2022
The Role of PARP‐1 and NF‐κB in Bile‐Induced DNA Damage and Oncogenic Profile in Hypopharyngeal Cells
Doukas PG, Vageli DP, Judson BL. The Role of PARP‐1 and NF‐κB in Bile‐Induced DNA Damage and Oncogenic Profile in Hypopharyngeal Cells. The Laryngoscope 2022, 133: 1146-1155. PMID: 35791892, DOI: 10.1002/lary.30284.Peer-Reviewed Original ResearchConceptsHypopharyngeal cellsAcidic bileNF-κBNF-κB activationGamma-H2A histone family member XHypopharyngeal squamous cell carcinomaOncogenic profileNuclear factor kappa BPARP-1Squamous cell carcinomaB-cell lymphoma 2 expressionNF-κB transcriptional activityFactor kappa BDNA damageEnzyme-linked immunosorbent assayH2A histone family member XCell survivalDNA/RNA damageHypopharyngeal carcinogenesisReflux contentsAnti-apoptotic phenotypeCell carcinomaWidespread DNA damageBAY 11PARP-1 overexpression
2021
Targeting STAT3 prevents bile reflux‐induced oncogenic molecular events linked to hypopharyngeal carcinogenesis
Vageli DP, Doukas PG, Siametis A, Judson BL. Targeting STAT3 prevents bile reflux‐induced oncogenic molecular events linked to hypopharyngeal carcinogenesis. Journal Of Cellular And Molecular Medicine 2021, 26: 75-87. PMID: 34850540, PMCID: PMC8742186, DOI: 10.1111/jcmm.17011.Peer-Reviewed Original ResearchConceptsBcl-2 overexpressionTranscriptional activationTranscription factorsTranscriptional activitySignal transducerSTAT3 knockdownMolecular eventsCell survivalQPCR analysisLuciferase assaySTAT3 activationTargeting STAT3Oncogenic eventsAberrant activationHypopharyngeal carcinogenesisSTAT3 phosphorylationSTAT3Oncogenic effectsSTAT3 pathwayPharmacologic inhibitionPharmacological inhibitionSTA-21Hypopharyngeal cellsOncogenic profileWestern blot
2020
The in vivo preventive and therapeutic properties of curcumin in bile reflux‐related oncogenesis of the hypopharynx
Doukas SG, Doukas PG, Sasaki CT, Vageli D. The in vivo preventive and therapeutic properties of curcumin in bile reflux‐related oncogenesis of the hypopharynx. Journal Of Cellular And Molecular Medicine 2020, 24: 10311-10321. PMID: 32691972, PMCID: PMC7521262, DOI: 10.1111/jcmm.15640.Peer-Reviewed Original ResearchConceptsNF-κBRecent pre-clinical studiesHuman hypopharyngeal cellsIndependent risk factorPre-clinical studiesNF-κB activationTherapeutic propertiesHypopharyngeal cellsRisk factorsMolecular eventsHypopharyngeal mucosaBile exposureImmunohistochemical analysisFuture translational developmentBile acidsOncogenic profileHypopharynxCarcinogenic effectsOncogenic functionOverexpression of RelAEpithelial cellsBileTranslational developmentExpression of genesCurcumin