2023
Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark
Gonçalves L, Angelé-Martínez C, Premi S, Palmatier M, Prado F, Di Mascio P, Bastos E, Brash D. Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark. ACS Chemical Biology 2023, 18: 484-493. PMID: 36775999, PMCID: PMC10276651, DOI: 10.1021/acschembio.2c00787.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNADNA DamageHormonesMammalsMelaninsNeurotransmitter AgentsPeroxynitrous AcidPyrimidine DimersUltraviolet RaysConceptsSinglet molecular oxygenOxidation of serotoninMolecular oxygenElectron excitationTriplet stateAdjacent pyrimidine basesAbsence of lightEnergy transferDark processPyrimidine basesSkin pigment melaninBiochemical reactionsMoleculesEnergy levelsCatecholamine neurotransmittersBiomoleculesCycloadditionUltravioletMammalian metabolismCyclobutane pyrimidine dimersOxidationAminesPigment melaninRadicalsPeroxynitriteChemiexcitation: Mammalian Photochemistry in the Dark†
Brash D, Goncalves L. Chemiexcitation: Mammalian Photochemistry in the Dark†. Photochemistry And Photobiology 2023, 99: 251-276. PMID: 36681894, PMCID: PMC10065968, DOI: 10.1111/php.13781.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsExcited statesMammalian cellsCyclobutane pyrimidine dimersEvolutionary selectionBond rearrangementUnoccupied orbitalsGround stateReaction productsPyrimidine dimersChemiexcitationRecent findingsBiologyPathogenic eventsRadicalsUltraviolet lightMoleculesDrug-induced deafnessPotential pathogenesisCellsMelaninAchilles heelMammalsBiomoleculesPhotochemistryDNA
2022
Cyclobutane Pyrimidine Dimer Hyperhotspots as Sensitive Indicators of Keratinocyte UV Exposure†
Garcia‐Ruiz A, Kornacker K, Brash DE. Cyclobutane Pyrimidine Dimer Hyperhotspots as Sensitive Indicators of Keratinocyte UV Exposure†. Photochemistry And Photobiology 2022, 98: 987-997. PMID: 35944237, PMCID: PMC9802031, DOI: 10.1111/php.13683.Peer-Reviewed Original ResearchMeSH KeywordsDNA DamageHumansInfant, NewbornKeratinocytesPyrimidine DimersReproducibility of ResultsTranscription FactorsUltraviolet RaysConceptsCyclobutane pyrimidine dimersGenomic averageSequence motifsHigh-throughput DNA sequencing methodsETS family transcription factorsNucleotide resolution analysisRNA processing genesNeonatal human epidermal keratinocytesDNA sequencing methodsTranscription factorsCpG islandsSites hundredsCell physiologyProcessing genesPromoter regionCell deathDNA damageSequencing methodsBiological importanceHuman epidermal keratinocytesPyrimidine dimersGenesMotifEpidermal keratinocytesMelanocytes
2019
Acetyl zingerone: An efficacious multifunctional ingredient for continued protection against ongoing DNA damage in melanocytes after sun exposure ends
Chaudhuri RK, Meyer T, Premi S, Brash D. Acetyl zingerone: An efficacious multifunctional ingredient for continued protection against ongoing DNA damage in melanocytes after sun exposure ends. International Journal Of Cosmetic Science 2019, 42: 36-45. PMID: 31538664, PMCID: PMC7004018, DOI: 10.1111/ics.12582.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDNA DamageEnvironmental ExposureGuaiacolMelanocytesMiceMice, Inbred C57BLPyrimidine DimersSunlightConceptsSun exposureSolar-simulated ultraviolet radiationReactive oxygen speciesIntracellular levelsCyclobutane pyrimidine dimersΑ-tocopherolCPD formationTraditional sunscreensScavenge peroxynitriteUVR exposureOngoing DNA damageAntioxidant α-tocopherolUltraviolet radiationUVA radiationMelanocytesROS formationExposureQuench singlet oxygenUse of AZEfficacyOxygen speciesKeratinocytesDNA damageFree radicalsHoursGenomic sites hypersensitive to ultraviolet radiation
Premi S, Han L, Mehta S, Knight J, Zhao D, Palmatier MA, Kornacker K, Brash DE. Genomic sites hypersensitive to ultraviolet radiation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 24196-24205. PMID: 31723047, PMCID: PMC6883822, DOI: 10.1073/pnas.1907860116.Peer-Reviewed Original ResearchMeSH Keywords5' Untranslated RegionsCells, CulturedDNA DamageFibroblastsGene Expression RegulationGenome, HumanHigh-Throughput Nucleotide SequencingHumansMelanocytesMelanomaMutationPromoter Regions, GeneticProtein BiosynthesisPyrimidine DimersPyrimidine NucleotidesSkin NeoplasmsTOR Serine-Threonine KinasesUltraviolet RaysConceptsCyclobutane pyrimidine dimersETS family transcription factorsIndividual gene promotersFamily transcription factorsRNA-binding proteinPrimary human melanocytesSingle-base resolutionEpigenetic marksGenomic averageTranslation regulationGenomic sitesMotif locationsTranscription factorsCell physiologyGene promoterCancer driversGenomeHuman melanocytesCell typesTumor evolutionCell pathwaysRare mutationsUV targetPyrimidine dimersApurinic sites
2016
Chemical excitation of electrons: A dark path to melanoma
Premi S, Brash DE. Chemical excitation of electrons: A dark path to melanoma. DNA Repair 2016, 44: 169-177. PMID: 27262612, PMCID: PMC4958542, DOI: 10.1016/j.dnarep.2016.05.023.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2015
Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure
Premi S, Wallisch S, Mano CM, Weiner AB, Bacchiocchi A, Wakamatsu K, Bechara EJ, Halaban R, Douki T, Brash DE. Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure. Science 2015, 347: 842-847. PMID: 25700512, PMCID: PMC4432913, DOI: 10.1126/science.1256022.Peer-Reviewed Original ResearchConceptsDark cyclobutane pyrimidine dimersExcited electronic statesUltraviolet photonsUV photonsElectronic statesTriplet stateSunlight-induced melanomaCytosine-containing cyclobutane pyrimidine dimersEnergy transferPhotonsPicosecondsElectronsUV exposureRadiationChemiexcitationEnergyStatePhotoproducts
2009
Influence of cytosine methylation on ultraviolet-induced cyclobutane pyrimidine dimer formation in genomic DNA
Rochette PJ, Lacoste S, Therrien JP, Bastien N, Brash DE, Drouin R. Influence of cytosine methylation on ultraviolet-induced cyclobutane pyrimidine dimer formation in genomic DNA. Mutation Research/Fundamental And Molecular Mechanisms Of Mutagenesis 2009, 665: 7-13. PMID: 19427505, DOI: 10.1016/j.mrfmmm.2009.02.008.Peer-Reviewed Original ResearchConceptsLigation-mediated PCRX chromosomeFMR1 geneGenomic DNAInactive X chromosomeDimer formationCyclobutane pyrimidine dimer formationTumor suppressor genePyrimidine dimer formationConstitutive methylationCytosine methylationMethylated cytosineUnmethylated cytosinesSuppressor geneP53 tumor suppressor geneGenesMethylationCPD formationChromosomesCytosineDNAMutationsSunlight-induced mutationsDipyrimidine sitesPGK1
1995
Intragenic Domains of Strand-specific Repair inEscherichia coli
Kunala S, Brash D. Intragenic Domains of Strand-specific Repair inEscherichia coli. Journal Of Molecular Biology 1995, 246: 264-272. PMID: 7869378, DOI: 10.1006/jmbi.1994.0082.Peer-Reviewed Original ResearchConceptsTranscription-repair coupling factorTranscription start siteStart siteEscherichia coli genesUV-induced cyclobutane pyrimidine dimersStrand-specific repairMfd mutationExcision repair mechanismChromatin domainsIntragenic domainsGenomic organizationMfd geneColi geneCyclobutane pyrimidine dimersLacZ transcriptionDNA repairInEscherichia coliDownstream domainIndividual nucleotidesRepair mechanismsGenesTranscriptionRepair systemDNA strandsInduced levels
1992
Excision repair at individual bases of the Escherichia coli lacI gene: relation to mutation hot spots and transcription coupling activity.
Kunala S, Brash DE. Excision repair at individual bases of the Escherichia coli lacI gene: relation to mutation hot spots and transcription coupling activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 11031-11035. PMID: 1438309, PMCID: PMC50477, DOI: 10.1073/pnas.89.22.11031.Peer-Reviewed Original ResearchConceptsTranscribed strandMutation hot spotsChromosomal DNAExcision repairIndividual nucleotidesEscherichia coli chromosomeExcision repair systemColi chromosomeMfd geneDistribution of mutationsGene fragmentsDNA fragmentsEnzymatic incisionCell extractsLacI geneUV-induced mutationsSite of labelingEscherichia coliRepair systemSpecific gene fragmentsUV photoproductsGenesBase variationsMutationsNucleotides
1990
Rapid repair kinetics of pyrimidine(6–4)pyrimidone photoproducts in human cells are due to excision rather than conformational change
Mitchell D, Brash D, Nairn R. Rapid repair kinetics of pyrimidine(6–4)pyrimidone photoproducts in human cells are due to excision rather than conformational change. Nucleic Acids Research 1990, 18: 963-971. PMID: 2315046, PMCID: PMC330351, DOI: 10.1093/nar/18.4.963.Peer-Reviewed Original Research
1989
Ultraviolet photoproducts at the ochre suppressor mutation site in the gln U gene of Escherichia coli: Relevance to “mutation frequency decline”
Garvey N, Witkin E, Brash D. Ultraviolet photoproducts at the ochre suppressor mutation site in the gln U gene of Escherichia coli: Relevance to “mutation frequency decline”. Molecular Genetics And Genomics 1989, 219: 359-364. PMID: 2695824, DOI: 10.1007/bf00259607.Peer-Reviewed Original Research
1987
Photoproduct frequency is not the major determinant of UV base substitution hot spots or cold spots in human cells.
Brash DE, Seetharam S, Kraemer KH, Seidman MM, Bredberg A. Photoproduct frequency is not the major determinant of UV base substitution hot spots or cold spots in human cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1987, 84: 3782-3786. PMID: 3473483, PMCID: PMC304960, DOI: 10.1073/pnas.84.11.3782.Peer-Reviewed Original Research
1986
Substrate range of the 40,000-dalton DNA-photoreactivating enzyme from Escherichia coli.
Sutherland B, Oliveira O, Ciarrocchi G, Brash D, Haseltine W, Lewis R, Hanawalt P. Substrate range of the 40,000-dalton DNA-photoreactivating enzyme from Escherichia coli. Biochemistry 1986, 25: 681-7. PMID: 3513832, DOI: 10.1021/bi00351a026.Peer-Reviewed Original Research
1982
New Approaches to DNA Damage and Repair: The Ultraviolet Light Example
Haseltine W, Gordon L, Lindan C, Lippke J, Brash D, Lo K, Royer-Pokora B. New Approaches to DNA Damage and Repair: The Ultraviolet Light Example. 1982, 20: 315-332. PMID: 7115270, DOI: 10.1007/978-1-4613-3476-7_21.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1981
Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.
Lippke JA, Gordon LK, Brash DE, Haseltine WA. Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences. Proceedings Of The National Academy Of Sciences Of The United States Of America 1981, 78: 3388-3392. PMID: 6943547, PMCID: PMC319573, DOI: 10.1073/pnas.78.6.3388.Peer-Reviewed Original Research