2024
Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging
Schueder F, Rivera-Molina F, Su M, Marin Z, Kidd P, Rothman J, Toomre D, Bewersdorf J. Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging. Cell 2024, 187: 1769-1784.e18. PMID: 38552613, DOI: 10.1016/j.cell.2024.02.033.Peer-Reviewed Original ResearchConceptsInter-organelle contactsSuper-resolutionMultiplexed super-resolution microscopyIntricate spatial relationshipsGolgi stacksMammalian cellsCellular functionsSuper-resolution microscopyPrimary ciliaSuper-resolution fluorescence microscopyCellular complexityTransient adaptationFluorescence microscopyDNA-PAINTFluorogenic labelingMolecular targetsSpatial relationshipsImagesThroughput
2019
New software for automated cilia detection in cells (ACDC)
Lauring MC, Zhu T, Luo W, Wu W, Yu F, Toomre D. New software for automated cilia detection in cells (ACDC). Cilia 2019, 8: 1. PMID: 31388414, PMCID: PMC6670212, DOI: 10.1186/s13630-019-0061-z.Peer-Reviewed Original ResearchManual analysisNew softwareData setsHighest F1 scoreTraditional manual analysisSoftware applicationsF1 scoreDetection accuracyUser biasRepresentative imagesInput dataSoftwareIntensity standard deviationEntire data setMain stepsParameter combinationsFalse positivesExperimental data setsKey metricsImagesSpeed enhancementAccuracyFalse negative rateMicroscopic imagesImage magnification