2023
Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark
Gonçalves L, Angelé-Martínez C, Premi S, Palmatier M, Prado F, Di Mascio P, Bastos E, Brash D. Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark. ACS Chemical Biology 2023, 18: 484-493. PMID: 36775999, PMCID: PMC10276651, DOI: 10.1021/acschembio.2c00787.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNADNA DamageHormonesMammalsMelaninsNeurotransmitter AgentsPeroxynitrous AcidPyrimidine DimersUltraviolet RaysConceptsSinglet molecular oxygenOxidation of serotoninMolecular oxygenElectron excitationTriplet stateAdjacent pyrimidine basesAbsence of lightEnergy transferDark processPyrimidine basesSkin pigment melaninBiochemical reactionsMoleculesEnergy levelsCatecholamine neurotransmittersBiomoleculesCycloadditionUltravioletMammalian metabolismCyclobutane pyrimidine dimersOxidationAminesPigment melaninRadicalsPeroxynitriteChemiexcitation: Mammalian Photochemistry in the Dark†
Brash D, Goncalves L. Chemiexcitation: Mammalian Photochemistry in the Dark†. Photochemistry And Photobiology 2023, 99: 251-276. PMID: 36681894, PMCID: PMC10065968, DOI: 10.1111/php.13781.Peer-Reviewed Original ResearchConceptsExcited statesMammalian cellsCyclobutane pyrimidine dimersEvolutionary selectionBond rearrangementUnoccupied orbitalsGround stateReaction productsPyrimidine dimersChemiexcitationRecent findingsBiologyPathogenic eventsRadicalsUltraviolet lightMoleculesDrug-induced deafnessPotential pathogenesisCellsMelaninAchilles heelMammalsBiomoleculesPhotochemistryDNA
2001
Escaping the stem cell compartment: Sustained UVB exposure allows p53-mutant keratinocytes to colonize adjacent epidermal proliferating units without incurring additional mutations
Zhang W, Remenyik E, Zelterman D, Brash D, Wikonkal N. Escaping the stem cell compartment: Sustained UVB exposure allows p53-mutant keratinocytes to colonize adjacent epidermal proliferating units without incurring additional mutations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 13948-13953. PMID: 11707578, PMCID: PMC61147, DOI: 10.1073/pnas.241353198.Peer-Reviewed Original ResearchThe DNA Damage Signal for Mdm2 Regulation, Trp53 Induction, and Sunburn Cell Formation In Vivo Originates from Actively Transcribed Genes
Brash D, Wikonkal N, Remenyik E, van der Horst G, Friedberg E, Cheo D, van Steeg H, Westerman A, van Kranen H. The DNA Damage Signal for Mdm2 Regulation, Trp53 Induction, and Sunburn Cell Formation In Vivo Originates from Actively Transcribed Genes. Journal Of Investigative Dermatology 2001, 117: 1234-1240. PMID: 11710938, DOI: 10.1046/j.0022-202x.2001.01554.x.Peer-Reviewed Original ResearchConceptsDNA photoproductsDNA damage signalsUnrepaired DNA lesionsCell formationSpecific genome regionsTumor suppressor proteinCsb-/- miceUltraviolet-induced apoptosisNucleotide excision repair genesApoptosis signal pathwayExcision repair genesActive genesMutant cellsGenome regionsDNA repairSuppressor proteinDamage signalsMDM2 regulationWild typeDNA lesionsPrevents cellsHomozygous inactivationGenesRepair genesDNA signalsTransgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53
Grossman D, Kim P, Blanc-Brude O, Brash D, Tognin S, Marchisio P, Altieri D. Transgenic expression of survivin in keratinocytes counteracts UVB-induced apoptosis and cooperates with loss of p53. Journal Of Clinical Investigation 2001, 108: 991-999. PMID: 11581300, PMCID: PMC200956, DOI: 10.1172/jci13345.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisChromosomal Proteins, Non-HistoneGene ExpressionHumansInhibitor of Apoptosis ProteinsKeratin-14KeratinocytesKeratinsMiceMice, KnockoutMice, TransgenicMicrotubule-Associated ProteinsNeoplasm ProteinsPhenotypePromoter Regions, GeneticSkinSurvivinTumor Suppressor Protein p53Ultraviolet Rays
2000
Regulation of TNFα production and release in human and mouse keratinocytes and mouse skin after UV‐B irradiation
Yarosh D, Both D, Kibitel J, Anderson C, Elmets C, Brash D, Brown D. Regulation of TNFα production and release in human and mouse keratinocytes and mouse skin after UV‐B irradiation. Photodermatology Photoimmunology & Photomedicine 2000, 16: 263-270. PMID: 11132130, DOI: 10.1034/j.1600-0781.2000.160606.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtaxia Telangiectasia Mutated ProteinsCell Cycle ProteinsCell LineCell MembraneEnzyme-Linked Immunosorbent AssayGene Expression RegulationGenes, fosGenes, p53HomozygoteHumansInterleukin-1KeratinocytesMaleMiceMice, Inbred C57BLMice, KnockoutProtein Serine-Threonine KinasesRadiation DosageReceptors, Tumor Necrosis FactorReverse Transcriptase Polymerase Chain ReactionSignal TransductionSirolimusSkinTranscription Factor AP-1Tumor Necrosis Factor-alphaUltraviolet RaysConceptsP53 knockout miceKnockout miceMembrane-bound TNFalphaHomozygous p53 knockout miceC-Fos signalingWild-type miceGene knockout miceRelease of TNFalphaTNFalpha gene expressionAP-1Cultured human keratinocytesImmunosuppressive responseCell culture supernatantsAP-1 transcription factorCultured epidermal cellsIL-1alphaCytokine TNFalphaTNFα productionType micePrimary cytokineTNFalpha inductionTNFalphaBasal levelsMouse skinMice
1999
Ultraviolet Radiation Induced Signature Mutations in Photocarcinogenesis
Wikonkal N, Brash D. Ultraviolet Radiation Induced Signature Mutations in Photocarcinogenesis. Journal Of Investigative Dermatology Symposium Proceedings 1999, 4: 6-10. PMID: 10537000, DOI: 10.1038/sj.jidsp.5640173.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAdultAgedCarcinoma, Squamous CellDNAHumansKeratinocytesMaleMiddle AgedNeoplasms, Radiation-InducedSkin NeoplasmsUltraviolet RaysConceptsSignature mutationsSkin cancerNon-melanoma skin cancerUV-signature mutationsClinical dataSubstantial burdenSkin carcinogenesisMurine epidermisNormal individualsNormal humansCancerCancer developmentTumor suppressor geneClonal expansionTumor promoterTP53Suppressor geneGenetic eventsMutationsCellsUV Induces p21WAF1/CIP1 Protein in Keratinocytes Without p53
Liu M, Wikonkal N, Brash D. UV Induces p21WAF1/CIP1 Protein in Keratinocytes Without p53. Journal Of Investigative Dermatology 1999, 113: 283-284. PMID: 10469320, DOI: 10.1046/j.1523-1747.1999.00657.x.Peer-Reviewed Original Research
1997
Sunlight and the onset of skin cancer
Brash D. Sunlight and the onset of skin cancer. Trends In Genetics 1997, 13: 410-414. PMID: 9351343, DOI: 10.1016/s0168-9525(97)01246-8.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1996
Relationship Between Sunlight Exposure and a Key Genetic Alteration in Basal Cell Carcinoma
Gailani M, Leffell D, Ziegler A, Gross E, Brash D, Bale A. Relationship Between Sunlight Exposure and a Key Genetic Alteration in Basal Cell Carcinoma. Journal Of The National Cancer Institute 1996, 88: 349-354. PMID: 8609643, DOI: 10.1093/jnci/88.6.349.Peer-Reviewed Original ResearchConceptsBasal cell carcinomaLoss of heterozygosityCell carcinomaP53 geneSunlight exposureExact testGenetic alterationsPathogenesis of BCCSun-exposed areasFrequency of LOHMohs micrographic surgical techniqueEnvironmental agentsLocation of tumorFisher's exact testSkin cancer patientsKey genetic alterationsUVB radiationChi-squared analysisFrequent genetic alterationsLimited associationSpecific environmental agentsBCC incidenceTumor characteristicsCancer patientsCommon cancerWrinkles waiting for GOD0T
Brash D, Gilchrest B. Wrinkles waiting for GOD0T. Nature 1996, 379: 301-302. PMID: 8552182, DOI: 10.1038/379301a0.Commentaries, Editorials and Letters
1995
Intragenic Domains of Strand-specific Repair inEscherichia coli
Kunala S, Brash D. Intragenic Domains of Strand-specific Repair inEscherichia coli. Journal Of Molecular Biology 1995, 246: 264-272. PMID: 7869378, DOI: 10.1006/jmbi.1994.0082.Peer-Reviewed Original ResearchConceptsTranscription-repair coupling factorTranscription start siteStart siteEscherichia coli genesUV-induced cyclobutane pyrimidine dimersStrand-specific repairMfd mutationExcision repair mechanismChromatin domainsIntragenic domainsGenomic organizationMfd geneColi geneCyclobutane pyrimidine dimersLacZ transcriptionDNA repairInEscherichia coliDownstream domainIndividual nucleotidesRepair mechanismsGenesTranscriptionRepair systemDNA strandsInduced levels
1994
Sunburn and p53 in the onset of skin cancer
Ziegler A, Jonason A, Leffellt D, Simon J, Sharma H, Kimmelman J, Remington L, Jacks T, Brash D. Sunburn and p53 in the onset of skin cancer. Nature 1994, 372: 773-776. PMID: 7997263, DOI: 10.1038/372773a0.Peer-Reviewed Original ResearchConceptsActinic keratosisP53 mutationsSquamous cell carcinomaP53 tumor suppressor geneP53-mutated cellsCell carcinomaApoptotic keratinocytesSkin cancerTumor initiatorTumor suppressor geneMouse skinClonal expansionPrecancerous cellsTumor promoterCarcinomaSkinTissue responseP53SunburnCell differentiationDNA damageAberrant cell differentiationCellsResponseKeratosis
1990
Rapid repair kinetics of pyrimidine(6–4)pyrimidone photoproducts in human cells are due to excision rather than conformational change
Mitchell D, Brash D, Nairn R. Rapid repair kinetics of pyrimidine(6–4)pyrimidone photoproducts in human cells are due to excision rather than conformational change. Nucleic Acids Research 1990, 18: 963-971. PMID: 2315046, PMCID: PMC330351, DOI: 10.1093/nar/18.4.963.Peer-Reviewed Original Research
1989
Ultraviolet photoproducts at the ochre suppressor mutation site in the gln U gene of Escherichia coli: Relevance to “mutation frequency decline”
Garvey N, Witkin E, Brash D. Ultraviolet photoproducts at the ochre suppressor mutation site in the gln U gene of Escherichia coli: Relevance to “mutation frequency decline”. Molecular Genetics And Genomics 1989, 219: 359-364. PMID: 2695824, DOI: 10.1007/bf00259607.Peer-Reviewed Original Research
1988
UV MUTAGENIC PHOTOPRODUCTS IN Escherichia coli AND HUMAN CELLS: A MOLECULAR GENETICS PERSPECTIVE ON HUMAN SKIN CANCER*
Brash D. UV MUTAGENIC PHOTOPRODUCTS IN Escherichia coli AND HUMAN CELLS: A MOLECULAR GENETICS PERSPECTIVE ON HUMAN SKIN CANCER*. Photochemistry And Photobiology 1988, 48: 59-66. PMID: 3064116, DOI: 10.1111/j.1751-1097.1988.tb02786.x.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
1982
UV-induced mutation hotspots occur at DNA damage hotspots
Brash D, Haseltine W. UV-induced mutation hotspots occur at DNA damage hotspots. Nature 1982, 298: 189-192. PMID: 7045692, DOI: 10.1038/298189a0.Peer-Reviewed Original Research