, Smith B, Tallini G. Morphologic, Immunophenotypic, and Molecular Evaluation of Bone Marrow Involvement in Non-Hodgkin's Lymphoma. Applied Immunohistochemistry & Molecular Morphology 1998, 7: 90-95. PMID: 9785007, DOI: 10.1097/00019606-199804000-00005.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDBiomarkers, TumorBone MarrowBone Marrow ExaminationClone CellsFlow CytometryGene Rearrangement, B-LymphocyteGene Rearrangement, T-LymphocyteHumansImmunophenotypingLymphoma, B-CellLymphoma, Non-HodgkinLymphoma, T-CellNeoplasm ProteinsNeoplastic Stem CellsPolymerase Chain ReactionSensitivity and SpecificityConceptsPrimer polymerase chain reactionMarrow involvementT-cell neoplasiaFlow cytometryB-cell neoplasiaObjective evidenceT-cell receptor gene rearrangementsBone marrow involvementConsensus primer polymerase chain reactionNon-Hodgkin lymphomaChronic lymphocytic leukemiaB-cell lymphomaT-cell neoplasmsB-cell neoplasmsReceptor gene rearrangementsDetection of clonalityDetectable diseaseHodgkin's lymphomaPolymerase chain reactionLymphocytic leukemiaApparent diseaseLymphomaPatientsImportant adjunctNeoplasia
1994
B-Cell Precursor Bone Marrow Reconstitution After Bone Marrow Transplantation
Leitenberg D, Rappeport J, Smith B. B-Cell Precursor Bone Marrow Reconstitution After Bone Marrow Transplantation. American Journal Of Clinical Pathology 1994, 102: 231-236. PMID: 8042594, DOI: 10.1093/ajcp/102.2.231.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAntigens, CDB-LymphocytesBlotting, SouthernBone MarrowBone Marrow CellsBone Marrow TransplantationChildChild, PreschoolClone CellsFemaleFlow CytometryFluorescent Antibody TechniqueGene Rearrangement, B-Lymphocyte, Heavy ChainHematopoietic Stem CellsHumansImmunophenotypingInfantKineticsMaleMiddle AgedNeprilysinConceptsBone marrow transplantationMarrow transplantationB cell subsetsBone marrow reconstitutionB cell precursorsSurface antigenic phenotypeImmature B cellsImmunoglobulin gene rearrangementsHumoral immunodeficiencyMonoclonal gammapathyMarrow reconstitutionAntigenic phenotypeB cellsTransplantationGene rearrangementsMarked increaseLeukemia lymphoblastsCellsPatientsGammapathyImmunodeficiencyMarrowCD34ReconstitutionMonths
1993
Qualitative versus Quantitative Immunophenotypinga
SMITH B. Qualitative versus Quantitative Immunophenotypinga. Annals Of The New York Academy Of Sciences 1993, 677: 152-154. PMID: 8494204, DOI: 10.1111/j.1749-6632.1993.tb38773.x.Peer-Reviewed Original ResearchIntegrating Flow Cytometry in the Hematology Laboratory
SMITH B. Integrating Flow Cytometry in the Hematology Laboratory. Annals Of The New York Academy Of Sciences 1993, 677: 326-333. PMID: 8494218, DOI: 10.1111/j.1749-6632.1993.tb38787.x.Peer-Reviewed Original ResearchPhenotypic and Functional Analysis of Expanded Natural Killer Cell Subpopulations in Hodgkin’s Disease Patients Treated with Lymphoid Irradiation
Macklis R, Mauch P, Thompson L, Burakoff S, Smith B. Phenotypic and Functional Analysis of Expanded Natural Killer Cell Subpopulations in Hodgkin’s Disease Patients Treated with Lymphoid Irradiation. Oncology 1993, 50: 323-328. PMID: 8497384, DOI: 10.1159/000227203.Peer-Reviewed Original ResearchMeSH KeywordsCytotoxicity Tests, ImmunologicFlow CytometryHodgkin DiseaseHumansImmunophenotypingKiller Cells, NaturalLymphatic IrradiationConceptsNK cell subsetsNK cell compartmentHodgkin's disease patientsDisease patientsLymphoid irradiationNK cellsCell subsetsNatural killer cell subpopulationsSpecific NK cell subsetsLong-term immunosuppressionNK cell assaysCell compartmentSignificant changesCell subpopulationsPatientsCytometric cell sortingLytic activityCell assayImmunophenotypeCell sortingCytotoxicity spectrumImmunosuppressionCellsSubsetCompartments