2024
Cell surface RNAs control neutrophil recruitment
Zhang N, Tang W, Torres L, Wang X, Ajaj Y, Zhu L, Luan Y, Zhou H, Wang Y, Zhang D, Kurbatov V, Khan S, Kumar P, Hidalgo A, Wu D, Lu J. Cell surface RNAs control neutrophil recruitment. Cell 2024, 187: 846-860.e17. PMID: 38262409, PMCID: PMC10922858, DOI: 10.1016/j.cell.2023.12.033.Peer-Reviewed Original ResearchConceptsCell surfaceMammalian homologOuter cell surfaceRNA transportGlycan modificationsMammalian cellsSID-1Cellular functionsRecruitment to inflammatory sitesGlycoRNARNAMurine neutrophilsFunctional significanceNeutrophil recruitmentNeutrophil recruitment to inflammatory sitesBiological importanceCellsNeutrophil adhesionReduced neutrophil adhesionHomologyGlycansGenesInflammatory sitesRecruitmentEndothelial cells
2021
Dickkopf-2 regulates the stem cell marker LGR5 in colorectal cancer via HNF4α1
Shin JH, Jeong J, Choi J, Lim J, Dinesh RK, Braverman J, Hong JY, Maher SE, Vesely M, Kim W, Koo JH, Tang W, Wu D, Blackburn HN, Xicola RM, Llor X, Yilmaz O, Choi JM, Bothwell ALM. Dickkopf-2 regulates the stem cell marker LGR5 in colorectal cancer via HNF4α1. IScience 2021, 24: 102411. PMID: 33997693, PMCID: PMC8099562, DOI: 10.1016/j.isci.2021.102411.Peer-Reviewed Original ResearchColorectal cancerDickkopf-2Colitis-associated cancerColorectal cancer stemnessStem cell marker Lgr5Colonic epithelial cellsAggressive progressionCancer stemnessLGR5 expressionColonic organoidsCancerEpithelial cellsCell marker genesStem cell marker genesSignificant increaseGenetic depletionWnt ligandsStem cellsProgressionLgr5StemnessCellsExpressionSequential mutationsMutations
2005
Mouse Taste Buds Use Serotonin as a Neurotransmitter
Huang Y, Maruyama Y, Lu K, Pereira E, Plonsky I, Baur J, Wu D, Roper S. Mouse Taste Buds Use Serotonin as a Neurotransmitter. Journal Of Neuroscience 2005, 25: 843-847. PMID: 15673664, PMCID: PMC6725637, DOI: 10.1523/jneurosci.4446-04.2005.Peer-Reviewed Original ResearchConceptsTaste budsPrimary sensory afferent fibersSensory afferent fibersBitter stimulationTransmitter release mechanismMouse taste budsAfferent fibersTransmitter candidatesGustatory receptor cellsIntracellular Ca2SerotoninSour tastantsReceptor cellsChinese hamster ovary cellsHamster ovary cellsNeurotransmittersSynapsesStimulationOvary cellsReleaseCa2CellsAcetylcholineCNSReceptors
1999
Dishevelled proteins lead to two signaling pathways. Regulation of LEF-1 and c-Jun N-terminal kinase in mammalian cells.
Li L, Yuan H, Xie W, Mao J, Caruso A, Sussman D, Wu D. Dishevelled proteins lead to two signaling pathways. Regulation of LEF-1 and c-Jun N-terminal kinase in mammalian cells. Journal Of Biological Chemistry 1999, 274: 33178. DOI: 10.1016/s0021-9258(17)46631-2.Peer-Reviewed Original Research
1998
Tec/Bmx non‐receptor tyrosine kinases are involved in regulation of Rho and serum response factor by Gα12/13
Mao J, Xie W, Yuan H, Simon M, Mano H, Wu D. Tec/Bmx non‐receptor tyrosine kinases are involved in regulation of Rho and serum response factor by Gα12/13. The EMBO Journal 1998, 17: 5638-5646. PMID: 9755164, PMCID: PMC1170892, DOI: 10.1093/emboj/17.19.5638.Peer-Reviewed Original ResearchConceptsNon-receptor tyrosine kinase familySerum response factorExpression of Galpha12SRF activationRho-independent pathwayThrombin effectTyrosine kinase familyActivation of SRFNon-receptor tyrosine kinaseG12/13 proteinsRho-dependent pathwayInvolvement of RhoTec kinasesActive alpha subunitG12 familyActivationTransient transfection systemTyrosine kinaseAlpha subunitCellsNIH 3T3 cellsKinase-deficient mutantKinaseRegulation of Rho
1996
Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma.
Kuang Y, Wu Y, Smrcka A, Jiang H, Wu D. Identification of a phospholipase C beta2 region that interacts with Gbeta-gamma. Proceedings Of The National Academy Of Sciences Of The United States Of America 1996, 93: 2964-2968. PMID: 8610151, PMCID: PMC39743, DOI: 10.1073/pnas.93.7.2964.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, CDBlotting, WesternCell LineChlorocebus aethiopsDNA, ComplementaryGlutamic AcidGTP-Binding ProteinsHumansIsoenzymesKidneyMacromolecular SubstancesMammalsMutagenesis, Site-DirectedPoint MutationPolymerase Chain ReactionReceptor, Anaphylatoxin C5aReceptors, ComplementRecombinant Fusion ProteinsRecombinant ProteinsTransfectionType C PhospholipasesConceptsCOS-7 cellsGlutathione S-transferaseGbeta gammaPLC-beta2Fusion proteinBeta gamma subunitsLigand-induced activationAlpha1B-adrenergic receptorMammalian expression plasmidX boxG proteinsY boxExpression plasmidAmino acidsS-transferaseProteinSequenceResiduesCellsActivationBeta2GalphaGbetaC5a receptorCDNA
1993
The transforming activity of activated Gα12
Jiang H, Wu D, Simon M. The transforming activity of activated Gα12. FEBS Letters 1993, 330: 319-322. PMID: 8397105, DOI: 10.1016/0014-5793(93)80896-3.Peer-Reviewed Original Research